ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00159.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00160.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Porphyrornonasgingivalisdemonstrates a strong association with adult periodontitis although some individuals with the infection do not experience attachment loss. Therefore differences in the immune response to this organism may be of importance to the outcome of the disease.OBJECTIVE: The aim of this study was to determine whether P.gingivalispositive subjects with and without periodontal breakdown, reacted differently to P.gingivolisantigens as assessed by the pattern of serum antibody reactivity.MATERIALS AND METHODS: Two highly defined groups Of subjects were chosen for this study. Both demOnstrated pgingivalisin their plaque and both had responded to P.gingivolisas shown by the presence of serum antibodies*The two groups differed only in their apparent clinical susceptibility to periodontal breakd˜wn. Western blots of P.gingivolismembrane antigens were probed with sera from the two groups to determine their reactivity to specific antigens.RESULTS: Analysis Of the immunoblots showed that there were no differences in either the total numbers of bands, Or bands recognized by the majority Of subjects in the gingivitis and adult periodontitis groups. There were however, four bands recognized by the majority of the gingivitis group and not by the majority Of the adult periodontitis group, there being a significant difference (P = 0.03) in the recognition of the 91.4‐kDa antigen band*A further five antigens Of lower molecular weight were seen by the majority of the adult periodontitis group and not by the majorityofthe gingivitisgroup. When sera were tested against purified p*gingivalisLps, the results indicated that the five antigens seen by the majority Of the adult periodontitis group had molecular weights which were in the range exhibited by the Lps antigens. CONCLUSION: These results suggest that gingivitis and adult periodontitis subjects with P.gingivalisinfection, may recognize different P.gingivalisantige

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00161.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

OBJECTIVE: Trypsin‐like protease (TLPase) enzyme produced by Porphyromonasgingivalishas been implicated as a virulence factor in the pathogenesis of periodontal disease. The aims of this study were to investigate the relationship between cultivable P.gingivalis, TLPase enzyme activity (BANA hydrolysis) and serum antibody levels against cell sonicate and a purified TLPase antigen from P.gingivalisW50.MATERIALS AND METHODS: Sub‐gingival plaque samples were cultured for levels ofP. gingivalistogether with a chairside analysis of TLPase enzyme activity (Perioscan) from periodontitis and gingivitis sites of adult periodontitis patients. A TLPase from P.gingivaliswas purified by gel filtration and ion exchange chromatogra‐phy from the vesicle fraction for use as a test antigen. RESULTS: Elevated levels of P.gingivaliswere found at periodontitis sites, however, there was no correlation with sub‐gingival plaque TLPase enzyme activity. Adult periodontitis patients had higher levels of IgG and IgA against cell sonicate and TLPase antigens than did controls. Those patients who were P.gingivalisculture‐positive demonstrated an elevated immune response against both cell sonicate and TLPase when compared to P.gingivalisculture‐negative patients. Treatment resulted in an improvement of clinical indices and no cultivable P.gingivaliscould be recovered from the treated sites and there was a concomitant decrease in IgG levels against the TLPase. There was no significant difference in BANA hydrolysis at gingivitis sites or periodontitis sites after treatment.CONCLUSIONS Further longitudinal studies are suggested to investigate the role of the TLPase in the response to treatment of chronic adult periodontit

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00162.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

A 59‐year‐old Caucasian male patient with AIDS is described who presented with an oral lesion of cryptococcosis. The gingival ulceration was the only detectable lesion of cryptococcosis. Diagnosis was established by his‐topathologic findings from biopsy and detection of serum cryptococcal antigen. The patient was treated with amphotericin B and flucytosine. After four weeks of therapy cryptococcal antigen turned negative. The oral ulceration diminished in size, but an inflammatory tissue reaction pers

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00163.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

