摘要:

AbstractThis survey update was compiled in June 1993 and includes products not covered in the luminometer survey (Jan 1992; Stanley, PE. J Biolumin Chemilumin 1992; 7:77–108 and 7:157–69) and kit and reagent survey (Nov 1992; Stanley, PE. J Biolumin Chemilumin 1993; 8:51–63). Some products are new whilst for others details have only recently become available to the author. Technical details are provided together with company address and contact inform

ISSN:0884-3996    

DOI:10.1002/bio.1170080502

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractWe have developed a chemiluminescent flow injection method for analysis of bile acid, glucose and ATP using the chemiluminescent assay of NADH using 1‐methoxy‐5‐methylphenazinium methyl sulphate (1‐MPMS)/isoluminol(IL)/microperoxidase (m‐POD) system and immobilized enzyme reactors such as 3α‐hydroxysteroid dehydrogenase, glucosedehydrogenase, hexokinase and glucose‐6‐phosphate dehydrogenase. The standard curves were obtained in the range of 5 ∼ 100 pmol for bile acid, 0.5 ∼ 5.0 nmol for glucose and 10−7∼ 10−5mol/L for ATP. The coefficient of variation for each assay was not more than 4.1% for bile acid, 2.3% for glucose and

ISSN:0884-3996    

DOI:10.1002/bio.1170080503

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractAlthough beta‐agonists remain an important aspect of the treatment of asthma, their role has recently been questioned. Salmeterol has recently been developed as a betaagonist with prolonged bronchodilator action. Using lucigenin‐enhanced chemiluminescence, we have shown that salmeterol inhibits this aspect of phagocyte functionin vitroin a concentration‐dependent manner. However, salmeterol differs from classical beta2‐agonists in that at concentrations between 10−5and 10−3mol/L, its effects on phagocytes cannot be completely reversed by washing the cells or by propanolol. The effects on phagocytes may not therefore be explicable on the basis of betaadrenergic mecha

ISSN:0884-3996    

DOI:10.1002/bio.1170080504

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractWe investigated the effect of prostaglandin E1 on human polymorphonuclear leukocytes,in vivo. Polymorphonuclear leukocytes of a prostaglandin E1 and placebo study group were harvested and their function, as production of oxygen‐derived metabolites and adherence to human cultured endothelial cells, was compared. Additionally, data obtained from polymorphonuclear leukocytes of a prostaglandin E1 and placebo group were compared with data obtained from polymorphonuclear leukocytes from 28 blood donors, who served as a control group.Production of oxygen‐derived metabolites by polymorphonuclear leukocytes during contact with endothelial cells was measured by chemiluminescence. Chemiluminescence was significantly (p<0.01) increased in the placebo group in comparison to the control group decreasing to values of control group after 6 d (post‐trauma). Chemiluminescence response was not significantly suppressed in patients treated with prostaglandin E1 in comparison to the placebo group. Adherence of polymorphonuclear leukocytes (placebo group) to endothelial cells was significantly increased (p<0.01) within the first 6 d post‐trauma Following day 6, values were in the same range as values for the control group. Adherence was not significantly suppressed in patients treated with prostaglandin E1 in comparison to the placebo group. In conclusion, prostaglandin E1 at a dose of 20 ng/kg bw/min does not influence production of oxygenderived metabolites and adherence in polytraumatized patients in comparison to a placebo group. Additionally, production of oxygen‐derived metabolites by polymorphonuclear leukocytes in response to endothelial cells is shown and it is evident that endothelial cells might influence production of oxygen derived metabolites by polymorphonuclear l

ISSN:0884-3996    

DOI:10.1002/bio.1170080505

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstracthtpR−(rpoH, σ32 minus) strain ofE. coliharbouring the wholeluxsystem ofVibrio fischeriis very dim. We have recently shown that GroESL proteins fully recover the expression of theluxsystem in this strain. This work has been undertaken to study our assumption that the GroESL proteins stabilize the LuxR protein, thus enhancing the formation of LuxR–Inducer complex.E. coli htpR−cells harbouring theluxRgene were unable to bind extracellularly added inducer, while late logarithmically growinghtpR+strain bound small quantities of the inducer. Reduction in the nutrient content of the growth medium resulted in a large increase in the capability of these cells to bind the inducer.htpR+orhtpR−E. colistrains harbouring both theluxRand thegroESLgenes bound large quantities of the inducer. The molecular and ecological significance of these results is d

ISSN:0884-3996    

DOI:10.1002/bio.1170080506

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe following references encompass a review of recent literature where thein vivoexpression of eukaryotic and/or prokaryotic luciferases provide for sensitive reporters of cellular activity. The list is subdivided into prokaryotic and eukaryotic applications. We have included the uses of luciferases in elucidating the control of gene expression or for monitoring cell viability. We have not included papers cited by Stanley and Stewart (J Biolumin Chemilumin 1990; 5:141–52) nor have we included papers on the structure and regulation of luciferases as this now substantial literature will be the subject of a future review. References cited in both this review and previous ones are referred to by the number assigned to them in the earlier revie

ISSN:0884-3996    

DOI:10.1002/bio.1170080507

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170080501

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY