ISSN:1059-910X    

DOI:10.1002/jemt.1070310602

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractIn several mammalian species, the epithelial secretory cells of the oviduct synthesize and secrete specific glycoproteins that become associated with the zona pellucida of the ovulated oocyte. These glycoproteins are collectively designated as oviductins. A monoclonal antibody directed against hamster oviductin was used to study the ontogeny of this glycoprotein. Indirect immunofluorescence experiments performed on sections of hamster oviduct revealed that the glycoprotein begins to be secreted in 10‐day‐old females and that all of the oviductal secretory cells showed fluorescent staining by day 14. The intensity of the immunofluorescence reaction reached a maximum in the 28‐day‐old females. The oviducts of the 7‐day‐old hamster incorporated [35S]methionine in vitro into several proteins; however, the production and secretion of detectable amounts of radiolabeled oviductin only began at 14 days of age and reached a maximum at day 28 of age. It appears that the ontogeny of oviductin parallels the hormone dependent changes leading to sexual maturation and that its maximum secretion is already established at the time of the first ovulatory cycle. These results are substantiated by the fact that the production of oviductin is induced in estradiol‐treated, but not progesterone or non‐treated prepubertal animals, as determined by indirect immunofluorescence experiments. © 1995

ISSN:1059-910X    

DOI:10.1002/jemt.1070310603

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractThe hamster oviduct secretes a high molecular weight antigen that belongs to the family of glycoproteins known as oviductins. In the present study, using immuno‐electron microscopy, we examined the location of this hamster oviductin‐1 (Hm Ov‐1) in hamster oviductal oocytes and early embryos up to the blastocyst stage. The immunoreactive pattern of Hm Ov‐1 changes markedly during the embryo development. In oviductal oocytes prior to fertilization, Hm Ov‐1 was associated exclusively with the zona pellucida. Following fertilization, immunolabeling was detected in the perivitelline space and over the plasma membrane of 2‐cell, 4‐cell, and 8‐cell embryos as well as young blastocysts. The change of the immunoreactive pattern was accompanied by the formation of an abundant number of coated pits, endocytic vesicles, multivesicular bodies, and lysosomal‐like structures which were strongly labeled by gold particles. These immunogold‐labeled cytoplasmic organelles characteristic of the endosomal‐lysosomal apparatus were particularly evident in 2‐cell, 4‐cell, and 8‐cell embryos and showed a decrease in number in the blastocysts. The close resemblence between the labeled flocculent material detected in the perivitelline space and that found in the zona matrix of early embryos and blastocysts suggested that the Hm Ov‐1‐associated electron‐dense, flocculent material in the perivitelline space originated from the zona pellucida and was later endocytosed by the blastomeres through coated pits and endocytic vesicles. The detection of Hm Ov‐1 in numerous multivesicular bodies and lysosomal structures indicated that the oviductin is eventually degraded. Although the exact functional role of Hm Ov‐1 is not known, the presence of a copious amount of Hm Ov‐1 in early hamster embryos may be ascribed to a special relationship between this particular oviductin and emb

ISSN:1059-910X    

DOI:10.1002/jemt.1070310604

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractLocalization of individual glycosidic residues and sialic acid acceptor sugars was investigated by conjugated lectins in the rabbit oviduct under physiological hormonal conditions and human chorionic gonadotropin (HCG) administration. Ampulla and isthmus were found to exhibit lectin binding profiles typical of each hormonal stage. Two different sialylated glycomolecules were identified within the epithelial lining; in particular, sialoglycoconjugates characterized by the terminal sequence sialic acid‐galactose were visualized in the secretory cells and the sialic acid‐N‐acetylgalactosamine terminal disaccharides were localized on both ciliated and secretory cells of the entire oviduct. Surface and cytoplasmic sialoglycoconjugates were also found to exhibit a differential behaviour inside the two oviduct tracts examined. Present findings further supported the idea that in ampulla and isthmus, the greatest modifications consequent to hormone treatment take place at different times. © 1995 Wiley‐L

