摘要:

AbstractThis paper reviews publicaions that combine the technique of flow injection (FI) with chemiluminescence (CL) and bioluminescence (BL) detection, from the earliest papers in 1979/80 to mid‐1992, and refers exclusively to reactions occurring in solution. Airsegmented systems and liquid chromatography with CL detection are not considered unless FI has been used to pre‐optimize the system. The applications have been categorized in terms of the type of CL reaction; there are separate entries for luminol, peroxyoxalate, other CL reactions and BL reactions. Each of the four sections includes a table of applications that lists the analyte, the nature of the reaction, the sample matrix and the limit of detect

ISSN:0884-3996    

DOI:10.1002/bio.1170080402

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe chemical structure of two luciferin precursors PS‐A and PS‐B, isolated from the luminous mushroomPanellus stipticus, were determined as 1‐O‐decanoylpanal (2) and 1‐O‐dodecanoylpanal (3), respectively. Both PS‐A and PS‐B were converted into chemiluminescent luciferins by treatment with 50 mmol/l methylamine in a pH 3.5 buffer solution containing an anionic surfactant Tergitol 4 at 25–35ºC. The luciferins emitted chemiluminescence in a pH 7–8 buffer solution containing a cationic surfactant in the pr

ISSN:0884-3996    

DOI:10.1002/bio.1170080403

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractLeukocyte oxidative function was investigated in a more physiological milieu than currently used in the chemiluminescence (CL) technique. Heparinized blood was mixed with 6% dextran‐T70 (9:1) and the leukocyte‐rich plasma obtained without centrifugation was used for the CL experiments (phagocyte count was adjusted to 0.7 × 106/mL with Hanks' buffer) (method A). In this medium, phagocytes responded to stimulation by opsonized zymosan, producing strong luminescence in the presence of 0.5 m̈ mol/L MCLA. CL was inhibited by superoxide dismutase, suggesting that the luminescence reaction was attributable to O 2−. Granulocytes were also prepared by the usual method involving centrifugation and were then suspended in plasma (method B). Oxidative function of phagocytes prepared by the two methods was studied together with whole blood as aliquots diluted with Hanks' buffer up to a factor of 1000. Luminescence reached a peak value at a dilution factor of 16, but at very high dilutions luminescence decreased sharply. Significantly higher luminescence values were obtained with samples from method A. Luminescence of whole blood peaked at a dilution factor of 248 but it was less than the value obtained using samples prepared by method A or B. As samples prepared by method A contain all the leukocyte populations, platelets, residual red cells and plasma proteins, the assay of leukocyte‐generated reactive oxygens using CL is attained in more physiological conditions than method B in which leukocytes may be damaged owing to repeated centrifugation and hypo

ISSN:0884-3996    

DOI:10.1002/bio.1170080404

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170080405

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170080401

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY