摘要:

Thirty-two amenorrheic patients were treated with a tablet oral placebo preparation for a period varying from 30 to 180 days. Another 24 amenorrheic patients were also treated with a placebo administered i.m. for a period varying from 30 to 120 days. As a consequence of the treatment, 27 patients (48%) had menstrual bleedings. The progestogen withdrawal test responsive patients were more responsive to placebo (73 vs. 14% in the progestogen withdrawal test nonresponsive, p < 0.001). The time lag between starting the medication and the first bleeding varied between 4 and 120 days with a mean value of 3 3.9 (SD 26.3). Oral placebo was more effective than the intramuscular form (56 vs. 38%, p < 0.05).

ISSN:0378-7346    

DOI:10.1159/000292555

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

The aim of the present study was to investigate whether women with endometriosis displayed a decreased lymphokine-activated killer (LAK) activity. In 15 women with and 7 women without endometriosis the cytotoxicity against four different tumor cell lines – K562, the endometrium carcinomas AN3CA and RL95, the natural-killer (NK)-resistant Daudi cell line – was investigated, using either freshly isolated peripheral blood mononuclear cells (PBMC) or recombinant interleukin (IL)-2-stimulated PBMC. In 5 additional women collagenase-DNase-digested endometrium was used, to investigate whether recombinant IL-2-activated lymphocytes displayed an increased cytotoxicity against fresh and cultured endometrial cells. The cytotoxicity of unstimulated PBMC toward K562, AN3CA and RL95 target cells was decreased in women with endometriosis compared to women without endometriosis (p < 0.05, for all). After recombinant IL-2 stimulation the cytotoxicity toward the four different target cells increased significantly, both in women with and without endometriosis. There was no difference in LAK-mediated cytotoxicity against the four tumoral cells between women with and without endometriosis. Significant LAK activity was demonstrated against both fresh and cultured (72 h) endometrial cells. The cytotoxicity of autologous lymphocytes against cultured endometrial cells was 31.0+ 17 versus 67.4 ± 5.8%, using lymphocytes cultured in medium without and with recombinant IL-2, respectively (paired t test, p < 0.02). These results demonstrated that the decreased NK activity in peripheral blood of women with endometriosis was corrected after in vitro recombinant IL-2 stimulation of lymphocytes. Furthermore, this study showed that cytotoxicity toward fresh and cultured endometrial cells increased after in vitro incubation of PBMC with recombinant IL-2.

ISSN:0378-7346    

DOI:10.1159/000292556

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Thirty-one patients complaining of unexplained infertility for at least 36 months and diagnosed elsewhere were reassessed laparoscopically. After staining their pelvic peritoneum with concentrated methylene blue, they presented with extensive areas of dark blue discoloration, and were diagnosed as suffering from ‘diseased pelvic peritoneum’. The levels of peritoneal CA 125 were assessed. At the end of the diagnostic procedure, bipolar electrocoagulation or defocalized laser beam therapy was performed to destroy the affected peritoneal areas, and to allow peritoneal regeneration. Twenty-five pregnancies were obtained following this treatment. A detailed description is given of the diagnosis and treatment procedures.

ISSN:0378-7346    

DOI:10.1159/000292557

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Our objective was to apply a new surgical technique to ovarian cystectomy combining the advantages of laparoscopy with the benefits of microsurgical principles, in an attempt to simplify the surgical laparoscopic procedure and reduce postoperative adhesion formation. Between May 1991 and March 1992, extracorporal ovarian cystectomy was performed in 27 patients who presented with persistent ovarian cysts at the department of gynecology of a large teaching hospital that receives primary referrals from public health care patients. Patients were referred with the diagnosis of a persistent ovarian cyst. Diagnosis was confirmed by pelvic bimanual examination, and transvaginal ultrasound. Cases where malignancy was suspected due to the presence of ascites, semi-solid or solid masses, demonstration of papillations within the ovarian cyst on ultrasound, or elevated CA-125 levels (unless endometriosis was suspected) were excluded from the study group. At laparoscopy, after inspection of the abdominal cavity the cyst was aspirated. The ovary was then extracted through a 2- to 3-cm lower-abdominal incision, cystectomy was meticulously performed according to microsurgical principles, and the repaired ovary was then returned into the abdominal cavity. Extracorporal cystectomy was successfully performed in 26 of 27 cases. The mean duration of the operative procedure was 55 min. The postoperative course was uneventful. Mean postoperative hospi-talization time was 22.4 h. Over a follow-up period of up to 15 months, ovarian folliculogenesis was confirmed ultrasonographically. In the first 2 patients to undergo second-look laparoscopy, no adhesions were seen. Extra-abdominal ovarian cystectomy is a safe procedure, easily mastered, and a simpler surgical procedure than that of operative laparoscopic ovarian cystectomy, which may be advantageous for the prevention of postoperative adhesions.

ISSN:0378-7346    

DOI:10.1159/000292558

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

CA125 and CA130 in various tissues were characterized using the monoclonal antibodies OC125, 130-22, and 145-9. CA125 and CA130 were eluted in the same fraction in 130-22 affinity chromatography. A sandwich immunoradiometric assay using these antibodies in combination was established. When the solid phase of the assay was changed from OC125 to 145-9, the amount of 125I-OC125 that reacted with CA125 in the amnion increased more than that in other samples. On SDS-PAGE, CA125 and CA130 epitopes in the amnion migrated to different positions. These results indicate that CA125 and CA130 are located on different subunits of the same glycoprotein, and that their synthesis is regulated differently in various tissues.

ISSN:0378-7346    

DOI:10.1159/000292559

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

The objective was to evaluate natural cytotoxicity of peripheral blood during interferon treatment in cervical intraepithelial neoplasia grade 2 (CIN2), as index of interferon activity. Twenty-one patients with CIN2, histologically proven, were treated with interferon α 2b (Intron A, Sheering-Plough Corp.), in a dose of 3,000,000 units three times per week for 8 weeks self-administered by intramuscular injection. The rate of remission to the interferon treatment was 38.1%. Eight healthy patients had a significant increase in natural killer activity during and after the treatment (p < 0.001), whereas the 13 nonresponder patients remained with low values of natural killer activity. By analyzing the basal natural killer activity before the treatment, the patients with clinic pathologic remission had a significantly higher mean value than nonresponder patients (p = 0.007). Notwithstanding the specimen exiguity, the individual basal natural killer activity seems to be a predictive parameter of interferon treatment response in patients with CIN2.

ISSN:0378-7346    

DOI:10.1159/000292560

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Two human choriocarcinoma cell lines, BeWo and Jar, were used as a model system to study 17β-hydroxysteroid dehydrogenase (17β-HSD) activity. Northern blot, Western blot and 3H-water assay were performed to investigate the mRNA species, protein and enzyme activity of 17β-HSD. Two sizes of 17β-HSD mRNA, 1.3 and 2.2 kb, were detected in BeWo and Jar cells. In BeWo cells the predominant mRNA species is 1.3 kb. Western blot analysis demonstrated high level of 17β-HSD immunoreactivity and the 3H-water assay demonstrated significant enzyme activity in BeWo cells. In contrast, in Jar cells, the predominant mRNA species is 2.2 kb. Jar cells contain very low 17β-HSD enzyme activity and Western blot failed to show detectable 17β-HSD immunoreactivity. It is possible that the 2.2-kb 17β-HSD mRNA cannot be efficiently translated into protein because of the presence of multiple AUGs and termination signals (UGAs, UAAs, and UAGs) at the 5’-untranslated region of the 2.2-kb mRNA.

ISSN:0378-7346    

DOI:10.1159/000292561

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

The improvement in rehabilitation of young women with spinal cord lesions, combined with the fact that libido, fertility and child bearing remain intact, enables more such patients to conceive. The obstetric team is therefore bound to meet with such patients. Among the various complications of this condition during pregnancy is an acute life-threatening syndrome called autonomic hyperrefiexia (AH), caused by an uncontrolled massive sympathetic discharge, which occurs in response to stimuli that reach the spinal cord below the lesion. Most authors agree that the best method of controlling AH during labor is by an epidural block which impends stimuli from reaching the spinal cord and thus provoking AH. Most authors could not completely abolish AH episodes using different medication epidurally. Herein we present a successful delivery using a combination of morphine bupivacaine epidurally.

ISSN:0378-7346    

DOI:10.1159/000292562

出版商:S. Karger AG    

年代:1994

数据来源: Karger

ISSN:0378-7346    

DOI:10.1159/000290789

出版商:S. Karger AG    

年代:1913

数据来源: Karger

ISSN:0378-7346    

DOI:10.1159/000290790

出版商:S. Karger AG    

年代:1913

数据来源: Karger