ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01513.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:Embryo sexing has been performed in both man and domestic species. Three approaches are described: H‐Y antigen, cytogenetic analysis and measurement of X‐linked enzyme activity. None of these requires the use of Y chromosome‐specific sequences. Sexing of semen by flow cytometry is discussed, this technique could eventually render embryo sexing obs

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01514.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:Y chromosome‐specific sequences derived to date from cattle, sheep and pigs are described. The methods used to isolate these sequences and possible future approaches are discussed. Three different techniques of sex determination are described: in situ hybridisation, Southern/Dot blotting and PCR based assays. All three methods utilise Y chromosome‐specific sequences. Necessary controls and precautions for PCR mediated assays are discus

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01515.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:A male specific bovine DNA fragment was used as probe to determine the sex of bovine interphase cells by in situ hybridization. This method also proved useful for determining the sex of bovine embryos.

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01516.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:A method of simultaneous amplification at three different loci (Y‐specific DNA, kappa‐casein gene, growth hormone gene) in cattle is presented. The use of this procedure for genetic typing on bovine crude DNA samples from less than ten diploid cells is shown. The application of this method for embryo genotyping and animal breeding is discus

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01517.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:Bovine embryos were used to evaluate the accuracy and the efficiency of sexing embryos by amplifying male specific DNA using the polymerase chain reaction. Blind tests with biopsies and biopsied embryos suggested that the method is accurate. However, often the amplification was unsuccessful and the assay therefore uninformative. We were able to amplify male specific DNA from degenerated embryos as well as from embryos stained with Hoechst 33342.

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01518.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:The establishment of some invasive techniques for bovine embryo sexing such as direct chromosomal evaluation, in situ hybridization using Y‐specific probe, and polymerase chain reaction for a Y‐specific DNA sequence is briefly reported. Advantages and disadvantages for their practical use are mentio

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01519.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:Embryo biopsy techniques with special reference to bovine morulae and blastocysts are reviewed. Techniques include methods involving holder pipettes and simplified methods more suitable for field use. The use of embryo biopsy for preimplantation diagnosis in combination with cattle embryo transfer is discussed.

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01520.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:Day 7 bovine embryos were microsurgically bisected and replaced into surrogate zonae pellucidae. They were fixed immediately after bisection and at various intervals of in vitro incubation at 35 °C in modified Dulbecco's medium. At the light microscopical level, the bisected embryos restored the prebisection morphology within 30 min. after splitting. The electron microscopy confirmed these findings, suggesting that day 7 bovine demi‐embryos for transfer purposes, should be cultured for 30 min before morphologically evaluated. Eleven pairs of bisected day 7 bovine embryos were transferred to 11 synchronized heifers. The recipient heifers were slaughtered at day 15, and the recovered embryos evaluated. Nine of the demi‐embryos developed to morphologically, normal spherical to elongated, emb

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01521.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contents:A method for in vitro culture of splitted frozen/thawed bovine embryos is described. These demi‐embryos are of a low viability, but they survive the treatments, which lead to the final cultivation. The culture system gave a development of 41%of demi‐embryos and 75 % of the whole embryos, and it is concluded, that this system is usable to culture demi‐em

ISSN:0936-6768    

DOI:10.1111/j.1439-0531.1991.tb01522.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY