摘要:

Human glycodelin consists of 162 amino acid residues and twoN‐linked glycans at Asn28and Asn63. In this study, we synthesized it by a fully convergent strategy using native chemical ligation (NCL) in N to C direction. The four peptide segments corresponding to 1–31, 32–65, 66–105 and 106–162 sequences were synthesized by 9‐fluorenylmethoxycarbonyl based solid‐phase peptide synthesis. At the C‐terminus of the second segment,N‐ethyl‐S‐acetamidomethyl‐cysteine was attached as a post‐ligation thioesterification device. The N‐terminal two segments were condensed by the homocysteine‐mediated NCL at Leu‐Met site, and the product was methylated to convert homocysteine to methionine. After deprotection of acetamidomethyl group on theN‐ethylcysteine residue, the peptide was thioesterified byN‐alkylcysteine‐assisted method. The product was then ligated with the C‐terminal half, which was obtained by the NCL of third and fourth segments, to give the full‐length glycodelin. Copyright © 2013 Europea

ISSN:1075-2617    

DOI:10.1002/psc.2592

年代:2014

数据来源: WILEY

摘要:

No abstract is available for this article.

ISSN:1075-2617    

DOI:10.1002/psc.2688

年代:2014

数据来源: WILEY

ISSN:1075-2617    

DOI:10.1002/psc.2556

年代:2014

数据来源: WILEY