摘要:

AbstractBactenecin 5 (Bac 5) is an antibacterial 43mer peptide isolated from bovine neutrophils. It consists of an Arg‐richN‐terminal region and successive repeats of Arg‐Pro‐Pro‐Ile (or Phe). We synthesized Bac 51‐23and several related peptides to clarify the roles these regions play in antibacterial activity. An assay of antibacterial activity revealed that such activity requires the presence of Arg residues at or near theN‐terminus, as well as a chain length exceeding 15 residues. None of the peptides exhibited haemolytic activity. Polyproline II‐like CD curves were observed for most of the peptides. Measurements of the membrane perturbation and fusion indicated that the perturbation and fusogenic activities of the peptides were, generally, parallel to their antibacterial activities. Amino acid substitution in the repeating region had some effect on antibacterial activity. Copyright © 2001 European Peptide Society and John

ISSN:1075-2617    

DOI:10.1002/psc.317

出版商:John Wiley&Sons, Ltd.    

年代:2001

数据来源: WILEY

摘要:

AbstractFuc‐9 is the mature form of a vacuolar α‐L;‐fucosidase enzyme which seems to play an important role in plant growth regulation. Fuc‐9 is a 202‐residue protein containing five Cys residues located at positions 64, 109, 127, 162 and 169. In this study, the disulfide structure of Fuc‐9 was determined by MALDI‐TOF mass spectrometry (MS), with minimal clean‐up of the samples and at a nanomolar scale. Two strategies, based on a specific chemical cleavage (with 2‐nitro‐5‐thiocyanobenzoic acid and alkaline conditions) at the Cys residues and modification of Cys residues by acrylamide/deuterium labeled acrylamide alkylation, were used. Using these methods, the disulfide pairings Cys64‐Cys109 and Cys162‐Cys169 could be established. The advantages and limitations of our experimental approach are discussed. Copyright © 2001 European Peptide Socie

ISSN:1075-2617    

DOI:10.1002/psc.323

出版商:John Wiley&Sons, Ltd.    

年代:2001

数据来源: WILEY

摘要:

AbstractAn improved RP‐HPLC method was developed for the determination of the configuration and stereochemical purity of cysteine residues in peptides. The method consists of oxidation of cysteine and cystine residues to cysteic acid, followed by hydrolysis and pre‐column chiral derivatization with Val‐Marfey's reagent. Copyright © 2001 European Peptide Society and John Wiley&Son

ISSN:1075-2617    

DOI:10.1002/psc.325

出版商:John Wiley&Sons, Ltd.    

年代:2001

数据来源: WILEY

摘要:

AbstractChemical modifications of dual NK1/NK2ligand Cbz‐Gly‐Leu‐Trp‐OBzl(CF3)2(1) enabled us to create a high NK1selective ligand Cbz‐Pro‐Leu‐Trp‐OBzl(CF3)2(2). A determination of the conformational behavior of tripeptide2in solution is described. The 1D and 2D1H‐NMR techniques (COSY and ROESY) were used to assign resonances. Observed interproton distance restraints were considered to characterize conformational behavior. Spectral data indicate that tripeptide2presents a rigidified structure in DMSO stabilized by H‐bond in two γ‐turns. Agreement with experimental data was obtained by averaging the1H‐NMR parameters over several combinations of low‐energy conformations. Copyright © 2001 European Peptide Socie

ISSN:1075-2617    

DOI:10.1002/psc.326

出版商:John Wiley&Sons, Ltd.    

年代:2001

数据来源: WILEY

摘要:

AbstractOur interest is focused on the induction of allergic contact dermatitis (ACD) by the strong skin sensitizer, methyl octanesulfonate, which is a potent methyl transfer agent, especially to histidine and methionine residues. We are particularly interested to study the effect of methylation on the presentation and recognition of the ovalbumin (OVA) T‐cell epitope, OVA323‐339, by the T‐cell receptor (TCR). Here we report the synthesis of the modified monomerN‐α‐Fmoc‐N‐τ‐methyl‐

ISSN:1075-2617    

DOI:10.1002/psc.328

出版商:John Wiley&Sons, Ltd.    

年代:2001

数据来源: WILEY

摘要:

AbstractPolypeptide constructs containing up to four copies of the T cell epitope 306‐318 of influenza virus haemagglutinin have been synthesized on solid phase. Between the copies, a non‐natural PEG‐based spacer amino acid has been introduced. The oligomeric epitopes were analysed by RP‐HPLC and ES‐MS. The arrangement of the epitopes within the peptide constructs was either linear or comb‐like. The proliferative response in a T helper cell assay induced by these oligomerized epitopes has been tested, showing that the linearly arranged epitopes are more effective than the comb‐like oligomers. Copyright © 2001 European Peptide Society and John W

ISSN:1075-2617    

DOI:10.1002/psc.327

出版商:John Wiley&Sons, Ltd.    

年代:2001

数据来源: WILEY

ISSN:1075-2617    

DOI:10.1002/psc.347

出版商:John Wiley&Sons, Ltd.    

年代:2001

数据来源: WILEY