摘要:

AbstractThis survey was compiled in January and February 1992 from information available in public domain literature requested by and supplied to the author by numerous companies in the previous two months. More than 90 luminometers (manual, automatic, microtitre plate, HPLC, LC, GLC, imaging and specials) from more than 60 companies are included. Each company was invited to supply company brochures, technical details, user manual and information about software and any other information concerning their product(s). The response varied from a single information sheet to promotional material and up to full product information and specification with technical details, user manuals and scientific publications. Where an instrument is dedicated to a single task the company may have only provided details relevant to accomplishing that task. Part 2 of this survey will contain photographs of some of the luminometers. It is intended that updates to this review will be published at least annually in this journal and suppliers are invited to provide full technical details of new luminometric equipment to the author.

ISSN:0884-3996    

DOI:10.1002/bio.1170070202

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractTwo strains ofLegionella pneumophilaof different virulence were examined for their influence on the metabolic oxidative activity of human polymorphonuclear leukocytes. The leukocytes exhibited decreased rates of oxygen consumption and diminished chemiluminescence activity following phagocytosis of a virulent strain ofL. pneumophilaserogroup 1. In contrast, phagocytosis of its multipassaged derivative rendered avirulent, was accompanied by increased rates of both oxygen consumption and chemiluminescence activity. Although no differences were observed in oxygen uptake induced by the virulent legionellae compared to leukocytes at rest, statistically significant differences were observed in the chemiluminescence responses. These observations were not unexpected, since the luminol‐enhanced chemiluminescence assay, is more sensitive than the oxygen uptake assay. In spite of decreased metabolic activity of PMN in the presence of virulent legionellae, electron microscope studies showed higher numbers of intracellularL. pneumophilathan the avirulent subtype. Thus, virulent and avirulentL. pneumophilacan be differentiated on the basis of oxygen consumption and chemiluminescence assay

ISSN:0884-3996    

DOI:10.1002/bio.1170070203

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractA novel quantitative nonlabel immunoassay is described. It is based on the recognition of antigen‐antibody complexes by the Fc‐receptors of phagocytic leukocytes and the subsequent activation of these cells. Activation which is proportional to the amount of immune complexes present can be detected by measuring the intensity of chemiluminescence emitted by the activated cells. In addition to determinations of an antigen and an antibody, the binding capacity of complement to antigen‐antibody complexes can be esti

ISSN:0884-3996    

DOI:10.1002/bio.1170070204

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractLuminol chemiluminescence was used to evaluate the scavenging of superoxide, hydroxyl and alkoxy radicals by four antioxidants: dipyridamole, diethyldithiocarbamic acid, (+)catechin, and ascorbic acid. Different concentrations of these compounds were compared with well‐known oxygen radical scavengers in their capacity to inhibit the chemiluminescence produced in the reaction between luminol and specific oxygen radicals.Hydroxyl radicals were generated using the Fenton reaction and these produced chemiluminescence which was inhibited by diethyldithiocarbamate. Alkoxy radicals were generated using the reaction of tert‐butyl hydroperoxide and ferrous ion and produced chemiluminescence which was inhibited equally by all of the compounds tested. For the determination of superoxide scavengers we describe a new, simple, economic, and rapid chemiluminescence method consisting of the reaction between luminol and horseradish peroxidase (HRP). With this method it was found that 40 nmol/l dipyridamole, 0.18 μmol/l ascorbic acid, 0.23 μmol/l (+)catechin, and 3 μmol/l diethyldithiocarbamic acid are equivalent to 3.9 ng/ml superoxide dismutase (specific scavenger of superoxide) in causing the same degree of chemiluminescence inhibition.These results not only indicated that the antioxidative properties of these compounds showed different degrees of effectiveness against a particular radical but also that they may exert their action against more than one r

ISSN:0884-3996    

DOI:10.1002/bio.1170070205

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170070206

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170070207

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170070201

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY