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1. |
Serum Thyroxine Binding Capacity-Dependent Bias in an Automated Free Thyroxine Assay |
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Journal of Immunoassay,
Volume 20,
Issue 4,
1999,
Page 201-221
N.D. Christofides,
E. Wilkinson,
M. Stoddart,
D.C. Ray,
G.J. Beckett,
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摘要:
The magnitude of serum thyroxine (T4) binding capacity (sBC) dependent bias in the AXSYM free thyroxine (FT4) assay was assessed using two recently described tests. One of the tests uses a direct equilibrium dialysis (ED) FT4 assay as the reference method. the results obtained with the AXSYM method were compared with those obtained by the ED FT4 method in patient sera having a wide range of sBC. the other test involves comparison of the FT4 results obtained following dilution of sera by an inert buffer, to theoretically derived FT4 results. As serum dilution causes a predictable decrease in sBC, the demonstration of a negative bias whose magnitude increases in parallel to the dilution, is indicative of an sBC-dependent bias. the AXSYM FT4 assay exhibited a significant sBC-dependent bias. This sBC-dependent bias is likely to have been caused by the presence of significant amounts of T4 binding proteins in the assay reagents.
ISSN:0197-1522
DOI:10.1080/01971529909349351
出版商:Taylor & Francis Group
年代:1999
数据来源: Taylor
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2. |
Monoclonal Antibody-Based Sensitive Enzyme-Linked Immunosorbent Assay for Murine Serum Amyloid A |
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Journal of Immunoassay,
Volume 20,
Issue 4,
1999,
Page 223-235
Toshiyuki Yamada,
Takeaki Fukuda,
Atsufumi Wada,
Yoshihisa Itoh,
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摘要:
Enzyme-linked immunosorbent assay (ELISA) methods for measuring murine serum amyloid A (SAA), a representative acute phase reactant, were developed utilizing a newly produced monoclonal antibody. Two site-ELISA, in which the monoclonal antibody was used as the captured antibody, was sensitive enough to determine the SAA concentration in mice at the steady state. Direct binding ELISA, in which the sample SAA bound to the plastic wells was detected by the antibody, was simple and suitable for measuring the elevated SAA, but could not analyze the resting level of SAA because of the need for high dilution in plasma samples. Plasma SAA concentrations were measured in ten ICR mice on the day of purchase and at the end of seven days of ordinary rearing. the SAA concentration of one animal decreased from 1.6 to 0.5 mg/1 during a week, while the others had no obvious changes. the plasma SAA of the ten animals after one week of rearing ranged from 0.3 to 0.8 mg/1 with a mean of 0.47. These mice, two days after 10 μ g lipopolysaccharide were given, had increased SAA values up to a mean of 300 mg/1, though with variations between animals.
ISSN:0197-1522
DOI:10.1080/01971529909349352
出版商:Taylor & Francis Group
年代:1999
数据来源: Taylor
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3. |
Validation of an Elisa for the Quantitation of Lanoteplase, A Novel Plasminogen Activator |
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Journal of Immunoassay,
Volume 20,
Issue 4,
1999,
Page 237-252
B. Stouffer,
S. Habte,
N. Vachharajani,
L. Tay,
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摘要:
An ELISA was developed and validated for the quantitation of lanoteplase in human citrated plasma. the ELISA employed a monoclonal anti-lanoteplase antibody absorbed onto 96-well microtiter plates to capture lanoteplase in citrated human plasma samples containing PPACK, a protease inhibitor. the captured lanoteplase was detected using a biotinylated rabbit anti-lanoteplase polyclonal antibody. the standard curve range in human plasma for the ELISA was 7-100 ng/ml.
ISSN:0197-1522
DOI:10.1080/01971529909349353
出版商:Taylor & Francis Group
年代:1999
数据来源: Taylor
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4. |
Evaluation of a new Troponin I Method on the Bayer Immuno 1™ Immunoassay Analyser |
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Journal of Immunoassay,
Volume 20,
Issue 4,
1999,
Page 253-273
GeraldineH. Clark,
SimonR. O. Kennon,
ChristopherP. Price,
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摘要:
We have evaluated the analytical and clinical performance of an automated immunoassay for serum cardiac troponin I (Bayer Immuno 1™, Bayer Diagnostics, Tarrytown, NY). the between batch imprecision was found to be between 1.2 and 3.2% over the concentration range 2.5-34.0 μg/L. the analytical range obtained from duplicate analysis of patient samples and defined as a coefficient of variation of 10% or less was 0.3 - 200 μg/L. the detection limit was found to be less than 0.1 μg/L. A method comparison with the Dade Stratus method (Dade Behring, Wilmington, DE) yielded regression statistics with a slope of 0.705 and an intercept of -0.260. an analysis of samples from 40 patients with renal failure demonstrated six with detectable levels of troponin I (0.2-1.9 μg/L). Samples from patients with paraproteinaemia did not demonstrate detectable troponin I (from n = 30); however, two patients with elevated rheumatoid factor titers (from n = 20) demonstrated a detectable amount of troponin I (0.1 and 0.2 μg/L). in a study of 100 patients admitted with acute chest pain and a diagnosis of unstable angina, 6 were subsequently diagnosed as having suffered a myocardial infarction. on admission the sensitivity and specificity of the troponin I results were 26.7% and 94.7%, respectively, moving to 100% and 83% 12 hours after admission.
ISSN:0197-1522
DOI:10.1080/01971529909349354
出版商:Taylor & Francis Group
年代:1999
数据来源: Taylor
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5. |
Acknowledgement |
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Journal of Immunoassay,
Volume 20,
Issue 4,
1999,
Page 275-275
RobertH. Christenson,
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ISSN:0197-1522
DOI:10.1080/01971529909349355
出版商:Taylor & Francis Group
年代:1999
数据来源: Taylor
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6. |
Author Index to Volume 20 |
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Journal of Immunoassay,
Volume 20,
Issue 4,
1999,
Page 277-278
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PDF (37KB)
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ISSN:0197-1522
DOI:10.1080/01971529909349356
出版商:Taylor & Francis Group
年代:1999
数据来源: Taylor
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7. |
Subject Index to Volume 20 |
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Journal of Immunoassay,
Volume 20,
Issue 4,
1999,
Page 279-281
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PDF (102KB)
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ISSN:0197-1522
DOI:10.1080/01971529909349357
出版商:Taylor & Francis Group
年代:1999
数据来源: Taylor
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