ISSN:0893-5785    

DOI:10.1111/j.1600-0749.1991.tb00307.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0893-5785    

DOI:10.1111/j.1600-0749.1991.tb00308.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Modern cancerology is based on the oncogene concept. This is rather new. The idea of the oncogene, however, is old, and can be traced back to two sources, namely to “cancer families,” reported in 1866 by P. Broka, and to “virus induced” neoplasia, detected by P. Rous in 1911. A gene which is—to my knowledge—the first reported oncogene by definition was detected in the little ornamental Mexican fishXiphophorusby Myron Gordon, Curt Kosswig, and Georg Häussler in 1928 when they observed the terrible hereditary melanomas that we are now coming to understand and to compare with other kinds of neoplasms inXiphophorusand in mammals, including humans. Although theXiphophorusmodel was always modest in its claims, it has—sometimes too early in its history—contributed many facts to the present conc

ISSN:0893-5785    

DOI:10.1111/j.1600-0749.1991.tb00309.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Eighteen hair samples from Karakul newborn lambs with various colors were estimated for eumelanin and pheomelanin contents (Ceand Cp, respectively) by electron spin resonance (ESR) spectrometry and by high‐performance liquid chromatography (HPLC). Correlation coefficients between the values estimated by the ESR and HPLC methods were 0.96, 0.93, and 0.99 for Ce, Cp, and Ce/Cp, respectively. The high correlation coefficients show that both methods fit well for estimation of relative values of these parameters. The absolute values of Ceand Ce/Cpcoincide rather well when Ceis high, but considerable discrepancies appear when Ceis low. The reasons for these discrepancies are discussed. The HPLC method appears to be more sensitive for detection of low concentrations of pheomelanin, while the ESR method fits well for mass selection purpose

ISSN:0893-5785    

DOI:10.1111/j.1600-0749.1991.tb00310.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Nine metastatic melanoma cell lines and two melanocyte cell lines were analyzed for point mutations in highly conserved regions of thep53gene. No mutations were detected in the two melanocytic cell lines and in eight melanoma cell lines. However, a C→T transition at codon 248, resulting in a substitution of tryptophan for arginine, was found in one melanoma cell line. On immunohistochemical staining, only this cell line showed reactivity for mouse monoclonal antibody 1801, which is immunoreactive with humanp53protein. The original paraffin‐embedded specimen from which this mutant cell line was established was obtained, and sequence analysis detected the identical mutation in thep53gene as that seen in the derived cell line. This is the first report indicating point mutations in thep53gene in malignant melanocytic tiss

ISSN:0893-5785    

DOI:10.1111/j.1600-0749.1991.tb00311.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

A full‐length cDNA clone encoding a 115‐kDa melanosomal matrix protein (MMP115) was isolated from a cDNA library constructed from poly(A)+RNA of the chicken pigmented epithelial cells. Sequence analysis showed that the cDNA encoded a polypeptide of 762 amino acids, including a hydrophobic signal peptide. There are no membrane‐spanning regions, but there are five N‐linked glycosylation signals. A cysteine‐ and histidine‐rich domain is present near the C‐terminus. A sequence of 24 amino acids is repeated three times in the polypeptide. A database search for homologies yielded no sequence similarities in other proteins. A plasmid containing the full‐length cDNA was transferred into mouse cell lines by transfection. The transfected cells produced a protein that had the same size, 115 kDa, as the mature MMP115. When B16 mouse melanoma cells were transfected, the chicken MMP115 was expressed in the melanosomes. The presence of a specific sorting signal was suggested for localization of melanosomal proteins. Southern blot analysis has revealed that the homologues of the chicken MMP115 gene are found in many ver

ISSN:0893-5785    

DOI:10.1111/j.1600-0749.1991.tb00312.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Normal human skin is shown to contain melanocyte growth factor (MeGF). We found MeGF activity in extracts of both the epidermal portion of skin and the dermal portion. This activity was completely adsorbed onto heparin beads and eluted by 2.5 M NaCl. In addition, the activity of both extracts was completely blocked by antibodies directed against basic fibroblast growth factor (bFGF). It is suggested that melanocytes in epidermis are supported by bFGF‐like MeGF in normal human ski

ISSN:0893-5785    

DOI:10.1111/j.1600-0749.1991.tb00313.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0893-5785    

DOI:10.1111/j.1600-0749.1991.tb00314.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY