摘要:

&NA;Progress has been made in the past year in the development of new or improved diagnostic techniques based on the use of monoclonal antibodies, DNA probes, and the polymerase chain reaction (PCR). In this review, emphasis is placed on new rapid tests that can be incorporated into the work flow of the clinical laboratory. In addition, promising new PCR‐based technologies are described that are aimed at the rapid detection of specific genetic sequences harbored by a variety of infectious agents. In all, 19 new PCR tests are described that target toxin genes ofClostridium difficile,enterotoxigenicEscherichia coli,enterohemorrhagicE. coli,virulence genes ofShigella,enteroinvasiveE. coli, Yersinia, Entamoeba histolytica,and the urease gene ofHelicobacter pylori.PCR tests for enteric adenoviruses and rotaviruses of groups A, B, and C are also described. All procedures are critically reviewed for levels of sensitivity, specificity, and impact on the diagnosis of enteric infectious diseases.Current Opinion in Gastroenterology 1992,8:134‐144

ISSN:0267-1379    

出版商:OVID    

年代:1992

数据来源: OVID

ISSN:0267-1379    

出版商:OVID    

年代:1992

数据来源: OVID

ISSN:0267-1379    

出版商:OVID    

年代:1992

数据来源: OVID