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1. |
Cn‐AMP2 from green coconut water is an anionic anticancer peptide |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page 909-915
Saurabh Prabhu,
Sarah R. Dennison,
Manuela Mura,
Robert W. Lea,
Timothy J. Snape,
Frederick Harris,
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摘要:
Globally, death due to cancers is likely to rise to over 20 million by 2030, which has created an urgent need for novel approaches to anticancer therapies such as the development of host defence peptides.Cn‐AMP2 (TESYFVFSVGM), an anionic host defence peptide from green coconut water of the plantCocos nucifera, showed anti‐proliferative activity against the 1321N1 and U87MG human glioma cell lines with IC50values of 1.25 and 1.85 mM, respectively. The membrane interactive form of the peptide was found to be an extended conformation, which primarily included β‐type structures (levels > 45%) and random coil architecture (levels > 45%). On the basis of these and other data, it is suggested that the short anionic N‐terminal sequence (TES) ofCn‐AMP2 interacts with positively charged moieties in the cancer cell membrane. Concomitantly, the long hydrophobic C‐terminal sequence (YFVFSVGM) of the peptide penetrates the membrane core region, thereby driving the translocation ofCn‐AMP2 across the cancer cell membrane to attack intracellular targets and induce anti‐proliferative mechanisms. This work is the first to demonstrate that anionic host defence peptides have activity against human glioblastoma, which potentially provides an untapped source of lead compounds for development as novel agents in the treatment of these and other cancers. Copyright © 2014 European Peptide Socie
ISSN:1075-2617
DOI:10.1002/psc.2684
年代:2014
数据来源: WILEY
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2. |
Identification of linear B‐cell epitopes within Tarp ofChlamydia trachomatis |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page 916-922
Shanli Zhu,
Yan Feng,
Jun Chen,
Xiaoyun Lin,
Xiangyang Xue,
Shao Chen,
Xiaozhi Zhong,
WenShu Li,
Lifang Zhang,
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摘要:
Chlamydia trachomatisis one of the most prevalent sexually transmitted pathogens. There is currently no commercially available vaccine againstC.trachomatis. Chlamydial translocated actin‐recruiting phosphoprotein (Tarp) can induce cellular and humoral immune responses in murine models and has been regarded as a potential vaccine candidate. In this report, the amino acid sequence of Tarp was analyzed using computer‐assisted techniques to scan B‐cell epitopes, and six possible linear B‐cell epitopes peptides (aa80–95, aa107–123, aa152–170, aa171–186, aa239–253 and aa497–513) with high predicted antigenicity and high conservation were investigated. Sera from mice immunized with these potential immunodominant peptides was analyzed by ELISA, which showed that epitope 152–170 elicited serum immunoglobulin G (IgG) response and epitope 171–186 elicited both serum IgG and mucosal secretory immunoglobulin A response. The response of immune sera of epitope 171–186 to endogenous Tarp antigen obtained from the Hela229 cells infected withC.trachomatiswas confirmed by Western blot and indirect fluorescence assay. In addition, binding of the antibodies against epitope 171–186 to endogenous Tarp was further confirmed by competitive ELISA. Our results demonstrated that the putative epitope (aa171–186) was an immunodominant B‐cell epitope of Tarp. If proven protective and safe, this epitope, in combination with other well‐documented epitopes, might be included into a candidate epitope‐based vaccine againstC.trachomatis. Copyright © 2014 European Pept
ISSN:1075-2617
DOI:10.1002/psc.2689
年代:2014
数据来源: WILEY
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3. |
Synthesis of L‐Lys‐Aminoxy‐Goralatide |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page 923-927
Zhiliang Li,
Iryna Lebedyeva,
Deqian Zhao,
Lauren Myers,
Girinath G. Pillai,
Charles Dennis Hall,
Alan R. Katritzky,
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摘要:
Natural tetrapeptide Goralatide inhibits primitive hematopoietic cell proliferation but reported to be rather unstable in solution (half‐life 4.5 min). In this work, we report the synthesis of an aminoxy analog of Goralatide. Aminoxy moiety is expected to provide increased stability and bioavailability of the Goralatide analog. Copyright © 2014 European Peptide Society and John Wiley&Sons,
ISSN:1075-2617
DOI:10.1002/psc.2702
年代:2014
数据来源: WILEY
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4. |
Characterization of continuous B‐cell epitopes in the N‐terminus of glutamate decarboxylase67 using monoclonal antibodies |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page 928-934
Selin Agca,
Gunnar Houen,
Nicole Hartwig Trier,
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摘要:
Glutamate decarboxylase (GAD) is an autoantigen associated with the autoimmune disorders Type‐1 diabetes (T1D) and stiff‐person syndrome (SPS). The protein, being an essential enzyme involved in the production of the inhibitory neurotransmitter γ‐aminobutyric acid, exists in two isoforms, GAD67 and GAD65. Both isoforms may be targeted by autoantibodies in SPS and T1D patients, although SPS primarily is associated with the presence of GAD67 autoantibodies, whereas T1D mainly is associated with the presence of GAD65 autoantibodies.In this study, we describe antibody reactivity to overlapping GAD67 peptides covering the complete protein sequence by modified peptide enzyme‐linked immunosorbent assay in order to identify potential GAD67 epitopes using two monoclonal antibodies (mAbs).Both GAD67 mAbs showed reactivity to linear epitopes located at theN‐terminal end of GAD67. The epitopes of GAD mAb 1 and 2 were identified as the amino acid sequences NAGADPNTTN and TETDFSNLF, respectively, corresponding to amino acids 14–23 and 91–99. Fine mapping of the epitopes revealed that antibody reactivity was related to amino acid side‐chain functionality, rather than amino acid side‐chain specificity. Additionally, results suggested that non‐contact amino acids in the epitope structure were essential for antibody reactivity. The exact role of these amino acids remains to be determined, but they are thought to be involved in backbone hydrogen bonds or stabilization of the epitope structure.As only limited knowledge is available in relation to antigenic regions of GAD67, this study contributes to characterization of GAD67 epitopes and may be a first step in the development of peptide‐based therapeutics against SPS. Copyright © 2014 European Peptide Society a
