摘要:

AbstractA macrophage cell coating covering a cellulose acetate disk was an effective microenvironment for the production of peroxidase‐positive hematopoietic colonies. These developed after intraperitoneal injection of marrow cells with a linear cell relationship of dose to colonies formed. One colony formed for every 2,000 nucleated marrow cells injected. Observation of colony formation daily showed a steady increase in number and size until seven days after cell inoculation. X‐irradiation (400 rads) eliminated intrinsic colony formation in BALB/c mice. Irradiation of the donor of the ip marrow cells resulted in a Doof 95 rads. Treatment of the marrow donor with cytosine arabino‐side had a suppressive effect on colony formation as did treatment of the host animal after receipt of the ip marrow. These results indicate that the precursor of the granulocytic colonies seen in the macrophage layers are more similar to committed granulocytic precursors than to the pluripotential stem

ISSN:0361-8609    

DOI:10.1002/ajh.2830040202

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractUsing a fluorometric assay for the determination of oxidized pyridine nucleotides (NAD[P]+), total and cyanide‐resistant NADPH‐oxidative activities have been measured in subcellular fractions isolated from resting and phagocytosing human polymorphonuclears. Enzymatic activities responsible for the oxidation of the NADPH have been recovered in the heavy particles (15,000g/15 min), the low‐density particles (100,000g/30 min), and the cytosolic fraction. Stimulation of the cells with opsonized zymosan had a different effect on the NADPH‐oxidative activities of these subcellular fractions, which suggests the involvement of various types of enzymatic systems in the oxidation of NADPH. The cytosolic fraction interacted strongly with the enzymatic activities occurring in the sedimentable fractions and is therefore thought to play a central role in the regulation of the activation of the oxidative metabolism associated with phago

ISSN:0361-8609    

DOI:10.1002/ajh.2830040203

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractNucleated cells obtained from blood and/or bone marrow of patients with myelofibrosis with myeloid metaplasia (MMM) were cultured in diffusion chambers (DC) implanted into the peritoneal cavities of irradiated mice. A total of five blood studies and two bone marrow studies were performed using cells obtained from five patients. The DC were harvested at intervals from the host mice and the total and differential cellularity of DC contents were evaluated. The results obtained from MMM cultures were compared with those from similar cultures of blood cells and marrow cells of four and six normal individuals respectively. The proliferation and maturation of the granulocytic, erythrocytic, and megakaryocytic lines in MMM cultures occurred in an orderly fashion as they occur in vivo. The patterns of proliferation and maturation of the three cell lines in cultures after day 7 suggest that they primarily originate from progenitor cells. The numbers of granulocytes in the multiplicative pool, recognizable red cell precursors, and megakaryocytes recovered were significantly greater from the MMM cultures than those from the normal blood or marrow cultures. These results suggest that the blood and marrow cells of MMM patients have increased numbers of progenitors for granulocytes, erythrocytes, and megakaryocytes.

ISSN:0361-8609    

DOI:10.1002/ajh.2830040204

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractPeripheral blood from 16 patients with mycosis fungoides and two patients with Sézary syndrome was examined for T, B, and third‐population (K) cells, using a battery of surface markers. T lymphocytes as determined by spontaneous rosette formation with sheep erythrocytes and third‐population cells as determined by the Ripley rosette test were present in normal proportions. Surprisingly, B lymphocytes, as determined by surface immunoglobulin and receptors for mouse erythrocytes, were either lacking or were present in low proportions in some patients. Normal proportions were present in others. Repeat studies of two of three patients lacking B lymphocytes, following treatment, revealed normal or low proportions of B cells. Two patients with mycosis fungoides had increased proportions of “null” cells. Study of lymphoid cell subpopulations before and after leukopheresis in a single patient demonstrated a decrease in T cell proportions associated with a concomitant increase in the proportions of “null” cells following