ISSN:1059-910X    

DOI:10.1002/jemt.1070310605

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractThe cyclic fluctuations in circulating levels of 17β‐estradiol and progesterone that occur during the menstrual or estrous cycle are responsible for dramatic, cyclic changes in the epithelial lining and secretory status of the mammalian oviduct. The timely transition in the synthesis and release of oviduct proteins, due to the ovarian steroids, and their interactions with oocytes, sperm, and the fertilized ovum underscore key biological events during gamete interactions and early embryonic cleavage. The regulation of these secretory alterations during the first few days of pregnancy is discussed with respect to the influence of the ovarian steroids, their interactions with the embryo microenvironment, and the possible ways in which they may mediate the critical reproductive events of fertilization and embryo development. © 1995 Wiley‐Liss

ISSN:1059-910X    

DOI:10.1002/jemt.1070310606

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractThis report describes an easy method of isolation and cell culture of the epithelial cells of cow oviduct. Incubation of cow oviduct with 0.1 mg/ml collagenase in the lumen for 90 minutes helped to dislodge large numbers of ciliated and secretory epithelial cells. The isolated cells, when seeded on plastic, proliferated very quickly and became confluent in 8–10 days in 35 mm Petri dishes. The isolated ciliated cells which attached to the plastic dish lost their cilia after 4–5 days in culture. The cultured epithelial cells were keretin positive. The isolated bovine oviduct epithelial cells, when cultured on plastic precoated with 10 mg/ml matrigel, organized themselves into hollow tubes or spheres with microvilli directed towards the lumen. The epithelial cells seeded on 2 mg/ml matrigel became subconfluent in 15–20 days after seeding. The histoarchitecture of the secretory cells growing in vitro on matrigel resembled that of intact oviduct secretory epithelial cells. Occasional ciliated cells containing large number of mitochondria were observed in the monolayer cultured on 2 mg/ml matrigel substratum but possessed few cilia. The oviduct epithelial cells cultured on 2 mg/ml matrigel incorporated35S‐methionine linearily into protein up to 8 hours in the presence of estradiol or progesterone. The fluorograph of the newly synthesized proteins indicated the presence of an additional 60 kd protein in the cell extract of epithelial cells incubated with estradiol. © 1995 Wiley

ISSN:1059-910X    

DOI:10.1002/jemt.1070310607

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

摘要:

AbstractThe oviduct (uterine tube) plays a major role in reproduction. It is a dynamic organ which selectively permits a few sperm to undergo capacitation and reach the oocyte which has continued to undergo maturation following ovulation. Then following fertilization the embryo undergoes cleavage before arriving in the uterus. Extensive information has become available from in vitro studies on oocytes as well as spermatozoal interactions with oviductal cells. Bovine oviduct epithelial cell (BOEC) monolayers with simple media provide an environment in which zygotes can be cultured to blastocysts in 6 days with cell numbers essentially equivalent to blastocysts grown totally in the donor animal. These yield normal pregnancy rates upon transfer. The simple protein‐free media currently under test hold promise for elucidating specific requirements of the preimplantation embryo and these defined conditions facilitate many related studies on in vitro fertilization and genetic engineering of embryos.The second part of this paper is an extensive study on the interaction of fresh and frozen‐thawed bull spermatozoa with BOEC and segments of intact oviducts as viewed by SEM. Both types of oviductal cells were incubated at 39°C for 0, 3, 6, and 9 hours, using material obtained from periovulatory cows. Sperm attached immediately to both types of epithelium and reached a peak at 3 hours. They were found primarily in the furrows of the intact oviducts. Secretory droplets appeared rapidly on the anterior portion of the sperm head and acrosomal changes were evident in 3 hours, similar to those reported in vivo. Changes were more rapid with frozen‐thawed sperm. © 1995 Wiley‐

ISSN:1059-910X    

DOI:10.1002/jemt.1070310608

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

ISSN:1059-910X    

DOI:10.1002/jemt.1070310609

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY

ISSN:1059-910X    

DOI:10.1002/jemt.1070310601

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1995

数据来源: WILEY