摘要:

AbstractWe developed a sensitive chemiluminescent sandwich‐type enzyme immunoassay (CLEIA) of alpha‐fetoprotein (AFP) using b̃‐D‐galactosidase (b̃‐gal) as a label and 5‐bromo‐4‐chloro‐3‐indolyl‐b̃‐D‐galactopyranoside as a substrate. The CL‐EIA for AFP was performed using two monoclonal antibodies, one antibody is labelled with b̃‐gal, the other is coated onto the inside surface of a polystyrene tube. The detection limit for AFP was 0.5 ng/mL, equivalent to 10 pg/assay tube. The coefficient of variation for within and between assay imprecision were 2.0%−4.9% (n= 10) and 4.4%−9.8% (n= 5), respectively. AFP values in serum determined by this method correlated well with those obtained by radioimmunoassay (n= 26,r= 0.99). This sensitive AFP assay can be performed within 4 h and can be used as

ISSN:0884-3996    

DOI:10.1002/bio.1170080302

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractHigh levels of constitutive aldehyde‐dependent light emission were obtained from non‐growing cells ofLactococcus lactissubsp.diacetylactisF712 transformed withluxA/B when they were suspended in buffered solutions. Inductions of light emission was time‐dependent and was not due to growth, synthesis of luciferase or stimulation of metabolism by fermentable carbohydrate. The major factor controlling light emission in such cells appears to be the intracellular pH value. Experiments with ionophores indicated that a transmembrane pH gradient was not essential for light emi

ISSN:0884-3996    

DOI:10.1002/bio.1170080303

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractIonophores (carbonyl cyanidem‐chlorophenylhydrazone; valinomycin; and the hopderived compounds colupulone,trans‐isohumulone andtrans‐humulinic acid) reduced the rate of dodecanal‐dependent light emission fromluxA/B‐transformed cells ofLactococcus lactissubsp.diacetylactisF712 when the cells were suspended in a buffered medium (pH 6.4) containing glucose. This allowed an assay for ionophores to be devised and permitted measurement of the effects of such compounds on the test organism by the use of a non‐destructive technique i

ISSN:0884-3996    

DOI:10.1002/bio.1170080304

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe chemiluminescence of peripheral blood monocytes and alveolar macrophages was determined in the presence of luminol and lucigenin, either before or after the cell adherence to the luminometer curvettes. In the case of monocytes, cell adherence induces an increase of luminol‐dependent chemiluminescence and has almost no effect on the lucigenin‐dependent chemiluminescence. However, it shows a strong inhibition of the lucigenin‐dependent chemiluminescence and almost no effect on luminol‐dependent chemiluminescence, in the case of alveolar macrophages. These results show that adhesion to plastic alters the metabolic burst of both monocytes and alveolar macrophages. Although the mechanisms are poorly understood, they seem to be related to the modifications that take place during the differentiation of peripheral monocytes to alveolar macr

ISSN:0884-3996    

DOI:10.1002/bio.1170080305

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractStimulation of phagocytes by several cytokines causes superoxide generation and consequently chemiluminescence. Since antigen‐activated lymphocytes generate cytokines, we investigated whether antigen recognition by mononuclear cells, which contain both lymphocytes and monocytes, is accompanied by changes in lucigenin‐dependent chemiluminescence. Mononulcear cells which underwent antigen‐induced proliferation showed a delayed rise in lucigenin‐dependent chemiluminescence in the absence of other stimuli. The common recall antigenCandida albicansincreased spontaneous chemiluminescence of mononuclear cells from unselected donors up to 20‐fold over control values after 48–72h of culture. With Rabies virus vaccine as specific antigenic stimulus, only mononuclear cells from rabies immunized individuals responded with enhanced delayed chemiluminescence. In contrast to opsonized zymosan and phorbol myristate acetate, antigens induced no oxidative burst within one hour after addition. Delayed mononuclear cel chemiluminescence was inhibited by the superoxide scavenger superoxide dismutase and by di‐phenylene iodonium, a selective inhibitor of the phagocyte NADPH oxidase. A neutralizing monoclonal antibody against interferon‐gamma completely abrogated antigen‐induced chemiluminescence. Recombinant interferon‐gamma by itself induced delayed mononuclear cell chemiluminescence. Thus, antigen‐induced delayed mononuclear cell chemiluminescence represents activation of phagocyte NADPH oxidase by interferon‐gamma generated b

ISSN:0884-3996    

DOI:10.1002/bio.1170080306

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170080307

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170080301

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY