摘要:

Suspensions of human oral epithetial cells were stained with antibodies to CD la and HLADR conjugated with fluorochromes and analysed by flow cytometry with the aim of purifying double‐labetled Langerhans cells, a population comprising approximatety2%of the cell total. Whole suspensions had high levets of autofluorescence and a wide range of forward and right angle scatter properties. The mean percentage of CDIalHLADR+ cells was 2.I%, though the double‐labetled cells did not form a discrete group and the percentages of positive cells using control antibodies were similar. Density gradient centrifugation prior to flow cytometry did not facilitate Langerhanr cell identification within the suspension. The results indicate flow cytometric analysis of minority cell populations (such as Langerhans cells) within oral epithetium is limited by the autofluorescence of physically heterogeneous keratinocytes, and emphasise the importance of controls in studies of oral epithetium which use this met

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00157.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

OBJECTIVE: To study the effect of cessation of tobacco use on the incidence of lichen planus, leukoplakia and other oral mucosal lesions.DESIGN: A 10–yr cohort study in a rural population of Ernakulam district, Kerala, India.MATERIAL AND METHODS: Some 12 212 tobacco users were interviewed and examined in a basetine survey and re‐examined annually for 10 years. At each examination they were exposed to health educational programs to encourage them to quit their tobacco use. The incidence rates were calculated using person‐years method among those who stopped their tobacco use and all others.RESULTS: A total of 77 681 person‐years of observation accrued among men and 32 544 among women. Among men 6.5% of these and among women 14.4% were in the stopped category. The incidence of oral lichen planus did not show any consistent association with cessation of tobacco habits (incidence ratio I.35) but for leukoplakia there was a substantial drop in the incidence after cessation (incidence ratio 0.3I). Several other tobacco‐associated oral mucosal lesions such as oral lichen planus‐like lesion, smoker's palate, preteukoplakia, central papillary atrophy of the tongue and leukoedema showed either zero, or very small incidence, after cessation. CONCLUSION: The reported association between tobacco use and lichen planus appears to be indirect but for all other lesions it is direct. The cessation of tobacco use led to a substantial fall in the incidence of leukoplakia and other lesions implying a reduced risk for oral cancer after cessation of

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00158.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY