摘要:

AbstractTwo simple, rapid and sensitive extractive spectrophotometric methods have been developed for the assay of cinnarizine (CNR) in pure and pharmaceutical formulations. The methods are based on the formation of chloroform soluble ion‐association complexes of CNR with thymol blue (TB) and with cresol red (CR) inNaOAc‐AcOH buffer of pH 3.6 for TB and in KCl‐HCl buffer of pH 1.6 for CR with absorption maxima at 405 nm and at 403 nm for TB and CR, respectively. Reaction conditions were optimized to obtain the maximum color intensity. The systems obeyed Beer's law in the range of 0.6–15.8 and 0.8–16.6 μg mL−1for TB and CR, respectively. Various analytical parameters have been evaluated and the results have been validated by stat

ISSN:0009-4536    

DOI:10.1002/jccs.200700011

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

AbstractThe reaction of the hydrazide of pyridine‐4‐acetic acid with isothiocyanate gave thiosemicarbazide derivatives respectively. Further cyclization with 2% NaOH led to the formation of 4‐substituted 3‐(pyridin‐4‐ylmethyl)‐1,2,4‐triazoline‐5‐thione and 3‐(pyridin‐4‐ylmethyl)‐1,2,4‐triazoline‐5‐thione. The structures of all new products were confirmed by ana

ISSN:0009-4536    

DOI:10.1002/jccs.200700012

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

AbstractA new demethylacetovanillochromene glycoside, 2,2‐dimethyl‐2H‐(8‐hydroxy‐6‐acetyl)‐[2,3‐b] pyran‐8‐O‐β‐D‐apiofuranosyl‐(1 →6)‐β‐D‐glucopyranoside (1), together with twenty‐three known compounds, have been isolated from the root bark ofRodgersia sambucifolia.These known compounds include two diterpenoids, three flavonoids, two catechins, four lignans, two benzenoids, two isocoumarins, two steroids and six monoterpene glycosides, which were d

ISSN:0009-4536    

DOI:10.1002/jccs.200700013

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

AbstractIn the present investigation, a series of 5‐(‐4‐(substituted)phenyl)‐3‐(4‐hydroxy‐3‐methylphenyl)‐4,5‐dihydro‐1H‐1‐pyrazolyl‐2‐toluidino methane thione and 5‐(substituted)phenyl‐3‐(4‐hydroxy‐3‐methylphenyl)‐4,5‐dihydro‐1H‐1‐pyrazolyl‐2‐methoxy anilino methane thiones were synthesized and were examined against human lung tumor cell line (A549)in vitrousing the MTT assay system. Among those tested, 5‐(4‐flurophenyl)‐3‐(4‐hydroxy‐3‐methylphenyl)‐4,5‐dihydro‐1H‐1‐pyrazolyl‐2‐toluidino methane thione&5‐(4‐chlorophenyl)‐3‐(4‐hydroxy‐3‐methylphenyl)‐4,5‐dihydro‐1H‐1‐pyrazolyl‐2‐methoxy anilino methane thione

ISSN:0009-4536    

DOI:10.1002/jccs.200700014

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

AbstractThree new apianen lactones Maomailiaoine I (1), Maomailiaoine II (2) and Maoimaliaoine III (3), together with one known apianen lactone, five known flavonoids and three other known compounds were isolated fromPolgonum ciliinerve Ohwi.Their structures were deduced from spectroscopic methods and 2D NMR techniques.

ISSN:0009-4536    

DOI:10.1002/jccs.200700015

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

AbstractCondensation of 1‐phenylpyrazolidine‐3,5‐dione1with 3‐formylchromone afforded 4‐(chromenylmethylene)pyrazolidinedione2, which was reacted with hydrazine or hydroxylamine in different molar ratios and conditions to give the corresponding pyrazole and isoxazole derivatives3‐8, respectively. Compound2was subject to react with ammonia, N,S‐ or S,S‐acetals, mercaptoacetic acid, cyanoacetamide or cyanothioacetamide to give the corresponding pyridine, dithiine, thiazine and thiophene,9‐14, respectively. The reaction of compound2with thiourea, guanidine, cystamine,o‐aminothiophenol, ethylenediamine,o‐phenelenediamine or barbituric acid afforded the corresponding thiazine, pyrimidine, thiazepine, diazepine, and pyran derivatives17‐23, respectively. The study of the reaction of compound2with nucleophilesviachromene ring o

ISSN:0009-4536    

DOI:10.1002/jccs.200700016

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

AbstractThe allenes 1,2,3,4,5‐pentafluoro‐6‐(3‐phenylpropa‐1,2‐dienyl)benzene4, 3‐(3‐phenylpropa‐1,2‐dienyl)pyridine11and 3‐(3‐(pyridine‐3‐yl)propa‐1,2‐dienyl)pyridine17and the acetylenes5,12and16were obtained by reduction of the corresponding propargylic acetates3,10and15by Samarium(II) iodide in the presence of Pd(0). Base‐promoted isomerisation of acetylene12provided allene11in a yield of 80%. 1‐(Pentafluorophenyl)‐3‐phenylprop‐2‐yn‐1‐ol2was prepared from phenylacetylene and pentafluorobenzaldehyde. The condensation of nicotinaldehyde with trimethylsilylacetylene gave the 3‐(trimethylsilyl)‐1‐(pyridine‐3‐yl)prop‐2‐yn‐1‐ol7. The removal of the silyl group of7to acetylene8was done in basic conditions. The Pd catalysed condensation of the acetylene8with iodobenzene gave 3‐phenyl‐1‐(pyridine‐3‐yl)prop‐2‐yn‐1‐ol9. The Pd catalysed condensation of8with 3‐bromopyridine gave the 1,3‐dipyridin‐3‐yl‐prop‐2‐yn‐1‐ol14.

ISSN:0009-4536    

DOI:10.1002/jccs.200700017

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

AbstractA gene of α‐L‐arabinofuranosidase (Abf) fromTrichoderma koningiiG‐39 was successfully expressed inPichia pastoris.The recombinant enzyme was purified to>90% homogeneity by a cation‐exchanged chromatography. The purified enzyme exhibits both α‐L‐arabinofuranosidase and β‐D‐xylosidase (Xyl) activities with p‐nitrophenyl‐α‐L‐arabionfuranoside (pNPAF) and 2,4‐dinitrophenyl‐β‐D‐xylopyanoside (2,4‐DNPX) as substrate, respectively. The stability and the catalytic feature of the bifunctional enzyme were characterized. The enzyme was stable for at least 2 h at pH values between 2 and 8.3 at room temperature when assayed for Abf and Xyl activities. Enzyme activity decreased dramatically when the pH exceeded 9.5 or dropped below 1.5. The enzyme lost 35% of Abf activity after incubation at 55 °C for 2 h, but retained 95% of Xyl activity, with 2,4‐DNXP as substrate, under the same conditions. Further investigation of the active site topology of both enzymatic functions was performed with the inhibition study of enzyme activities. The results revealed that methyl‐α‐L‐arabinofuranoside inhibition is noncompetitive towards 2,4‐DNPX as substrate but competitive towards pNPAF. Based on the thermal stability and the inhibition studies, we suggest that the enzymatic reactions of Abf and Xyl are performed at distinct catalytic sites. The recombinant enzyme possesses both the retaining transarabinofuranosyl and transxylopyranosyl activities, indicating both enzymatic reactions proceed

ISSN:0009-4536    

DOI:10.1002/jccs.200700018

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

AbstractA new compound: octaiodoanthraquinone (9,10) was synthesized from anthracene by a periodination process under the catalysis of mercury ions at 200˜250 °C. This new compound was also synthesized from anthraquinone (9,10) via a similar process, which verified the mercury‐catalyzed mechanism involved in the synthesis of octaiodoanthraquinone (9,10) from anthrac

ISSN:0009-4536    

DOI:10.1002/jccs.200700019

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY

摘要:

Abstract2,4‐Dinitrophenylhydrazones were converted to their parent carbonyl compounds using hexamethylenetetramine‐bromine complex supported onto wet alumina under classical heating and microwave irradiat

ISSN:0009-4536    

DOI:10.1002/jccs.200700020

出版商:WILEY‐VCH Verlag    

年代:2007

数据来源: WILEY