摘要:

AbstractThis study has investigated the kinetics and mechanism of ultraweak luminescence in maize roots. Mannitol induced the second maximum and enhanced the main maximum of the relative intensity of luminescence from the roots. Hydroquinone and quinone enhanced the relative intensity of the luminescence. Catalase enhanced the maximum of the luminescence and changed the kinetics of the light emission. The effect of catalase on the kinetics was abolished by superoxide dismutase. Ascorbate in the presence of catalase reduced the luminescence maximum, but did not alter the kinetics. In the presence of catalase only, or in the combination with superoxide dismutase, or ascorbate, the luminescence intensity in the stationary phase was significantly lower compared to the control. The results support the participation of superoxide‐radical, singlet oxygen, electron transfer and the role of peroxidase in the reactions generating ultraweak luminescence in the roots. Ascorbate, catalase and superoxide dismutase have a protective role in the luminescent reaction

ISSN:0884-3996    

DOI:10.1002/bio.1170050403

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractChemiluminescence (CL) occurs during reactions of several of the components of the CH2(COOH)2KBrO3MnSO4H2SO4self‐sustained system. In contrast to the kinetics determined using potentiometry and spectrophotometry, the CL intensity kinetic curve during the oxidation of manganese (II) ions by acidic bromate has an extremum. Experimental dependences of the maximum CL intensity value on the initial reagent concentrations have been determined and compared with the results of the numerical simulation based on the commonly accepted Noyes‐Field‐Thompso

ISSN:0884-3996    

DOI:10.1002/bio.1170050404

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractLuminol‐enhanced chemiluminescence (CL) was used to examine the response of various leukocyte populations following stimulation with a crude extract ofPhaseolus vulgaris, namely phytohaemagglutinin (PHA‐C). Populations stimulated included a human peripheral mixed leukocyte preparation (MLP), and purified preparations of lymphocytes, monocytes and polymorphonuclear leukocytes (PMNL). Mouse peritoneal exudate cells and the lymphocytic cells lines Molt #4 and Daudi were also stimulated. Following stimulation, a characteristic three‐peaked chemiluminescent response was obtained from the MLP population. Little or no response was obtained from the purified lymphocytes. Monocytes produced a sharp peak corresponding to the second peak of the MLP response and PMNL produced a broad peak corresponding to the third peak of the MLP response. Mouse peritoneal exudate cells containing lymphocytes and monocytes/macrophages showed a two‐peaked stimulation which corresponded to the first two peaks of the MLP response. Molt #4 and Daudi showed no chemiluminescence if stimulated individually, but if added to a MLP substantial enhancement of the first and second peaks was observed. These results indicate some form of lymphocyte/monocyte interaction leading to enhanced CL following PHA‐C st

ISSN:0884-3996    

DOI:10.1002/bio.1170050405

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractA luminol‐dependent chemiluminescence assay for the assessment of the phagocytosis of erythrocytes sensitized with anti‐D IgG immunoglobulin by mononuclear leukocytes is described. The mononuclear leukocytes were obtained by apheresis enriched by centrifugation through a density gradient and stored in liquid nitrogen before use. The total reaction mixture, consisting of mononuclear leukocytes‐luminol‐erythrocytes (either anti‐D IgG sensitized or unsensitized controls) was 500 μl, light detection was by an LKB 1251 luminometer. Peak luminescence was seen between 35–45 minutes, the reaction being exhausted by 120 minutes. Determination of the reproducibility of the assay gave intra‐ and inter‐assay coefficients of variation of 5% and 13% respectively. We found the chemiluminescent response to be affected by the number of erythrocytes used in the assay and by the composition of the medium in which the cells were resuspended, particularly the pH at the initiation of the assay. We also compared the chemiluminescence assay to a microscopic phagocytic assay and found the results virtually identical. However, the former chemiluminescence assay was much easier to perform, marginally more sensitive, less laborious and eliminated any possibility of

ISSN:0884-3996    

DOI:10.1002/bio.1170050406

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170050407

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170050402

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY