摘要:

Laser light scattering immunoassay (LIA) was proposed as a prospective diagnostic method for the detection of antibody (or antigen) by monitoring the agglutination of antigen (or antibody) coated carrier particles using dynamic light scattering (DLS) as probe, LIA is a very sensitive assay as it can detect microscopic immune complexes even when antibody (or antigen) level is low. A sizeable number of human sera collected from malaria endemic areas and hospitals have been analysed by ELISA using Pf parasite lysate or a RESA derived synthetic peptide as antigen parallel to LIA using Pf antigen coated polystyrene latex beads. Comparative analysis of data suggests LIA to be as good as ELISA and possibly better in terms of sensitivity and simplicity. LIA can be a simple and inexpensive immunoassay suitable for field use and mass application.

ISSN:0197-1522    

DOI:10.1080/01971529909349346

出版商:Taylor & Francis Group    

年代:1999

数据来源: Taylor

摘要:

A homogeneous fluorescence polarization assay (FPIA) for detection of bovine antibody toBrucella abortuswas validated in Argentina. Sera were defined based on their reactivity in the buffered antigen plate agglutination test (BPAT) and the competitive enzyme immunoassay (CELISA). Sera negative in these tests were collected from farms without evidence of brucellosis (n=733). Sera positive in the two tests were collected from cattle on farms from whichB. abortuswas isolated from at least one animal (n=1039). Sera from cattle vaccinated 26, 89, 240 and 272 days previously withB. abortusstrain 19 were collected and tested. A cut-off value of 87 mP was determined for the FPIA, resulting in relative sensitivity and specificity values of 98.1 and 99.6%. the specificity forB. abortusstrain 19 vaccinated cattle was 64.9% (26 days post vaccination, DPV), 92.1% (89 DPV), 98.6% (242 DPV) and 97.1% (272 DPV). These values were compared to those obtained with the BPAT, the CELISA, the indirect ELISA, the complement fixation test and the 2-mercaptoethanol agglutination test. Sera from 18 cattle which were vaccinated and revaccinated withB. abortusstrain 19 were also tested by the same assays and the FPIA was found to be 100% specific. the use of the FPIA as a diagnostic test for brucellosis is discussed.

ISSN:0197-1522    

DOI:10.1080/01971529909349347

出版商:Taylor & Francis Group    

年代:1999

数据来源: Taylor

摘要:

Sustainable management of economically important squid requires monitoring of changes in their abundance, which are relatedinter alia, to their success in the food chain. the highest mortality is expected in the paralarval stages, which are prone to starvation. Causes of starvation may be linked to the lack of suitable prey. A multiple detection system was developed for the simultaneous identification of five putative zooplankton prey in the guts of paralarval Chokka squid,Loligo vulgaris reynaudii, by employing polyclonal rabbit antisera in conjunction with solid phase immunoassays. Specificities of antisera were validated by ELISA screening against different zooplankton taxa. Cross-reactions observed with ELISA were minimized through manipulation of antibody and antigen concentrations resulting in more specific detection of target prey antigens when used in an immunodot assay. Application of this optimised immunoassay detected multiple predation in paralarval squid samples collected from diverse areas in the Agulhas Bank ecosystem on the south coast of South Africa.

ISSN:0197-1522    

DOI:10.1080/01971529909349348

出版商:Taylor & Francis Group    

年代:1999

数据来源: Taylor

摘要:

Horse radish peroxidase, alkaline phaosphatase and β-D-galactosidase are widely used as labels in the development of enzyme immunoassays (EIAs). Enzyme β-lactamase, though introduced as a label in late seventies has not yet become very popular inspite of having the necessary features of an enzyme to be used in EIAs. the present article reviews assays developed with this enzyme, highlights its salient features and brings out an argument in favour of its wide spread use in EIAs.

ISSN:0197-1522    

DOI:10.1080/01971529909349349

出版商:Taylor & Francis Group    

年代:1999

数据来源: Taylor

摘要:

Seven ELISAs were developed by using several combinations of anti-human IL-1β antibodies for detecting interleukin 1β (IL-1β) in cell culture supernatants. These ELISAs have different sensitivities in detecting standard preparations of recombinant human IL-1β (WHO reference standard) compared with conventional preparations of IL-1β produced by stimulated human peripheral blood mononuclear cells. the observed differences were attributed to differences in epitope specificity of the various monoclonal antibodies used and the heterogeneity of IL-1β secreted into culture supernatants. the presence of soluble IL-1 receptor type I did not alter the levels of IL-1β detected by these ELISAs. However, soluble IL-1 receptor type II interfered with the detection of IL-1β to different degrees in these ELISAs. A method involving standarization by means of separate measurement of the amount of receptor and its inhibitory effect in the IL-1β ELISA, yields consistent estimates of the correct IL-1β levels.

ISSN:0197-1522    

DOI:10.1080/01971529909349350

出版商:Taylor & Francis Group    

年代:1999

数据来源: Taylor