摘要:

AbstractStudies on embryonic development in vitro as well as observations in vivo, suggested that two aspects of oviduct physiology are important for early development. On one side has to be considered the oviduct “environment”: temperature, pH, osmotic pressure, nutrients, oxygen tension, free radical scavengers, etc. On the other, the oviduct “active components”: stimulatory and/or regulatory molecules, supposed to finely regulate the fertilisation process and the first differentiative steps.While the physical environment of the oviduct has been under investigation for some decades, studies on oviduct‐specific molecules and their functions have only been developed much more recently. The amount of information on this topic, however, has rapidly reached the size that demands a summary.In this review the descriptive literature on oviduct specific proteins will be examined as a basis for illustrating the possible functions of these molecules. In particular their role in fertilisation and early embryonic cleavages will be analysed in some details. Finally a section is devoted to the presence and physiological significance of growth factors in oviduct fluid. © 1995 Wiley

ISSN:1059-910X    

DOI:10.1002/jemt.1070320102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractOviductal epithelium of the baboon,Papio cynocephalus, was studied utilizing light, scanning, and transmission electron microscopy. Results of counts made of nonciliated, ciliated, and ciliogenic cells were analyzed statistically. The percentages of nonciliated cells of the fimbria and ampulla during the early proliferative and late secretory stages of the menstrual cycle were significantly greater than those during the mid‐proliferative and late proliferative‐early secretory stages, due to deciliation. This paper emphasizes previously unreported apical surface morphology as viewed by scanning and transmission electron microscopy. The microvillar pattern of the fimbrial secretory cells differs from that of the ampullar and isthmic cells in that the microvilli originate from thick apical protrusions and vary greatly in length and number as related to the cycle. A ridge demarcating the apical intercellular junction is composed of rows of microvilli during the early proliferative and late secretory stages. During the early proliferative and late secretory stages an increased degree of invagination of the basal and lateral plasma membranes occurs as the height and width of the cells decreases. The general numbers and distribution of the organelles of the various types of oviductal cells agree with that described for the ampulla and isthmus by Verhage et al. [(1990) Am. J. Anat., 187:81–90]; however, fimbrial epithelium was not included in that study. Other cyclic ultrastructural changes not examined previously include variation in the number of lipid droplets and their location, and in the number and relationships of glycogen particles to other structures. © 1995 Wiley‐L

ISSN:1059-910X    

DOI:10.1002/jemt.1070320103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractThe mucous surfaces of the oviducts of mammals, especially humans, are dependant on estradiol. The mucus glycoproteins and glycocalyceal glycoproteins have, however, barely been studied. Biochemical analyses have focussed on the relatively low molecular weight glycoproteins likely to be found in more serous‐type granules of the ampulla and not on the very high MW glycoproteins typical of mucus and represented in the isthmus by morphological evidence of mucus secretion. Quantitatively, secretion from the ampulla is likely to predominate, because of its huge surface area compared with the isthmus. But functional closure of the isthmus under the influence of estradiol in the absence of progesterone means that it is the isthmus where luminal secretions accumulate—and where mucus glycoproteins will exert their most important effects on spermatozoa ascending the reproductive tract, and then on fertilized ova en route to the uterus. Further study of the extracellular, intraluminal, carbohydrate‐rich environment of the oviductal isthmus, especially in humans, is likely to prove rewarding. Sampling of these secretions is now feasible using transvaginal, transuterine fallopian tube catheters that are in clinical use. © 1995 Wiley‐L

ISSN:1059-910X    

DOI:10.1002/jemt.1070320104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

ISSN:1059-910X    

DOI:10.1002/jemt.1070320105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractThe major objective of this study was to examine the hormonal regulation of a human oviduct‐specific glycoprotein (huOGP) throughout the menstrual cycle and in all regions of the human oviduct. Regulation of synthesis and secretion was examined at both the protein (Western immunoblots and immunocytochemistry) and mRNA (Northern and slot blots) levels and correlated with changes in the morphological features of the oviductal epithelial cells throughout the cycle. Immunoblot analysis of oviductal fluid and explant culture media from all regions of the oviduct demonstrated that huOGP is primarily found during the follicular stage of the cycle and is not present in serum, follicular fluid, or uterine endometrium. Moreover, two‐dimensional (2‐D) immunoblots showed that all major isoelectric variants of huOGP observed on 2‐D fluorographs are immunologically related. Light microscopic immunocytochemistry localized huOGP to oviductal secretory cells in both ampulla and isthmic regions, with the most intense immunoperoxidase staining seen in midcycle samples. Using an indirect immunogold technique at the electron microscopic level, huOGP was specifically localized to secretory granules of the ampullary and isthmic nonciliated epithelial cells. The ultrastructural characteristics of these secretory cells during the mid to late follicular phase of the cycle suggested elevated protein synthetic activity. In addition, mRNA expression for huOGP was elevated in all regions of the oviduct in midcycle specimens. Collectively, these data indicate that huOGP is a major tissue‐specific, stage‐specific secretory product of the human oviduct during the periovulatory stage of the cycle and support the hypothesis that huOGP synthesis and secretion may be regulated by fluctuations in the levels of estrogen and progesterone. © 1995 Wil

ISSN:1059-910X    

DOI:10.1002/jemt.1070320106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractA two‐stage jet polishing technique is described which, utilising the effects of the characteristic current‐voltage behaviour of electropolishing solutions, can produce excellent TEM foils of relatively coarse two‐phase materials. © 1995 Wiley‐L

ISSN:1059-910X    

DOI:10.1002/jemt.1070320107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

ISSN:1059-910X    

DOI:10.1002/jemt.1070320108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

ISSN:1059-910X    

DOI:10.1002/jemt.1070320101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY