摘要:

To assess the circulation of human herpesvirus (HHV)-8 infection over the years, two seroprevalence surveys were conducted, which tested sera from HIV-infected individuals recruited 10 years apart (206 individuals from 1986 to 1988 and 177 individuals from 1997 to 1998). For all patients, antibodies to hepatitis C virus (HCV), hepatitis B virus (HBV), and HHV-8 lytic and latent antigens were evaluated.HHV-8 seroprevalence was higher among individuals recruited in the 1990s (31.6% for anti-lytic, 8.5% for anti-latent antibodies) compared with similar findings in those seen in the late 1980s (14.6% and 3.4% for anti-lytic and anti-latent antibodies, respectively), with a twofold increase of the risk of HHV-8 infection. However, the increase was observed only among injecting drug users, whereas seroprevalence tended to slightly increase among those infected by sexual contact. At univariate analysis, time of recruitment and being homosexual men were factors associated with HHV-8 infection, an association that remained after adjusting for age. HBV infection was significantly associated with HHV-8 infection (odds ratio [OR], 2.2; 95% confidence interval [CI], 1.3–3.6), whereas those infected with HCV had a lower probability of having HHV-8 antibodies (OR, 0.3; 95% CI, 0.20–0.6). After controlling for age and gender, time of recruitment remained independently associated with HHV-8 infection among injecting drug users.In conclusion, HHV-8 seroprevalence appears to be increased during 10 years among HIV-infected injection drug users but not among homosexual men, who remain those at the highest risk of infection.

ISSN:1525-4135    

出版商:OVID    

年代:2002

数据来源: OVID

摘要:

We report in this study the use of polymerase chain reaction (PCR) for the amplification of the genomic DNA, isolated from thymic tissue, using the primers flanking HTLV-I/IItax-rexgenes and the sequence method to analyze the HTLV-Ipolsequence of 27 Italian patients with myasthenia gravis. These molecular methods showed that 92.5% of patients tested positive fortaxgene and 55% forpolgenes; 55.5% samples were positive for both thetaxgene of HTLV-I/II, and thepolgene of HTLV-I. Histologic investigation of the thymus showed that 15 samples had thymic hyperplasia, 93% tested positive for thetaxgene, and 40% tested positive for both thetaxandpolgenes of HTLV-I. In contrast, 91.6% of thymoma-positive samples were positive for tax gene I/II and 75% positive for both genes,taxandpoltype I. The sequence analysis of PCR product fortaxandpolgenes confirmed that these amplified products were HTLV-I, with minimal variations. Our date suggested that either HTLV-I or part of the virus genome is involved in the etiopathogenesis of myasthenia gravis.

ISSN:1525-4135    

出版商:OVID    

年代:2002

数据来源: OVID

摘要:

Homozygosity for the 32 base-pair deletion (&Dgr;32/&Dgr;32) in the CCR5 coreceptor gene is associated with incomplete HIV-1 resistance. Six HIV-1–infected &Dgr;32/&Dgr;32 patients have been reported. We report 2 additional &Dgr;32/&Dgr;32-infected individuals, among 106 seroconverters in a vaccine preparedness study. Like the previous 6, these individuals experienced rapid CD4 decline. However, taken together, the 8 patients have neither uniformly high virus load nor rapid progression to AIDS. We obtained five virus isolates from 1 patient at 5, 6, 7, 10, and 12 months after the estimated time of infection. The earliest isolate exhibits the syncytium-inducing (SI) phenotype and exclusive use of the CXCR4 coreceptor, suggesting acquisition of HIV-1 through this coreceptor. Of the remaining 104 seroconverters, 8 were CCR5-&Dgr;32/+ and 96 were CCR5-+/+. Three CCR5-+/+seroconverters who showed the uncommon pattern of early SI virus and rapid CD4 decline had uniformly high viral load and more heterogeneous coreceptor usage. These results further support the conclusion that &Dgr;32-mediated resistance is incomplete and is associated with acquisition of exclusively-X4 variants of HIV-1. The pathogenic potential of these viruses may be different from late-stage X4 virus or early X4 virus acquired by individuals with other CCR5 genotypes.

ISSN:1525-4135    

出版商:OVID    

年代:2002

数据来源: OVID

ISSN:1525-4135    

出版商:OVID    

年代:2002

数据来源: OVID

ISSN:1525-4135    

出版商:OVID    

年代:2002

数据来源: OVID

ISSN:1525-4135    

出版商:OVID    

年代:2002

数据来源: OVID

ISSN:1525-4135    

出版商:OVID    

年代:2002

数据来源: OVID

ISSN:1525-4135    

出版商:OVID    

年代:2002

数据来源: OVID