ISSN:0361-8609    

DOI:10.1002/ajh.2830040205

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractA kindred with hereditary spherocytosis was found to have erythrocyte glutathione reductase deficiency. The glutathione reductase was evaluated enzymatically and immunologically. The enzyme activity could not be restored either by flavin adenine dinucleotide (FAD) in vitro, or by oral administration of FAD. Kinetic and electrophoretic studies, heat stability tests, and pH curves revealed no evidence of a variant enzyme. The results of electroimmunodiffusion and neutralization tests using an antiserum indicated that the deficiency is caused by a diminished production of enzyme protein. Hereditary spherocytosis and glutathione reductase deficiency traits segregated independently. The amount of reduced glutathione in the erythrocyte was normal, and the deficiency state without hereditary spherocytosis was asymptomatic. All deficient members in this kindred were heterozygous and the residual enzyme activity in the heterozygote might be sufficient to maintain the reduced glutathione level to prevent oxidative stress.

ISSN:0361-8609    

DOI:10.1002/ajh.2830040206

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractTwenty‐four cases of breast cancer and acute leukemia and three cases of breast cancer and chronic myelocytic leukemia are reported. An additional 54 cases of acute leukemia and 13 cases of chronic myelocytic leukemia associated with breast cancer from the literature are reviewed.The mean interval between the diagnosis of breast cancer and the occurrence of acute leukemia is 6.9 years. In eight patients, the two diseases occurred simultaneously or within one year of each other. Four patients had acute lymphoblastic leukemia; the remainder had acute myelocytic leukemia or one of its variants.Seven patients received no postmastectomy radiotherapy or chemotherapy and the occurrence of acute leukemia between 1 and 23 years later cannot, therefore, be attributed to the therapy given for the breast cancer. Forty‐one patients received postoperative radiotherapy to the mastectomy site. Combination chemotherapy was administered to eight patients, five of whom were also treated with radiotherapy.Acute leukemia is estimated to occur in patients treated for breast cancer with a sevenfold increased frequency over the expected number. This may be due to an increased risk of a second neoplasm in patients with a primary tumor; alternatively, the acute leukemia may be related to the radiotherapy and/or chemotherapy administered to treat the breast cancer. Late death from leukemia after chemotherapeutic or radiotherapeutic remission of metastatic breast cancer is preferable to morbidity and/or early death from inadequately treated breast can

ISSN:0361-8609    

DOI:10.1002/ajh.2830040207

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

ISSN:0361-8609    

DOI:10.1002/ajh.2830040208

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractTwenty‐three adult patients (median age 22 years) with haematological relapse of acute lymphoblastic leukaemia were given reinduction therapy with vincristine, adriamycin, prednisolone, and L‐asparaginase. Complete remission (CR) was achieved in 16 patients (69%), and only one patient was completely unresponsive to therapy. The duration of second remission was short except in three patients who had experienced long first remissions (>2.5 years). The median duration of survival from first relapse was seven mon

ISSN:0361-8609    

DOI:10.1002/ajh.2830040209

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractUltrastructural studies by scanning and transmission electron microscopy were carried out on one of four cases of platelet satellitism. Adherence to the neutrophils was mainly by focal contact between small surface projections of the platelets and neutrophils. Phagocytosis was not seen with the neutrophils but was evident in the monocytes. The degree of satellitism noted increased with the time the blood anticoagulated with EDTA was left at room temperature. Mixing experiments with a patient's plasma and control cells and the patient's cells and control plasma were inconclusive but suggested that the abnormality may reside in the plasma.

ISSN:0361-8609    

DOI:10.1002/ajh.2830040210

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractA 6‐year‐old child of northern European ancestry was found to have microcytic, hypochromic anemia with an elevated level of hemoglobin A2and an unbalanced pattern of globin chain synthesis characteristic of β‐thalassemia trait. Hematologic and globin synthesis studies of both parents yielded entirely normal results. Identification of the mother and father as the biological parents was established with a high order of reliability by determination of erythrocyte, serum, and HLA genetic markers. These findings suggest that the picture of β‐thalassemia observed in this child represents a new

ISSN:0361-8609    

DOI:10.1002/ajh.2830040211

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY