摘要:

SummaryThe biochemical properties of ALDH isozymes have been examined in human tissues and one set, designated ALDH3, has been studied in detail. These components occur at highest levels in lung and stomach, but were not expressed in fetal tissues, or in blood, hair roots and fibroblasts. The ALDH3 isozymes show optimal activity with benzaldehyde and can use either NAD or NADP as cofactor. Antiserum against a partially purified ALDH3, from stomach, selectively precipitates this isozyme from human tissues and selectively recognizes an homologous component in the rat. Human and rodent ALDH3 were not immunoprecipitated by anti‐ALDH1 or anti‐ALDH2 antisera. High levels of expression were found in human‐rodent hybrids, constructed using rat hepatoma cells, and these hybrids were used to assign the human ALDH3 gene to chromoso

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01680.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryA cDNA clone complementary to the mRNA encoding human myosin heavy chain has been isolated from a human fetal skeletal muscle cDNA library. A 600 base pair fragment of the inserted human cDNA has been used as probe in the Southern analysis of DNA from panels of rat/human and mouse/human somatic cell hybrids. All the sequences detected by this probe have been mapped to chromosome 17 in the region 17pter → 17p11. There was no evidence for MHC sequences on any other chromosom

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01681.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryAn electrophoretic survey of 509 individuals of Japanese, Mexican American, American Negro, Eskimo, Amerindian and Anglo‐American origin failed to reveal genetically determined variation at the alpha‐keto acid reductase locus by starch gel electrophoresis. Additional screening of 232 individuals by thin layer isoelectric focusing in polyacrylamide gels detected a single phenotype. Interspecific variation at the alpha‐keto acid reductase locus is clearly resolved using either method. These results indicate that the alpha‐keto acid reductase locus is monomorphic in most human population groups despite earlier results suggesting the existence of genetic polym

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01682.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryMethylation of CCGG sites was examined in four regions of the X chromosome with four X‐chromosome clones, three obtained by cloning random segments and one encoding a structural gene. In DNA from human peripheral blood cells unmethylated sites correlating with the inactive X chromosome were detected in the vicinity of two of the random clones and also in the vicinity of a cloned sequence of the X‐linked phosphoglycerate kinase gene (PGK). The third random clone covered a region whose methylation pattern was unchanged between the active and inactive X chromosomes. Differential methylation at the sites detected appears to have no functional role in the maintenance of the inactive X chromosome since both active and inactive X chromosomes were found to be undermethylated in DNA from human lymphoblastoid ce

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01683.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryFamilies segregating forPEPDwere investigated for linkage betweenPEPDandAPOC2.The results provide evidence for close linkage betweenPEPDandAPOC2in males.

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01684.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryChiasma distribution data on chromosomes 1, 2 and 9 from a reciprocal translocation carrier with a 46, XY, t(9; 10) (p22; q24) karyotype were used to calculate genetic distances and recombination fractions for chromosome segments corresponding to the major mitotic bands and for intervals between the centromeres and points at 10% intervals along the chromosome arms. These values were compared with those from control males with normal karyotypes. The translocation showed a marked increase in crossing‐over in one specific region of chromosome 9 and, in addition, there was evidence of interchromosomal effects in chromosomes 1 and

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01685.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryQ‐banded chromosome 1 bivalents from six human males were measured in order to determine the locations of the major band borders. Chiasma position was also recorded in these bivalents in order to determine whether chiasmata preferentially occurred in Q‐bright regions, Q‐dark regions or in the interfaces between. The results indicated that the locations of the major bands of chromosome 1 were very similar at diakinesis and at mitotic prometaphase and that chiasma distribution was not governed by the banding pattern of the chrom

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01686.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryChromosome studies to detect the folate sensitive fragile sites have been carried out on 2439 randomly selected neonates. Four autosomal fragile sites were detected in this group. Similar studies were carried out on referred patients, special school students and sheltered workshop employees. The incidence of fragile X in these groups was 6/1936, 13/502 and 0/128 respectively. Autosomal folate sensitive fragile sites were seen in 14/1936, 5/502 and 2/128 individuals respectively.

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01687.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01688.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0003-4800    

DOI:10.1111/j.1469-1809.1985.tb01689.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY