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1. |
Ultrafiltration, a useful method for isolation of intermediates in native chemical ligation exemplified with the total synthesis of Sortase AΔN59 |
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Journal of Peptide Science,
Volume 21,
Issue 4,
2015,
Page 257-264
Fang‐kun Deng,
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摘要:
In this paper, ultrafiltration was employed to facilitate the isolation of intermediates in native chemical ligation. Depending on the molecular weight cutoff of the membrane used, molecules with different sizes could be purified, separated, or concentrated by the ultrafiltration process. Total chemical synthesis of the polypeptide chain of the enzyme Sortase AΔN59was used as an example of the application of ultrafiltration in chemical protein synthesis. Sortase A is a ligase that catalyzes transpeptidation reactions between proteins that have C‐terminal LPXTG recognition sequence and Gly5‐ on the peptidoglycan of bacterial cell walls [3]. Ultrafiltration technique facilitated synthesis of Sortase AΔN59and was a promising tool in isolation of intermediates in native chemical ligation. Copyright © 2015 European Peptide Society and John Wiley&Son
ISSN:1075-2617
DOI:10.1002/psc.2745
年代:2015
数据来源: WILEY
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2. |
Novel p16 binding peptide development for p16‐overexpressing cancer cell detection using phage display |
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Journal of Peptide Science,
Volume 21,
Issue 4,
2015,
Page 265-273
Numfon Khemthongcharoen,
Athisake Ruangpracha,
Pongsak Sarapukdee,
Santi Rattanavarin,
Romuald Jolivot,
Ungkarn Jarujareet,
Kitiporn Plaimas,
Parvapan Bhattarakosol,
Suthiluk Patumraj,
Wibool Piyawattanametha,
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摘要:
Protein p16INK4a(p16) is a well‐known biomarker for diagnosis of human papillomavirus (HPV) related cancers. In this work, we identify novel p16 binding peptides by using phage display selection method. A random heptamer phage display library was screened on purified recombinant p16 protein‐coated plates to elute only the bound phages from p16 surfaces. Binding affinity of the bound phages was compared with each other by enzyme‐linked immunosorbent assay (ELISA), fluorescence imaging technique, and bioinformatic computations. Binding specificity and binding selectivity of the best candidate phage‐displayed p16 binding peptide were evaluated by peptide blocking experiment in competition with p16 monoclonal antibody and fluorescence imaging technique, respectively. Five candidate phage‐displayed peptides were isolated from the phage display selection method. All candidate p16 binding phages show better binding affinity than wild‐type phage in ELISA test, but only three of them can discriminate p16‐overexpressing cancer cell, CaSki, from normal uterine fibroblast cell, HUF, with relative fluorescence intensities from 2.6 to 4.2‐fold greater than those of wild‐type phage. Bioinformatic results indicate that peptide ‘Ser‐His‐Ser‐Leu‐Leu‐Ser‐Ser’ binds to p16 molecule with the best binding score and does not interfere with the common protein functions of p16. Peptide blocking experiment shows that the phage‐displayed peptide ‘Ser‐His‐Ser‐Leu‐Leu‐Ser‐Ser’ can conceal p16 from monoclonal antibody interaction. This phage clone also selectively interacts with the p16 positive cell lines, and thus, it can be applied for p16‐overexpressing cell detection. Copyright ©
ISSN:1075-2617
DOI:10.1002/psc.2726
年代:2015
数据来源: WILEY
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3. |
Activity of a novel‐designed antimicrobial peptide and its interaction with lipids |
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Journal of Peptide Science,
Volume 21,
Issue 4,
2015,
Page 274-282
Lanlan Yu,
Qiannan Fan,
Xiu Yue,
Yexuan Mao,
Lingbo Qu,
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摘要:
A new antimicrobial peptide l‐RW containing double amphipathic binding sequences was designed, and its biological activities were investigated in the present study. L‐RW showed antibacterial activity against several bacterial strains but low cytotoxicity to mammalian cells and low hemolytic activity to red blood cells, which makes it a potential and promising peptide for further development. Microscale thermophoresis (MST), a new technique, was applied to study the antimicrobial peptide–lipid interaction for the first time, which examined the binding affinities of this new antimicrobial peptide to various lipids, including different phospholipids, mixture lipids and bacterial lipid extracts. The results demonstrated that l‐RW bound preferentially to negatively charged lipids over neutral lipids, which was consistent with the biological activities, revealing the important role of electrostatic interaction in the binding process. L‐RW also showed higher binding affinity for lipid extract fromStaphyloccocus aureuscompared withPseudomonas aeruginosaandEscherichia coli, which were in good agreement with the higher antibacterial activity againstS. aureusthanP. aeruginosaandE. coli, suggesting that the binding affinity is capable to predict the antibacterial activity to some extent. Additionally, the binding of l‐RW to phospholipids was also performed in fetal bovine serum solution by MST, which revealed that the components in biological solution may have interference with the binding event. The results proved that MST is a useful and potent tool in antimicrobial peptide–lipid interaction investigation. Copyright © 2015 European Peptide Society and John W
ISSN:1075-2617
DOI:10.1002/psc.2728
年代:2015
数据来源: WILEY
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4. |
Conformational features and binding affinities to Cripto, ALK7 and ALK4 of Nodal synthetic fragments |
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Journal of Peptide Science,
Volume 21,
Issue 4,
2015,
Page 283-293
Luisa Calvanese,
Annamaria Sandomenico,
Andrea Caporale,
Annalia Focà,
Giuseppina Focà,
Gabriella D'Auria,
Lucia Falcigno,
Menotti Ruvo,
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摘要:
Nodal, a member of the TGF‐βsuperfamily, is a potent embryonic morphogen also implicated in tumor progression. As for other TGF‐βs, it triggers the signaling functions through the interaction with the extracellular domains of type I and type II serine/threonine kinase receptors and with the co‐receptor Cripto. Recently, we reported the molecular models of Nodal in complex with its type I receptors (ALK4 and ALK7) as well as with Cripto, as obtained by homology modeling and docking simulations. From such models, potential binding epitopes have been identified. To validate such hypotheses, a series of mutated Nodal fragments have been synthesized. These peptide analogs encompass residues 44–67 of the Nodal protein, corresponding to the pre‐helix loop and the H3 helix, and reproduce the wild‐type sequence or bear some modifications to evaluate the hot‐spot role of modified residues in the receptor binding.Here, we show the structural characterization in solution by CD and NMR of the Nodal peptides and the measurement of binding affinity toward Cripto by surface plasmon resonance. Data collected by both conformational analyses and binding measurements suggest a role for Y58 of Nodal in the recognition with Cripto and confirm that previously reported for E49 and E50.Surface plasmon resonance binding assays with recombinant proteins show that Nodal interactsin vitroalso with ALK7 and ALK4 and preliminary data, generated using the Nodal synthetic fragments, suggest that Y58 of Nodal may also be involved in the recognition with these protein partners. Copyright © 2015 European Peptide Society and John
ISSN:1075-2617
DOI:10.1002/psc.2733
年代:2015
数据来源: WILEY
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5. |
A novel chimeric peptide with antimicrobial activity |
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Journal of Peptide Science,
Volume 21,
Issue 4,
2015,
Page 294-301
Begum Alaybeyoglu,
Berna Sariyar Akbulut,
Elif Ozkirimli,
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摘要:
Beta‐lactamase‐mediated bacterial drug resistance exacerbates the prognosis of infectious diseases, which are sometimes treated with co‐administration of beta‐lactam type antibiotics and beta‐lactamase inhibitors. Antimicrobial peptides are promising broad‐spectrum alternatives to conventional antibiotics in this era of evolving bacterial resistance. Peptides based on the Ala46–Tyr51 beta‐hairpin loop of beta‐lactamase inhibitory protein (BLIP) have been previously shown to inhibit beta‐lactamase. Here, our goal was to modify this peptide for improved beta‐lactamase inhibition and cellular uptake. Motivated by the cell‐penetrating pVEC sequence, which includes a hydrophobic stretch at its N‐terminus, our approach involved the addition of LLIIL residues to the inhibitory peptide N‐terminus to facilitate uptake. Activity measurements of the peptide based on the 45–53 loop of BLIP for enhanced inhibition verified that the peptide was a competitive beta‐lactamase inhibitor with aKivalue of 58 μM. Incubation of beta‐lactam‐resistant cells with peptide decreased the number of viable cells, while it had no effect on beta‐lactamase‐free cells, indicating that this peptide had antimicrobial activity via beta‐lactamase inhibition. To elucidate the molecular mechanism by which this peptide moves across the membrane, steered molecular dynamics simulations were carried out. We propose that addition of hydrophobic residues to the N‐terminus of the peptide affords a promising strategy in the design of novel antimicrobial peptides not only against beta‐lactamase but also for other intracellular targets. Copyright © 2015
ISSN:1075-2617
DOI:10.1002/psc.2739
年代:2015
数据来源: WILEY
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6. |
Identification of FAK substrate peptides via colorimetric screening of a one‐bead one‐peptide combinatorial library |
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Journal of Peptide Science,
Volume 21,
Issue 4,
2015,
Page 302-311
Laurie A. Witucki,
Lauren Sanford Borowicz,
Anthony M. Pedley,
Jaime Curtis‐Fisk,
Elizabeth Girnys Kuszpit,
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摘要:
Focal adhesion kinase (FAK) is a protein tyrosine kinase that is associated with regulating cellular functions such as cell adhesion and migration and has emerged as an important target for cancer research. Short peptide substrates that are selectively and efficiently phosphorylated by FAK have not been previously identified and tested. Here we report the synthesis and screening of a one‐bead one‐peptide combinatorial library to identify novel substrates for FAK. Using a solid‐phase colorimetric antibody tagging detection platform, the peptide beads phosphorylated by FAK were sequenced via Edman degradation and then validated through radioisotope kinetic studies with [γ‐32P] ATP to derive Michaelis–Menton constants. The combination of results gathered from both colorimetric and radioisotope kinase assays led to the rational design of a second generation of FAK peptide substrates. Out of all the potential peptide substrates evaluated, the most active was GDYVEFKKK with aKM = 92 μM and aVmax = 1920 nmol/min/mg. Peptide substrates discovered within this study may be useful diagnostic tools for future kinase investigations and may lead to novel therapeutic agents. Copyright © 2015 European Peptide Society
ISSN:1075-2617
DOI:10.1002/psc.2751
年代:2015
数据来源: WILEY
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7. |
The interaction mechanism between lipopeptide (daptomycin) and polyamidoamine (PAMAM) dendrimers |
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Journal of Peptide Science,
Volume 21,
Issue 4,
2015,
Page 312-319
Boontarika Chanvorachote,
Jiang Qiu,
Walaisiri Muangsiri,
Ubonthip Nimmannit,
Lee E. Kirsch,
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摘要:
The interaction mechanism of lipopeptide antibiotic daptomycin and polyamidoamine (PAMAM) dendrimers was studied using fluorescence spectroscopy. The fluorescence changes observed are associated with daptomycin–dendrimer interactions. The binding isotherms were constructed by plotting the fluorescence difference at 460 nm from kynurenine (Kyn‐13) of daptomycin in the presence and absence of dendrimer. A one‐site and two‐site binding model were quantitatively generated to estimate binding capacity and affinity constants from the isotherms. The shape of the binding isotherm and the dependence of the estimated capacity constants on dendrimer sizes and solvent pH values provide meaningful insight into the mechanism of interactions. A one‐site binding model adequately describes the binding isotherm obtained under a variety of experimental conditions with dendrimers of various sizes in the optimal binding pH region 3.5 to 4.5. Comparing the pH‐dependent binding capacity with the ionization profiles of daptomycin and dendrimer, the ionized aspartic acid residue (Asp‐9) of daptomycin primarily interact with PAMAM cationic surface amine. Copyright © 2015 European Peptide Society and John
ISSN:1075-2617
DOI:10.1002/psc.2752
年代:2015
数据来源: WILEY
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8. |
Issue information |
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Journal of Peptide Science,
Volume 21,
Issue 4,
2015,
Page -
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ISSN:1075-2617
DOI:10.1002/psc.2693
年代:2015
数据来源: WILEY
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