摘要:

Whole brains dissected from third-instar larvae ofDrosophila melanogasterincrease their cAMP content when incubated with octopamine, dopamine and serotonin, especially in the presence of theophylline, a phosphodiesterase inhibitor. The most pronounced rise in cAMP was produced by forskolin. When brains prelabelled with [32P]orthophosphate were subjected to these treatments, the increased labelling of 27.5-kDa protein(s) of pI 6.3-6.6 (substrate A) was observed in all cases, as revealed by two-dimensional gel electrophoresis and autoradiography. Labelling of substrate A was enhanced without any drug addition in brains of thedunceM11mutant strain, which has abnormally high basal cAMP levels. The labelling of substrate A was also promoted by the potassium channel blocker 4-aminopyridine. The data suggest that inDrosophilalarval brain the 27.5-kDa protein is a target of cAMP-triggered phosphorylation and may be related to potassium channel(s).

ISSN:0167-7063    

DOI:10.3109/01677068709167188

出版商:Taylor&Francis    

年代:1987

数据来源: Taylor

摘要:

Staggerer mutant mice, that lose cerebellar Purkinje cells and granule cells and inferior olive neurons, were evaluated in tests of spontaneous alternation and maze learning. It was found that, contrary to normal mice, the staggerer mutants did not alternate spontaneously and had deficits in the acquisition of maze learning. It is hypothesized that the olivocerebellar system has a role in visuo-spatial organization.

ISSN:0167-7063    

DOI:10.3109/01677068709167189

出版商:Taylor&Francis    

年代:1987

数据来源: Taylor

摘要:

We have cloned the cDNA encoding human liver glycogen phosphorylase (glycogenosis type VI) from a fetal brain cDNA library. Liver (L) and muscle (M) phosphorylase cDNA probes were used to determine the relative abundance of mRNA encoding the L- and M-isozymes of phosphorylase in human fetal and adult tissues. The transcript encoding the M-isozyme is 3.4 kb; the L-isozyme transcript is 3.3 kb. Transcriptional expression of the L-isozyme in human and primate tissues was found to differ from the isozyme's reported tissue specificity in non-primate mammals. Furthermore, using degenerate oligonucleotide probes to two different coding regions of M-phosphorylase, a novel 4.1-kb transcript was demonstrated to be present in human fetal and adult brain.

ISSN:0167-7063    

DOI:10.3109/01677068709167190

出版商:Taylor&Francis    

年代:1987

数据来源: Taylor

摘要:

Catecholamine- and serotonin-containing neurons were studied inpale, aDrosophilathird-chromosome recessive lethal mutant. Using histofluorescent and immunocytochemical techniques, we show that this mutation only alters catecholamine levels in the CNS. Both the presence of catecholamine-neurons and the expression of serotonin are not affected by the mutation. Furthermore, we show that normal characteristics of catecholamine neurons, such as the presence of the enzyme DOPA decarboxylase and the selective uptake properties are normal inpalemutants. We suggest thatpaleis either the tyrosine hydroxylase structural gene, or a gene controlling tyrosine hydroxylase activity inDrosophila. The similar genetic location of the putative tyrosine hydroxylase gene and the mutationpalesupports the former suggestion.

ISSN:0167-7063    

DOI:10.3109/01677068709167191

出版商:Taylor&Francis    

年代:1987

数据来源: Taylor