ISSN:1075-2617
DOI:10.1002/psc.2703
年代:2014
数据来源: WILEY
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5. |
Structural insight into the transmembrane segments 3 and 4 of the hERG potassium channel |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page 935-944
Qingxin Li,
Ying Lei Wong,
Hui Qi Ng,
Shovanlal Gayen,
CongBao Kang,
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摘要:
The hERG (human ether‐a‐go‐go related gene) potassium channel is a voltage‐gated potassium channel containing an N‐terminal domain, a voltage‐sensor domain, a pore domain and a C‐terminal domain. The transmembrane segment 4 (S4) is important for sensing changes of membrane potentials through positively charge residues. A construct containing partial S2–S3 linker, S3, S4 and the S4–S5 linker of the hERG channel was purified into detergent micelles. This construct exhibits good quality NMR spectrum when it was purified in lyso‐myristoyl phosphatidylglycerol (LMPG) micelles. Structural study showed that S3 contains two short helices with a negatively charged surface. The S4 and S4–S5 linker adopt helical structures. The six positively charged residues in S4 localize at different sides, suggesting that they may have different functions in channel gating. Relaxation studies indicated that S3 is more flexible than S4. The boundaries of S3–S4 and S4–S4–S5 linker were identified. Our results provided structural information of the S3 and S4, which will be helpful to understand their roles in channel gating. Copyright © 2014 European Peptide Soci
ISSN:1075-2617
DOI:10.1002/psc.2704
年代:2014
数据来源: WILEY
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6. |
Design of novel analogues of short antimicrobial peptide anoplin with improved antimicrobial activity |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page 945-951
Yang Wang,
Jianbo Chen,
Xin Zheng,
Xiaoli Yang,
Panpan Ma,
Ying Cai,
Bangzhi Zhang,
Yuan Chen,
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摘要:
Currently, novel antibiotics are urgently required to combat the emergence of drug‐resistant bacteria. Antimicrobial peptides with membrane‐lytic mechanism of action have attracted considerable interest. Anoplin, a natural α‐helical amphiphilic antimicrobial peptide, is an ideal research template because of its short sequence. In this study, we designed and synthesized a group of analogues of anoplin. Among these analogues, anoplin‐4 composed ofd‐amino acids displayed the highest antimicrobial activity due to increased charge, hydrophobicity and amphiphilicity. Gratifyingly, anoplin‐4 showed low toxicity to host cells, indicating high bacterial selectivity. Furthermore, the mortality rate of mice infected withEscherichia coliwas significantly reduced by anoplin‐4 treatment relative to anoplin. In conclusion, anoplin‐4 is a novel anoplin analogue with high antimicrobial activity and enzymatic stability, which may represent a potent agent for the treatment of infection. Copyright © 2014 European Peptide Society and Joh
ISSN:1075-2617
DOI:10.1002/psc.2705
年代:2014
数据来源: WILEY
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7. |
Effect of head‐to‐tail cyclization on conformation of histatin‐5 |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page 952-957
Emilia Sikorska,
Elżbieta Kamysz,
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摘要:
Histatin‐5 (Hst‐5, DSHAKRHHGYKRKFHEKHHSHRGY) is a member of a histidine‐rich peptide family secreted by major salivary glands, exhibiting high fungicidal activity againstCandida albicans. In the present work, we demonstrate the 3D structure of the head‐to‐tail cyclic variant of Hst‐5 in TFE solution determined using NMR spectroscopy and molecular dynamics simulations. The cyclic histatin‐5 reveals a helix‐loop‐helix motif with α‐helices at positions Ala4‐His7and Lys11‐Ser20. Both helical segments are arranged relative to each other at an angle ofca. 142°. The head‐to‐tail cyclization increases amphipathicity of the peptide, this, however, does not affect its antimicrobial potency. Copyright © 2014 European Peptide So
ISSN:1075-2617
DOI:10.1002/psc.2707
年代:2014
数据来源: WILEY
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8. |
Efficient synthesis of polypeptide‐α‐thioester by the method combining polypeptide expression and chemical activation for the semi‐synthesis of interferon‐γ having oligosaccharides |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page 958-963
Yasuhiro Kajihara,
Yurie Kanemitsu,
Mika Nishihara,
Ryo Okamoto,
Masayuki Izumi,
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摘要:
In order to synthesize interferon‐γ glycoform having an oligosaccharide at the 97 position by a semi‐synthetic method, interferon‐γ‐polypeptide‐(1–94)‐α‐hydrazide was prepared by the specific Cys‐cyanylation of polypeptide‐(1–94)‐Cys‐His6expressed fromE. coliand subsequent hydrazinolysis in 22% yield (two steps). This polypeptide‐α‐hydrazide was then converted into corresponding polypeptide‐α‐thioester under NaNO2/acid conditions followed by thiolysis in 83% yield. Copyright © 2014 European P
ISSN:1075-2617
DOI:10.1002/psc.2709
年代:2014
数据来源: WILEY
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9. |
Issue information |
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Journal of Peptide Science,
Volume 20,
Issue 12,
2014,
Page -
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ISSN:1075-2617
DOI:10.1002/psc.2567
年代:2014
数据来源: WILEY
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