摘要:

AbstractIn order to assess the importance of chlorine in a drug molecule as an influence on myeloperoxidase‐mediated inflammatory cell functions, the effect of the chlorinated bisphosphonate, clodronate, on human neutrophil chemiluminescence and myeloperoxidase (MPO) activity was compared to the non‐chlorinated structural analogue, etidronate. The results suggested that the presence of chlorine may be important to the enhancement of MPO activity. In addition both drugs manifested low toxicity and both of these observations may have relevance to host defe

ISSN:0884-3996    

DOI:10.1002/bio.1170060402

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractPhoton emission from germinating gram seeds at various stages of growth exhibits a definite pattern. The pattern of emission changes when a seed is disrupted by physical processes, e. g. mechanical crushing, cooling or heating. The disrupted seeds do not grow. The change in the biological order responsible for seed growth and the observed changes in the pattern of photo emission suggest a link between the macroscopic spatio‐temporal organization and metabolic processe

ISSN:0884-3996    

DOI:10.1002/bio.1170060403

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractEnhanced chemiluminescent assays for hydrolase enzymes have been developed using proehancer and pro‐anti‐enhancer substrates. Alkaline phosphatase is measured using disodiumpara‐iodophenyl phosphate (proenhancer) which is converted topara‐iodophenol and this in turn enhances the light emission from the horseradish peroxidase catalysed chemiluminescent oxidation of luminol by peroxide. An alternative strategy usespara‐nitrophenyl phosphate which is converted by alkaline phosphatase topara‐nitrophenol which inhibits the enhanced chemiluminescent reaction. The detection limit for the enzyme using the proenhancer and pro‐anti‐enhancer assays was 100 attomoles and 1 picornole, respectively. The proenhancer strategy was effective in assays for beta‐D‐galactosidase, beta‐D‐glucosidase and aryl sulfatase. A limited comparison of the proenhancer and a conventional colorimetric assay for an alkaline phosphatase label in an enzyme immunoassay for alpha‐fetoprot

ISSN:0884-3996    

DOI:10.1002/bio.1170060404

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThe intensity of 4‐I‐phenol‐enhanced chemiluminescence from the luminol‐H2O2‐horseradish peroxidase system is markedly attenuated in the presence of low concentrations of non‐enhancer phenols. Under the conditions studied, the effect is not associated with competition between 4‐I‐phenol and non‐enhancer phenol for the enzyme intermediates, Compounds I and II, but involves a competition between non‐enhancer phenol and luminol most probably for the

ISSN:0884-3996    

DOI:10.1002/bio.1170060405

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractTyrosine markedly attenuates the chemiluminescence output intensity from the 4‐iodophenol enhanced chemiluminescence assay system in a manner consistent with competition between the amino acid and luminol for the 4‐iodophenoxy radical. This effect provides the basis for a sensitive assay of tyrosine. Interference by the other amino acids has been assessed; major interference by cysteine can be removed by incubation with iodoacetic a

ISSN:0884-3996    

DOI:10.1002/bio.1170060406

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractAn asasy for alkaline phosphatase is described which is based on the hydrolysis of riboflavin phosphates (5′FMN or 4′FMN) to produce riboflavin. This is converted to 5′FMN using riboflavin kinase, and then asayed using the bacterial bioluminescent system fromVibrio harveyiorV. Fischeri.The most sensitive assay is obtained using 4′FMN, which can measure less than 20 amol after a 1‐hour i

ISSN:0884-3996    

DOI:10.1002/bio.1170060407

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractA simple, rapid and sensitive bioluminescent method has been used to measure total bile acids in hyperlipidaemic serum. We found that the levels of total bile acids in hypertriglyceridaemic and hypercholesterolemic sera determined by a spectrophotometric method were four‐fold higher than those measured by the bioluminescent method (6.73 ± 4.07 μmol/l (mean ± SD) by bioluminescent and 26.10 ± 13.42 μmol/l by the spectrophotometric method). There was no difference in total bile acid levels between these two methods for normal serum (4.72 ± 3.38 μmol/l by bioluminescence and 4.49 ± 3.27 μmol/l by the spectrophotometr

ISSN:0884-3996    

DOI:10.1002/bio.1170060408

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThis meeting (Chairman, W. R. G. Baeyens) was held on 27—31 May 1991 at the State University of Ghent, Belgium. Abstracts of papers and posters from this meeting on the topics of bioluminescence and chemiluminescence are reproduced in the following sections. The abstracts have been classified under the main topic headings used in the ‘Bioluminescence and Chemiluminescence Literature’ section of this journal, and are listed alphabetically by first a

ISSN:0884-3996    

DOI:10.1002/bio.1170060409

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170060410

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170060411

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY