摘要:

AbstractA solid‐phase enzyme immunoassay method using goat anti‐CRP sensitized to polyvinyl 96 well plates and a second rabbit anti‐CRP highly specific for gamma mobility CRP is described in this study. Examination of serum samples of normal healthy individuals from cord blood, children (2–12 yrs) and adults (16–83 yrs) by this EIA method showed a progressive increase in CRP levels with age. The mean and standard error of the mean in ng/ml CRP was as follows with respect to age distribution: cord blood, 310 ± 54; children (2–12 yrs), 664 ± 87; adults (16–35 yrs), 1,145 ± 116; adults (36–50 yrs), 3,775 ± 605; adults (51–83 yrs), 7,718 ± 1,482. Individuals in the age range of 22–34 yrs with one or more abnormality in the chemistry profile analysis showed CRP levels with a higher x̄ ± S.E.M. (3,615 ± 925). This is in contrast to the normal individuals of the same age range (1,145 ± 116). This EIA method is sensitive, reproducible, and specific. It should be a useful quantitative method for monitoring low level CRP responders such as

ISSN:0887-8013    

DOI:10.1002/jcla.1860010122

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractPrimary infection with Epstein‐Barr virus (EBV) and reactivation of latent virus are associated with increased antibody titers against the diffuse early antigen (EA‐D). In order to better define the antigenic epitopes recognized by antibodies from patients with infectious mononucleosis (IM) and with other disease states, a series of synthetic peptides were prepared based on the DNA sequence encoding the EA‐D molecule. One synthetic peptide (K7b) was reactive with the majority of sera from patients with acute IM. Anti‐K7b activity was most readily detected among IgM and IgA antibodies and to a lesser extent among IgG antibodies. In contrast, significant elevations of anti‐K7b activity were observed in less than 5% of healthy adults. Serial analysis of samples from individuals prior to and after exposure to EBV demonstrated increased anti‐K7b reactivity associated with the symptoms of acute IM. Elevated anti‐peptide K7b titers also were found in sera of patients with nasopharyngeal carcinoma and with Sjogren's syndrome (an autoimmune disease involving the salivary glands). Four different synthetic peptides from other regions of the EA‐D molecule were not reactive with antibodies from these patients nor from IM patients. These results suggest that peptide K7b defines an antigenic epitope recognized during primary EBV infection and during viral reactivation occurring in patients with autoimmune and neo

ISSN:0887-8013    

DOI:10.1002/jcla.1860010123

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

ISSN:0887-8013    

DOI:10.1002/jcla.1860010124

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

ISSN:0887-8013    

DOI:10.1002/jcla.1860010101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY