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1. |
Semisynthesis and optimization of G protein‐coupled receptor mimics |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 831-836
Sabine Abel,
Bernhard Geltinger,
Nadja Heinrich,
Dagmar Michl,
Annerose Klose,
Michael Beyermann,
Dirk Schwarzer,
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摘要:
We have recently developed a soluble mimic of the corticotropin‐releasing factor receptor type 1 (CRF1), a membrane‐spanning G protein‐coupled receptor, which allowed investigations on receptor–ligand interactions. The CRF1 mimic consists of the receptorN‐terminus and three synthetic extracellular loops (ECL1–3), which constitute the extracellular receptor domains (ECDs) of CRF1, coupled to a linear peptide template. Here, we report the synthesis of a modified CRF1 mimic, which is more similar to the native receptor possessing a cyclic template that displays the ECDs in a more physiological conformation compared with the initial linear design. In order to facilitate detailed biophysical investigations on CRF1 mimics, we have further established a cost‐efficient access to the CRF1 mimic, which is suitable for isotopic labeling for NMR spectroscopy. To this end, the loop‐mimicking cyclic peptide of the ECL2 of CRF1 was produced recombinantly and cyclized by expressed protein ligation. Cyclic ECL2 was obtained in milligram scale, and CRF1 mimics synthesized from this material displayed the same binding properties as synthetic CRF1 constructs. Copyright © 2014 European Peptide Society and John
ISSN:1075-2617
DOI:10.1002/psc.2680
年代:2014
数据来源: WILEY
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2. |
Peptides derived from the copper‐binding region of lysyl oxidase exhibit antiangiogeneic properties by inhibiting enzyme activity: anin vitrostudy |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 837-849
Arun Mohankumar,
Bhuvanasundar Renganathan,
Coral Karunakaran,
Subbulakshmi Chidambaram,
Sulochana Konerirajapuram Natarajan,
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摘要:
Despite the rigorous research on abnormal angiogenesis, there is a persistent need for the development of new and efficient therapies against angiogenesis‐related diseases. The role of Lysyl oxidase (LOX) in angiogenesis and cancer has been established in prior studies. Copper is known to induce the synthesis of LOX, and hence regulates its activity. Hypoxia‐induced metastasis is dependent on LOX expression and activity. It has been believed that the inhibition of LOX would be a therapeutic strategy to inhibit angiogenesis. To explore this, we designed peptides (M peptides) from the copper‐binding region of LOX and hypothesized them to modulate LOX. The peptides were characterized, and their copper‐binding ability was confirmed by mass spectrometry. The M peptides were found to reduce the levels of intracellular copper when the cells were co‐treated with copper. The peptides showed promising effect on aortic LOX, recombinant human LOX and LOX produced by human umbilical vein endothelial cells (HUVECs). The study also explores the effect of these peptides on copper and hypoxia‐stimulated angiogenic response in HUVECs. It was found that the M peptides inhibited copper/hypoxia‐induced LOX activity and inhibited stimulated HUVEC tube formation and migration. This clearly indicated the potential of M peptides in inhibiting angiogenesis, highlighting their role in the formulation of drugs for the same. Copyright © 2014 European Peptide Society and John W
ISSN:1075-2617
DOI:10.1002/psc.2675
年代:2014
数据来源: WILEY
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3. |
Antitumor efficacy, pharmacokinetic and biodistribution studies of the anticancer peptide CIGB‐552 in mouse models |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 850-859
Maribel G. Vallespí,
Gilmara Pimentel,
Ania Cabrales‐Rico,
Julio Garza,
Brizaida Oliva,
Osmani Mendoza,
Yolanda Gomez,
Tais Basaco,
Iraida Sánchez,
Carlos Calderón,
Juan C. Rodriguez,
Maria Rivera Markelova,
Iduna Fichtner,
Soledad Astrada,
Mariela Bollati‐Fogolín,
Hilda E. Garay,
Osvaldo Reyes,
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摘要:
Accumulation of the COMMD1 protein as a druggable pharmacology event to target cancer cells has not been evaluated so far in cancer animal models. We have previously demonstrated that a second‐generation peptide, with cell‐penetrating capacity, termed CIGB‐552, was able to induce apoptosis mediated by stabilization of COMMD1. Here, we explore the antitumor effect by subcutaneous administration of CIGB‐552 in a therapeutic schedule. Outstandingly, a significant delay of tumor growth was observed at 0.2 and 0.7 mg/kg (p < 0.01) or 1.4 mg/kg (p < 0.001) after CIGB‐552 administration in both syngeneic murine tumors and patient‐derived xenograft models. Furthermore, we evidenced that131I‐CIGB‐552 peptide was actually accumulated in the tumors after administration by subcutaneous route. A typical serine‐proteases degradation pattern for CIGB‐552 in BALB/c mice serum was identified. Further, biological characterization of the main metabolites of the peptide CIGB‐552 suggests that the cell‐penetrating capacity plays an important role in the cytotoxic activity. This report is the first in describing the antitumor effect induced by systemic administration of a peptide that targets COMMD1 for stabilization. Moreover, our data reinforce the perspectives of CIGB‐552 for cancer targeted therapy. Copyright © 2014 European Pe
ISSN:1075-2617
DOI:10.1002/psc.2676
年代:2014
数据来源: WILEY
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4. |
Synthesis and disaggregation of asparagine repeat‐containing peptides |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 860-867
Xiaomeng Lu,
Regina M. Murphy,
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摘要:
Of all amino acid repeats in eukaryotes, polyglutamine (polyQ) is the most frequent, followed by polyasparagine (polyN). Glutamine repeats are expanded in proteins associated with several neurodegenerative disorders. The expanded polyQ domain is known to induce aggregation, and it is hypothesized that aggregation is directly causative of pathology. Despite the widespread presence of asparagine repeats in invertebrate eukaryotes, polyN is curiously quite rare in vertebrates. Several investigators have characterized the conformational and aggregation properties of polyQ‐containing peptides and proteins, and to a lesser extent, peptides containing mixed glutamine and asparagine, but to our knowledge, there is no detailed characterization of polyN‐containing peptides. Such a comparison could elucidate reasons for the paucity of asparagine repeats in humans. In this study, we synthesized a peptide containing a 24‐asparagine repeat (N24). For aggregation studies, it is critical to start with monomeric unaggregated peptide. A protocol involving dissolution in mixed trifluoroacetic acid and hexafluoroisopropanol (TFA + HFIP) solvents is widely used for disaggregation of polyQ peptides. We used the same protocol for N24 but discovered that there was both oxidative damage and insufficient disaggregation. Oxidation of tryptophan, used as a flanking residue, was common. Moreover, we found evidence of Förster resonance energy transfer between Trp and its oxidation productN‐formylkynurenine, even in chemical denaturants. This suggested that N24 was insufficiently disaggregated, a conclusion that was further supported by gel electrophoresis analysis. Oxidation was reduced, but not eliminated, by addition of methionine to the buffer. Formic acid proved to be a better disaggregator and caused no oxidative damage. The glutamine repeat peptide Q24 also underwent some oxidation after extended incubation in TFA + HFIP, but there was no evidence of Förster resonance energy transfer, and samples appeared monomeric by gel electrophoresis. This result indicates that polyN‐containing peptides self‐associate more strongly than polyQ‐containing peptides. Circular dichroism spectra reveal a greater propensity forβ‐turn formation in polyN than polyQ, providing an explanation for the increased stability of polyN aggregates relative to polyQ. Copyright © 2014 European Peptide Socie
ISSN:1075-2617
DOI:10.1002/psc.2677
年代:2014
数据来源: WILEY
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5. |
Self‐assembled nanostructures of long‐acting GnRH analogs modified at position 7 |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 868-875
Ning Zhou,
Xing Gao,
Yujian Lv,
Junping Cheng,
Wenxia Zhou,
Keliang Liu,
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摘要:
It is well known that GnRH analogs can self‐assemble into amyloid fibrils and that the duration of action of GnRH analogs depends on the ability of the amyloid to slowly release active peptides. The aim of this study was to investigate the influence of the amino acid residues at position 7 of GnRH analogues on peptide self‐assembly. It was found that the dominant shape of the nanostructure can be changed when the structures of the residues at position 7 differ significantly from that of leucine in Degarelix. When the backbone length was extended (peptide 9), or the side chain of the residue at position 7 was replaced by an aromatic ring (peptide 6), or the rotation of the amide bond was restricted (peptide 8), the nanostructure changed from fibrils to vesicles. The results also indicate that the increasing hydrophilicity had little influence on the nanostructure morphology. In addition, a suitable release rate was found to play a more important role for the duration of the peptide action by maintaining the equilibrium between the drug concentration and the persistent release time, while the nanostructure shape was found to exert little influence on the duration of the peptide action. Copyright © 2014 European Peptide Society and John Wiley&Sons,
ISSN:1075-2617
DOI:10.1002/psc.2678
年代:2014
数据来源: WILEY
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6. |
Antimicrobial activities and membrane‐active mechanism of CPF‐C1 against multidrug‐resistant bacteria, a novel antimicrobial peptide derived from skin secretions of the tetraploid frogXenopus clivii |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 876-884
Junqiu Xie,
Yuanmei Gou,
Qian Zhao,
Kairong Wang,
Xiongli Yang,
Jiexi Yan,
Wei Zhang,
Bangzhi Zhang,
Chi Ma,
Rui Wang,
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摘要:
Hospital‐acquired infections caused by multidrug‐resistant bacteria pose significant challenges for treatment, which necessitate the development of new antibiotics. Antimicrobial peptides are considered potential alternatives to conventional antibiotics. The skin of Anurans (frogs and toads) amphibians is an extraordinarily rich source of antimicrobial peptides. CPF‐C1 is a typical cationic antimicrobial peptide that was originally isolated from the tetraploid frogXenopus clivii.Our results showed that CPF‐C1 has potent antimicrobial activity against both sensitive and multidrug‐resistant bacteria. It disrupted the outer and inner membranes of bacterial cells. CPF‐C1 induced both propidium iodide uptake into the bacterial cell and the leakage of calcein from large liposome vesicles, which suggests a mode of action that involves membrane disturbance. Scanning electron microscopy and transmission electron microscopy verified the morphologic changes of CPF‐C1‐treated bacterial cells and large liposome vesicles. The membrane‐dependent mode of action signifies that the CPF‐C1 peptide functions freely and without regard to conventional resistant mechanisms. Additionally, it is difficult for bacteria to develop resistance against CPF‐C1 under this action mode. Other studies indicated that CPF‐C1 had low cytotoxicity against mammalian cell. In conclusion, considering the increase in multidrug‐resistant bacterial infections, CPF‐C1 may offer a new strategy that can be considered a potential therapeutic agent for the treatment of diseases caused by multidrug‐resistant bacteria. Copyright © 2014 European Peptide So
ISSN:1075-2617
DOI:10.1002/psc.2679
年代:2014
数据来源: WILEY
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7. |
Interaction of a novel antimicrobial peptide isolated from the venom of solitary beeColletes daviesanuswith phospholipid vesicles andEscherichia colicells |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 885-895
Sabína Čujová,
Lucie Bednárová,
Jiřina Slaninová,
Jakub Straka,
Václav Čeřovský,
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摘要:
The peptide named codesane (COD), consisting of 18 amino acid residues and isolated from the venom of wild beeColletes daviesanus(Hymenoptera : Colletidae), falls into the category of cationicα‐helical amphipathic antimicrobial peptides. In our investigations, synthetic COD exhibited antimicrobial activity against Gram‐positive and Gram‐negative bacteria andCandida albicansbut also noticeable hemolytic activity. COD and its analogs (collectively referred to as CODs) were studied for the mechanism of their action. The interaction of CODs with liposomes led to significant leakage of calcein entrapped in bacterial membrane‐mimicking large unilamellar vesicles made preferentially from anionic phospholipids while no calcein leakage was observed from zwitterionic liposomes mimicking membranes of erythrocytes. The preference of CODs for anionic phospholipids was also established by the blue shift in the tryptophan emission spectra maxima when the interactions of tryptophan‐containing COD analogs with liposomes were examined. Those results were in agreement with the antimicrobial and hemolytic activities of CODs. Moreover, we found that the studied peptides permeated both the outer and inner cytoplasmic membranes ofEscherichia coli. This was determined by measuring changes in the fluorescence of probeN‐phenyl‐1‐naphthylamine and detecting cytoplasmicβ‐galactosidase released during the interaction of peptides withE. colicells. Transmission electron microscopy revealed that treatment ofE. coliwith one of the COD analogs caused leakage of bacterial content mainly from the septal areas of the cells. Copyright © 2014 European Peptide Soci
ISSN:1075-2617
DOI:10.1002/psc.2681
年代:2014
数据来源: WILEY
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8. |
Microwave‐assisted18O labeling of Fmoc‐protected amino acids |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 896-900
Maciej Modzel,
Halina Płóciennik,
Alicja Kluczyk,
Piotr Stefanowicz,
Zbigniew Szewczuk,
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摘要:
Recently, there has been an increased interest in isotopical labeling of peptides. Although there are several techniques allowing for a complete labeling of all carboxyl groups in peptides, regioselective labeling would be beneficial in many situations. Such labeling requires the use of18O‐labeled Fmoc amino acids. We have designed a method for such labeling that is an improvement on a technique proposed earlier. The new procedure is suitable for microscale synthesis and could be used in peptide and proteomics laboratories. Although for the majority of tested amino acids our method gives good labeling efficiency, it is time consuming. Therefore, we have decided to use microwave‐assisted procedure. This approach resulted in reduction of reaction time to 15 min and increased reaction efficiency. Copyright © 2014 European Peptide Society and John Wiley&Son
ISSN:1075-2617
DOI:10.1002/psc.2682
年代:2014
数据来源: WILEY
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9. |
The structure of cyclolinopeptide A in chloroform refined by RDC measurements |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page 901-907
Krzysztof Huben,
Michał Jewgiński,
Anna Pabis,
Piotr Paluch,
Burkhard Luy,
Stefan Jankowski,
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摘要:
Three‐dimensional structures of molecules traditionally assigned from nuclear Overhauser effects and vicinal coupling constants are recently complemented by measurements of residual dipolar couplings. Residual dipolar couplings measured in a stretched poly(dimethylsiloxane) gel were used to determine the structure of cyclolinopeptide A in chloroform solution at −50 °C. After structure refinement, conformational details of main cluster were discussed in relation to crystal and nuclear Overhauser effect derived structures. Copyright © 2014 European Peptide Society and John Wiley&So
ISSN:1075-2617
DOI:10.1002/psc.2683
年代:2014
数据来源: WILEY
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10. |
Issue information |
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Journal of Peptide Science,
Volume 20,
Issue 11,
2014,
Page -
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ISSN:1075-2617
DOI:10.1002/psc.2566
年代:2014
数据来源: WILEY
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