OBJECTIVE: Topically applied aluminum (AI) reduced fissure caries in rats with indigenous microflora, but neither therapeutic activity nor concentration effects have been studied in animals infected with caries‐inducing bacteria. This study investigated the effects of solutions containing various Al concentrations on formation of smooth surface and sulcal caries in cariogenically challenged rats infected with Streptococcus sobrinus.METHODS: Weanling rats, inoculated withS. sobrinus, were randomly assigned to six groups which were topically treated twice daily with water (treatment control), a NaF solution of 100 parts/106F (positive control), or AIK(SO4)2solutions containing 100, 1000, 2000, or 4000 parts/106Al. Rats were fed a high‐sucrose diet and distilied waterad libitumfor 10 weeks, then the molars were scored for enamel and dentin caries.RESULTS: Relative to the water control, NaF and all Al solutions significantly reduced prevalence of both smooth‐surface and fissure caries. Protection by Al solutions was greater on smooth surfaces than sulcal areas. Al treatments reduced smooth surface scores by 49–7I% and sulcal scores by 27–53%. For all comparisons the protective effect of the 100 parts/106Al solution was less than solutions containing 1000 parts/106Al or more. NaF and all Al solutions also significantly reduced the extent of lesion penetration into dentin.CONCLUSIONS: Topically applied AI reduced the formation and progression of both smooth surface and Sul. cal caries and showed evidence of a dose response in a rat model infected with S.

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00164.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Saliva is frequently used as a diagnostic fluid and several collection devices have been developed.OBJECTIVE: The aim of the present study was to investigate the validity and reliability of two types of Salivette®collection kits (non‐covered cotton roll and polypropylene covered polyether roll) relative to conventional collection of saliva using paraffin wax chewing stimulation.MATERIALS AND METHODS: Whole saliva samples were collected from 16 healthy volunteers. Following a cross‐over design saliva was collected in a standardized way. The flow rate was determined and saliva samples were analyzed for pH, buffer capacity, electrolytes and protein/glycoprotein content.RESULTS: We find that Salivette® methods do not allow evaluation of flow rate. pH was unaffected but buffer capacity was lower in Salivette® collected than in paraffin wax‐stimulated saliva. The non‐covered cotton rolls reduced the content of Na+K+CI‐as well as glycoprotein markers (hexosamines, fucose, sialic acid), lysozyme, lactoferrin, salivary‐ and myeloperoxidase but increased the concentrations of Ca2+PO3–4and SCN‐. Polypropylene covered polyether rolls affected saliva composition less than the non‐covered cotton rolls. Thus, SCN‐ and slgA concentrations were higher and lysozyme activity lower in the former (covered roll) saliva than in paraffin wax saliva. The reliability of the Salivette® kits was good.CONCLUSION: We conclude that the Salivette® method generates data significantly different from conventional paraffin wax‐stimulated saliva such as buffer capacity and several electrolytes and organic components. Care should be taken in interpreting the results when

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00165.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

OBJECTIVE: TGF‐β I‐coated β‐TCP pellets were grafted in experimental defects of rat calvariae to study the effects on new bone formation.MATERIALS AND METHODS: The grafted sites were evaluated by light microscopy using hematoxylin‐eosin (H‐E) staining for histology and detection of alkaline phosphatase (ALPase) and tartrate‐resistant acid phos‐phatase (TRACPase) activities to demonstrate osteo‐blastic and osteoclastic cells. Confocal laser scanning microscopy (CLSM) was performed for morphometry of newly formed bone.RESULTS: The sections showed more new bone formation in sites grafted with TGF‐β I ‐coated β‐TCP pellets (experimental sites) than those with β‐TCPpellets only (control sites). TRACPase‐positive and ALPase‐positive cells at experimental sites were more frequent than at control sites. The bone formation rate calculated by computerized CLSM pixel image analysis showed more new bone formation at the experimental sites than at control sites (3.4 ± 0.8% vs 9.3 ± 1.7% on week 2 and 11.8 ± 2.1% vs 39.8 ± 10.9% on week 4).CONCLUSION: TGF‐P β I ‐coated β‐TCP pellets promote new bone formation and may be

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00166.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00167.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:1354-523X    

DOI:10.1111/j.1601-0825.1995.tb00168.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY