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1. |
Expression of CDIa on monocytes cultured with supernatants from periodontally diseased gingival epithelial cells |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 247-252
T Winning,
E Gemmell,
B Polak,
NW Savage,
GJ Seymour,
LJ Walsh,
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摘要:
OBJECTIVE: Langerhans cells are believed to originate from the monocyte lineage and have been reported to increase in number with plaque accumulation and gingival inflammation. The aim of this study was to investigate the effects of local gingival epithelial factors on the induction of CDla, a Langerhans cell phenotype, on monocyte rich populations.MATERIALS AND METHODS: Peripheral blood monocyte rich populations from healthy subjects were cultured for 24 h with either healthy gingival or periodontally diseased gingival epithelial supernatants. Additionally, the monocyte rich populations were cultured with cytokines IL‐Iα, IL‐Iβ, IL‐6 and TNF‐α which are known to be produced by epithelial cells or co‐cultured with autologous epithelial cells. The percent CDIa positive cells was determined using FACS analysis.RESULTS: Healthy gingival supernatants did not induce CDIa expression in monocyte rich populations, however, a significant increase in per cent CDla+cells for monocyte rich populations cultured with five (P<0.01) of six periodontal gingival epithelial supernatants was found. IL‐lα or TNF‐α (10ng/well) resulted in a significant increase in the per cent CDla+cells (P<0.01). Depletion of CDla+Langerhans cells from healthy gingival epithelium did not enhance induction of CDIa expression in monocyte rich populations. Monocyte rich populations cultured together with non‐depleted epithelial cultures resulted in a decreased percent of CDla+cells.CONCLUSION: These findings indicated that epithelial factor/s associated with periodontally involved epithelia, may be involved in inducing a Langerhans cell phenotype in monocyte rich populations. The data also provide indirect evidence for a role of Langerhans cells in inhibiting induction of CDIa i
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00234.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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2. |
Rheumatoid factor from periodontitis patients cross‐reacts with epitopes on oral bacteria |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 253-262
J The,
JL Ebersole,
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摘要:
OBJECTIVES: The objective of this study was to determine the antigenic specificity of rheumatoid factor (RF) that had previously been reported in the serum of patients with periodontitis.DESIGN: IgM‐RF was isolated from the serum of five RF‐seropositive rheumatoid arthritis patients and 14 RF‐seropositive periodontitis patients and examined for specificity to human IgG and selected oral bacteria. METHODS: IgM‐RF was prepared by affinity chromatography on human IgG columns. Human IgG antibody toCapnocytophaga gingivalis, Fusobacterium nucleaturn, andActinobacillusactinomycetemcomitans was isolated by binding and elution of antibody from the bacteria, followed by purification using a rabbit anti‐lgG affinity column.MAIN OUTCOME MEASURE: Binding of the isolated IgM‐RF was determined using an enzyme‐linked immunosorbent assay (ELISA). The antigens used for detection of binding included isolated human IgG, human IgG antibody bound to the bacteria, and the bacteria alone. Inhibition of the IgM‐RF binding with IgG or Fcγ was used to assess the specificity of the reactivity with IgG and/or the bacteria.RESULTS: The results showed that the IgM‐RF reacted with polyclonal human IgG nonspecifically bound to microtiter plates. The reactivity of the IgM‐RF was increased when incubated with IgG that bound as antibody toC. gingivalis, F. nucleaturnorA. actinomyceternco‐mitans. However, the IgM‐RF did not bind with increased intensity to the specific IgG antibody preparations or to IgG preparations lacking antibody to these microorganisms. Additionally, the IgM‐RF preparations bound to surface components of bothC. gingivalisandF. nucleatum. Blocking studies showed that Fcγ but not IgG inhibited IgM‐RF binding to both micro‐organisms. CONCLUSIONS: These findings indicate that the RF detected in the serum of some periodontitis patients may be elicted by certain micro‐organisms in the subgingival plaque. Furthermore, C.gingivalisandF. nucleaturnappear to express surface antigen epitopes that are antigenically related to determinants on IgG and
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00235.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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3. |
Matrix metalloproteinases‐I, ‐3 and ‐8 and myeloperoxidase in saliva of patients with human immunodeficiency virus infection |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 263-271
L Mellanen,
T Ingman,
J Lähdevirta,
A Lauhio,
A Ainamo,
YT Konttinen,
A Sukura,
T Salo,
T Sorsa,
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摘要:
OBJECTIVE: Human immunodeficiency virus (HIV)‐sero‐positive patients have frequently severe gingival inflammation andlor attachment loss. In addition many infectious diseases affect their periodontium with varying clinical manifestations. Matrix metalloproteinases seem to play a key role in physiological periodontal remodelling and pathological tissue destruction, The aim of the present study was to characterize the presence, molecular forms, cellular sources, activities, and relative amounts of fibroblast‐type (matrix metalloproteinase [MMPJ‐I) and neutrophil (MMP‐8) collagenases, as well as their potential activator stromelysin‐I (MMP‐3) and myeloperoxidase in saliva of HIV‐seropositive patients at different phases of HIV‐infection. HIV‐seronegative, healthy, age‐matched patients served as controls.PATIENTS AND METHODS: Saliva samples were characterized by Western blotting using antibodies specific for MMP‐I, MMP‐3 and MMP‐8. Interstitial collagenase activities were measured using quantitative sodium dodecyl sulfate (SDS)‐polyacrylamide gel electrophoresis/laser densitometry assay. Myeloperoxidase was analysed using quantitative dot blotting. RESULTS: Clinical and microbiological evaluation of HIV‐seropositive patients' periodontium showed the presence of putative periodontopathogens ieActinobacillus actinomycetemcomitans (Ao), Porphyromonos gingivalis(Pg), Prevotellaintermedia (Pi), Peptostreptococcus micros(Psm) andCampylobacter rectus(Cr) in their periodontal pockets. The amount ofCandidaincreased with the severity of HIV‐infection. Clinical and microbiological findings of HIV‐seropositive patients suggested that they have a tendency to develop periodontal disease. Interstitial collagenase activities were found to be increased in saliva of different phases of HIV‐infected patients compared to the cont
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00236.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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4. |
Hydrolytic and depolymerising enzyme activity ofPrevotella intermediaandPrevotella nigrescens |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 272-278
S Stubbs,
MAO Lewis,
RJ Waddington,
G Embery,
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摘要:
OBJECTIVE:Prevotella intermediahas been reported to be associated with periodontal disease whilstP. nigrescenshas predominantly been isolated from more specific conditions and healthy sites. The aim of the present study was to compare the enzyme activity of these species.MATERIALS AND METHODS Nine strains of P.intermedioand 12 strains ofP. nigrescenswere studied. Lipolytic. saccharolytic, nucleolytic and proteolytic activity was determined by traditional microbiological and chromo‐genic substrate methods.RESULTS: All strains hydrolysed gelatine, casein. DNA and RNA. Lipase activity was produced by all strains except P. nigrescens ATCC 33563T. Lipolytic activity of P. nigrescens strains decreased as the environmental glucose concentration was increased. Only two strains, both P.intermedia, hydrolysed benzyl‐arg‐p‐nitroanilide. All strains hydrolysed alkaline pnitrophenolphosphate (except P.intermediaDAL 100). produced glycylprolyl dipeptidase activity and demonstrated elastase‐like activity. All but three strains (2 P.intermediaand I P. nigrescens) hydrolysed suc‐ala‐ala‐pro‐phe‐p‐nitroanilide. Overall, no qualitatively analysed enzyme activity was exclusive to all strains of either species. Quantitatively analysed activity exhibited a high degree of variability both within and between species.CONCLUSIONS: P.intermediaand P. nigrescens degrade natural and synthetic substrates, but intra‐ and interspec
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00237.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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5. |
Apoptosis‐associated proteins in oral hairy leukoplakia |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 279-284
E Chrysomali,
JS Greenspan,
N Dekker,
D Greenspan,
JA Regezi,
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摘要:
OBJECTIVE To test the hypothesis that the anti‐apop totic ability of Epstein‐Barr virus (EBV) may result in altered expression of apoptosis‐associated proteins in oral hairy leukoplakia (HL), we evaluated HL tissue and normal epithelium for these proteins by immunohistochemistry.MATERIALS AND METHODS Twenty formalin‐fixed, paraffin‐embedded specimens of HL lesions and six spedmens of normal control mucosa were selected from archived tissue specimens. Bcl‐2. Bcl‐x, Bax and p53 apoptoris‐associated proteins were evaluated in immu‐nohistochemically stained tissue sections according to staining intensity and pattern. The percentage of p53‐positive burl cells was estimated in sequential fields.RESULTS: Generalty, there were only slight differences in the expression of Bcl‐2 and Bcl‐x proteins in the epithelium of HL and control tissue. The staining for Bcl‐2 was weaker in keratinocytes than in putative melano‐cytes and Langerhans cells. Equivocal diffuse cytoplasmic staining of prickle cells was also noted. Keratinocytes throughout the epithelium stained positively for Bcl‐x protein, although upper layers were more weakly stained. The ‘balloon’ keratinocytes in HL were infrequently positive for Bcl‐x. Bax staining in HL differed from that in control tissue in being more heterogeneous. The staining reaction in HL was weak to negative in upper epithelial levels where ‘balloon’ keratinocytes were located. Weak to moderate nuclear p53 protein staining was detected in a mean of 25.3% of basal keratinocytes in all but one of the HL specimens; weak staining was seen in only two control specimens.CONCLUSIONS: We found only slight immunohistochemid evidence that expression of the apoptosis‐associated proteins is altered in HL. p53 appears to be over‐expressed in HL; we speculate that this may be related to upregulation or stabilization of w
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00238.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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6. |
Oral candidosis in long‐term hospital care: comparison of edentulous and dentate subjects |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 285-290
E Budtz‐Jørgensen,
P Mojon,
JM Banon‐Clément,
P Baehni,
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摘要:
OBJECTIVE: To obtain information on the oral health status of the elderly living in a medicalized, geriatric institution.DESIGN: A cross‐sectional clinical investigation with complementary microbiological studies.SUBJECTS AND METHODS: A cohort of 233 elderly in one long‐term care ward; collection of demographic data; clinical examination to determine dental and prosthetic status and health of the oral mucosae; swabs for detection of mucosal and denture colonization byCondido;paraffin stimulated saliva for detection of colonization by mutans streptococci and lactobacilli.MAIN OUTCOME MEASURES: Oral and denture hygiene; oral mucosal health; degree of colonization byCondida, mutans streptococci and lactobacilli.RESULTS: Mean age of the 233 patients was 85.6 ± 6.9 years; 61% were totally dependent, 62.7% were wearing one or two complete dentures; 19.7% had natural teeth and no denture and 17.6% neither teeth nor denture. Of those wearing dentures 72% had denture stomatitis. Of those with natural teeth 72% were affected by active caries. Yeast counts were significantly correlated with the intensity of the erythema of the palatal mucosa, plaque score of the natural teeth, denture plaque score, and salivary counts of mutans streptococci and lactobacilli.CONCLUSIONS: High oral yeast counts and frequent prevalence of oral candidosis in elderly subjects living in institutions are associated with poor oral hygiene and neglect of denture
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00239.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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7. |
Prevalence of oral leukoplakia in 1000 Berliners |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 291-294
PA Reichart,
H Kohn,
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摘要:
OBJECTIVE: The aim of this study was to determine the prevalence of oral leukoplakia and other oral white lesions in an urban population of non‐referred patients to a department of oral surgery in the city of Berlin.DESIGN: A total of 1000 patients over the age of 16 were evaluated for oral leukoplakia and other oral white lesions. Age, sex, and smoking as well as alcohol habits were recorded.RESULTS: Of 506 men (50.6%) and 494 women (49.4%), 0.9% showed oral leukoplakia. Men were more frequently affected (I.6%) than women (0.2%). Patients of older age groups were more frequently affected than younger patients. Other white oral lesions were recorded such as leukoedema (8.3%), smoker's palate (0.I%), frictional white lesions (2.6%) and lichen planus (0.6%) with equal distribution between men and women.CONCLUSION: The prevalence of oral leukoplakia in this limited group of urban patients was low, however comparable to that of other neighbouring west European countries. Association with tobacco and alcohol consumption was demonstrated as in most other studie
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00240.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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8. |
TCR Vβ gene expression in lesional T lymphocyte cell lines in oral lichen planus |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 295-298
XJ Zhou,
NW Savage,
PB Sugerman,
LJ Walsh,
MJ Aldred,
GJ Seymour,
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摘要:
OBJECTIVE: To study Vβ gene expression in oral lichen planus (OLP) lesional T lymphocyte cell lines.MATERIALS AND METHODS: Lesional T lymphocytes were isolated from eight OLP patients and cell lines established. The total RNA was extracted from these lymphocyte cell lines and reverse transcribed. cDNA was amplified by the polymerase chain reaction (PCR) using a panel of 26 Vβ‐specific oligonucleotide primers followed by qualitative analysis of the electrophoresed reaction products.RESULTS: Vβ 1, 2, 3, 5.1, 6.1–3, 7, 8, 9, 22. 23, and 24 were represented consistently in all of the OLP samples, Vβ11, 12, and 17 were consistently negative, while the other Vβ families (Vβ4, 5.2–3, 10, 13.1, 13.2, 14, 15, 16, 18, 19, 20, and 21) were variable. Vβ22 and 23 were the most strongly expressed in all patients.CONCLUSIONS A limited T cell receptor (TCR) gene usage indicates a degree of oligoclonality within these lesional T lymphocyte cell lines from OLP. This implies that OLP may be an antigen‐specific disease or linked to a limited number of
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00241.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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9. |
Pigmented lateral periodontal cyst and other pigmented odontogenic lesions |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 299-302
A. Buchner,
R David,
W Carpenter,
A Leider,
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摘要:
OBJECTIVE: To report a case of lateral periodontal cyst (LPC) with marked melanin pigmentation in a 38‐year‐old Black male and to discuss the phenomenon of melanin pigmentation in odontogenic cysts and tumors.RESULTS: Histologically, the epithelial lining of the LPC contained an abundant amount of melanin granules throughout the entire epithelium. Ultrastructurally, epithelial cells contained mature melanosomes (stage IV melanosomes). Melanophages containing aggregates of melanosomes were identified in the connective tissue cyst wall. Perusal of the literature revealed that melanin pigmentation in odontogenic lesions is uncommon. Melanin has been reported in calcifying odontogenic cyst (18 cases), odontogenic keratocyst (8 cases), adenomatoid odontogenic tumor (3 cases), ameloblastic fibroma (3 cases), odontoma (2 cases), and amelobastic fibro‐odon‐toma, odonto‐ameloblastoma, and odontogenic fibroma (I case each).CONCLUSIONS: Almost all pigmented odontogenic lesions occurred in Blacks and Asians; they are almost non‐existent in Whites. Thus, racial pigmentation probably plays an important role in s
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00242.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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10. |
Oral mucosal lesion associated with sickle cell disease |
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Oral Diseases,
Volume 2,
Issue 4,
1996,
Page 303-304
WW Evans,
MA Pogrel,
JA Regezi,
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摘要:
An oral lesion of sickle cell disease resembling a rapidly growing vascular lesion filled with sickled red cells is presented.
ISSN:1354-523X
DOI:10.1111/j.1601-0825.1996.tb00243.x
出版商:Blackwell Publishing Ltd
年代:1996
数据来源: WILEY
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