摘要:

AbstractA lupus‐type anticoagulant which causes strong inhibition of the partial thromboplastin time with kaolin (PTTK), the stypven time, and the thrombin generation tests has been investigated. All tests for platelet function were normal, as were all specific coagulation factor assays with the exception of a slightly reduced factor XI in this patient. A diethylaminoethyl‐cellulose‐immunoglobulin (DEAE‐cellulose‐IgG) fractionation of the patient's plasma produced two peaks containing inhibitory activity in the PTTK test. The first of these peaks had a cloudy appearance, suggesting the presence of immunoglobulin aggregates. Studies with IgG aggregates prepared from normal IgG and from the patient's IgG demonstrated that such aggregates were not the cause of inhibition. It was possible to neutralize the inhibitory activity of the purified IgG but not platelet‐poor plasma (PPP) with a rabbit anti‐IgG. The inhibition of the patient's PPP in the thrombin generation, the contact product, and the stypven time tests were corrected by the inclusion in the test system of platelets activated either by aggregation due to adenosine diphosphate (ADP) or formalin fixation and washing. These studies lend support to earlier findings that platelets interact at several sites in the coagul

ISSN:0361-8609    

DOI:10.1002/ajh.2830050202

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractTwenty‐three unselected adult acute leukemic patients were treated with an initial intravenous pulse of cytosine arabinoside (Ara‐C) 5 mg/kg. The mean mitotic index dropped from 7.7/1,000 to 2.8/1,000 at 4 hours after Ara‐C pulse and rebounded to 6.7/1,000 at 18 hours. Infusions of Ara‐C 7‐10 mg/kg were given for 9 or 12 hours, depending on changes in the mitotic index. The group of responders (M1or M2marrows) could be predicted by the initial blast count in the marrow and the change in the mitotic index 18 hours after the Ara‐C pulse. There were 9 (39%) complete and partial remissions. Six patients remained in remission more than 200 days. Since the changes in the mitotic indices induced by Ara‐C were indistinguishable from those of another more successful trial, we infer that factors other than modulation of the cell cycle are critical to the success of this mo

ISSN:0361-8609    

DOI:10.1002/ajh.2830050203

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractThe transfusion histories over a 33‐month period of 50 patients with sickle cell disease were reviewed to determine the frequency of alloimmunization to red cell antigens following transfusion in these patients. There were 30 females and 20 males, aged 19‐49 years. Eighteen (36%) were immunized of which thirteen were females. Five of the patients have formed only one antibody so far, while the other 13 have formed two or more. Thirty‐six antibodies were identified: 16 against various Rh antigens, 12 anti‐Lewis, 5 anti‐Kell and one each of anti‐Jka, ‐Fyaand ‐M. The immunized patients received, on the average, more transfusions although there was a considerable degree of overlap between the immunized and nonimmunized groups. An approach to the hemotherapy of patients with sickle cell disease (SC

ISSN:0361-8609    

DOI:10.1002/ajh.2830050204

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractA case of red cell adenosine deaminase (ADA) overproduction associated with hereditary hemolytic anemia is reported here. This appears to be the second report. Proband is a 38‐year‐old Japanese male who had hemoglobin, 15.8 g/100 ml; reticulocyte count, 4.5%; serum indirect bilirubin, 4.9 mg/100 ml;51Cr‐labeled red cell half‐life, 12 days; red cells showed moderate stomatocytosis. His red cell ADA activity showed 40‐fold increase while that of the mother showed 4‐fold increase. The mother was hematologically normal. The father had a normal enzyme activity. The proband and the mother showed slightly high serum uric acid levels. The proband's red cell showed: ATP, 628 nmoles/ml (normal, 1,010‐1,550); adenine nucleotide pool, 46% of the normal mean; 2,3‐diphosphoglycerate content, 3,782 nmoles/ml (normal 4,170‐5,300); increased oxygen affinity of hemoglobin, P50of intact crythrocytes being 21.8 mmHg (normal, 24.1‐26.1). Red cell glycolytic intermediates in the proband were low in general, and the rate of lactate production was low. Kinetic studies using crude hemolysate revealed a normal Km for adenosine, normal electrophoretic mobility but slightly abnormal pH curve and slightly low utilization of 2‐deoxyadenosine. The ADA activity of lymphocy

ISSN:0361-8609    

DOI:10.1002/ajh.2830050205

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractPlatelet function was investigated in four normal volunteers, one patient with a mild form of von Willebrand disease, and one with a thrombocytopathy, all taking propranolol. No effect on platelet function attributable to this drug could be demonstrated in any of these subjects. It is suggested that propranolol administered in conventional doses does not impair platelet hemostatic function.

ISSN:0361-8609    

DOI:10.1002/ajh.2830050206

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractIn the present experiments, we compare the behavior of erythropoietic precursors and granulocytic precurosors exposed to chloramphenicol. Subcutaneous injection of 20 mg of chloramphenicol sodium succinate on five successive days induced a reduction in the number of erythrocytic colony‐forming units (CFU‐E) and erythropoietic burst‐forming units (BFU‐E) in murine marrow and spleen, while no change in number of granulocytic precursors (CFU‐C) in murine hemopoietic organs was observed.In vitro studies demonstrated concentration‐dependent inhibition of chloramphenicol or chloramphenicol sodium succinate in the colony formation of all these precursors from murine and human bone marrow. However, erythropoietic burst formation of murine and human BFU‐E was completely suppressed by the addition of chloramphenicol or chloramphenicol sodium succinate to the culture medium at therapeutic concentrations (20‐40 μ/ml).From the present results, it can be concluded that the reduced number of BFU‐E in mice after injection of chloramphenicol sodium succinate resulted from the selective toxicity of chloramphenicol to BFU‐E at therape

ISSN:0361-8609    

DOI:10.1002/ajh.2830050207

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractFour new glucose 6‐phosphate dehydrogenase (G6PD) variants which had electrophoretically slow‐moving characteristics associated with congenital nonspherocytic hemolytic anemia were discovered in Japan. Gd(‐) Kurume was found in a 17‐year‐old male whose red cells contained only 0.8% of normal enzyme activity. Gd(‐) Kurume had a normal Km G6P, normal Km NADP, low Ki NADPH, normal utilization of 2‐deoxy‐G6P and deamino‐NADP, very low heat stability and a biphasic pH curve. The hemolytic anemia was mild. Gd(‐) Fukushima was characterized from a 33‐year‐old male and had 2.8% of normal enzyme activity, normal Km G6P, normal Km NADP, low Ki NADPH, normal utilization of 2‐dcoxy‐G6P, low utilization of deamino‐NADP, low heat stability and a normal pH curve. The hemolytic anemia was mild. Gd(‐) Yamaguchi was characterized from an 8‐year‐ol

ISSN:0361-8609    

DOI:10.1002/ajh.2830050208

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractThe present study was done in order to determine whether treatment with triodothyronine (T3) would improve the anemia of chronic renal insufficiency.Four patients with stable renal failure and anemia were treated with 75 μg/day of T3 for a period of four weeks. The patients were not in chronic dialysis, had normochromic normocytic anemia and hypocellular bone marrow, and showed no evidence of external blood loss during the study. They had received no iron, folic acid, or androgenic steroids for at lcast six months prior to the study.The administered dose of T3 was adequate to depress the serum levels of thyroid‐stimulating hormone (TSH); however, there was no detectable improvement in the levels of hemoglobin, hematocrit, bone marrow cellularity, serum and erythrocyte folate dcterminations, serum iron (Fc),59Fe halflife, plasma iron turnover rate, percentage Fe incorporated into red blood cells, or in the ratio of surface radioactive counting over bone marrow/liver and spleen.It is concluded that the experimental observations concerning direct bone marrow stimulation by T3 in anephric rats are probably not clinically applicable for the treatment of the anemia associated with urem

ISSN:0361-8609    

DOI:10.1002/ajh.2830050209

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractThe proliferative function of human and murine cadaveric bone marrow was studied using methylcellulose clonal cell culture assays and the murine spleen colony technique. The study revealed persistence of hemopoietic functions for as long as 19 postmortem hours in cadaveric marrows of some patients. Studies of murine cadaveric marrows corresponded with those of human marrows. These results strongly suggest that human pluripotent hemopoietic stem cells survive in cadaveric marrows.

ISSN:0361-8609    

DOI:10.1002/ajh.2830050210

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractA simple method for the permanent preservation of agar cultures is described. The technique involves fixation of agar cultures after incubation, drying, and subsequent remounting and staining on glass slides. The technique permits differential counting of colony type, approximate quantitation of cell numbers within individual colonies or clusters, and permanent preservation of cultures for purposes of interlaboratory standardization, and for routine reference in patient records.

ISSN:0361-8609    

DOI:10.1002/ajh.2830050211

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY