摘要:

AbstractThe examination of four species of luminous bacteriaPhotobacterium leiognathi, Photobacterium phosphoreum, Vibrio fischeriandVibrio harveyihas enabled us to reveal some nutrient medium components effecting growth, luminescence intensity and luciferase synthesis. These agents are nucleic components (nucleotides, nucleosides and amine bases), amino acids and vitamins, which are part of hydrolysates from the biomass of various lithotrophic microorganisms, hydrogen‐oxidizing, ironoxidizing and carboxydobacteria. The effect of promoting agents essentially alters the physiological state and ultrastructure of the cells of luminous bacteria and increases luciferase biosynthesis two‐ to three‐fold compared to a co

ISSN:0884-3996    

DOI:10.1002/bio.1170080602

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe luminescence of obelin is initiated by NaOCl in a reaction mixture containing no calcium. The addition of Mn2+enhances the light emission>300‐fold. Sodium azide and histidine, as singlet oxygen quenchers, inhibit NaOCl‐activated obelin luminescence in the presence or absence of Mn2+. This suggests that the addition of NaOCI to the mixture causes singlet oxygen formation (stimulated by Mn2+ions), and singlet oxygen initiates the light‐emitting rea

ISSN:0884-3996    

DOI:10.1002/bio.1170080603

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractTwo different mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite were found. Most substances investigated in these experiments acted by scavenging NaOCI. This mechanism was independent of the concentration of hydrogen peroxide and the incubation time between luminol and inhibitors. The most potent inhibitors were substances containing SH groups. Compounds with amino groups as a target for HOCI/OCI−to yield chloramines were much less effective inhibitors. Another mechanism of inhibition was found for catalase. It depended on the presence of hydrogen peroxide in the incubation medium and the incubation time between luminol and catalase. The enzyme inhibited the luminescence by removing H2O2at molar concentrations much smaller than those found for all other inhibitors. Our results confirm the present models of the mechanism of generation of luminescence in luminol oxidatio

ISSN:0884-3996    

DOI:10.1002/bio.1170080604

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractNetworks were prepared by curing diglycidyl ether of bisphenol A (DGEBA) with variable concentrations of norbornene anhydride (NA). Almost completely cured samples with anhydride/epoxide (A/E) inolar ratios of respectively 0.8, 0.9, 1.0 and 1.1, and one incompletely cured sample with A/E = 1.0, were studied by chemiluminescence in the temperature range 135–220°C, using isothermal stationary or non‐stationary (atmosphere change) exposures. The comparison of kinetic curves of intensity variation reveals: the importance of unreacted epoxide groups as sources of highly emissive radical species, the lowering effect of oxidation products, and the increasing effect of the decrease of macromolecular mobility due to crosslinking in the case of the incompletely cured sample. Most of the features of kinetic curves obtained in non‐stationary experiments are explained in terms of radical formation mechanisms during exposure in inert atmosphere. The results show clearly that chemiluminescence is due to reactions of peroxy radicals rather than hydroperoxide

ISSN:0884-3996    

DOI:10.1002/bio.1170080605

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe abstracts of the talks and posters at this recent two day meeting are reproduced below. An exhibit of equipment and reagents was also held concurrently with the meeting.

ISSN:0884-3996    

DOI:10.1002/bio.1170080606

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170080607

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170080608

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170080601

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY