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21. |
A Quantitative Assay for a Clara Cell-Specific Protein and Its Application in the Study of Development of Pulmonary Airways in the Rat |
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Pediatric Research,
Volume 20,
Issue 8,
1986,
Page 802-805
GURMUKH SINGH,
SIKANDAR KATYAL,
MARI-LOU WONG-CHONG,
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摘要:
Rat lung lavage contains a 10 kDa protein that has been shown by immunocytochemistry to be specific for Clara cells. An inhibition enzyme-linked immunosorbent assay was established for this protein using rabbit antibody to the 10 kDa Clara cell protein. The assay has a sensitivity of about 3.0 ng/ml and a working range of about 5 to 50 ng/ml. Quantitation of the 10 kDa protein in amniotic fluid revealed an increase of about 4-fold at day 20 of gestation. The 10 kDa protein content of lung homogenate increased steadily from day 18 of gestation to 1 wk after birth, after which a decline was observed. Nearly 60-fold increase in the concentration of the 10 kDa Clara cell protein in lungs was noted from day 18 of gestation to birth and a further about 7-fold increase was noted from the day of birth to 1 wk of age. A progressive increase in the 10 kDa protein, with increasing age, was also noted on immunoblot analysis of lung homogenates. As judged from the immunoblots of lung homogenates, stained with rabbit antirat 10 kDa protein antiserum, the content of an antigenically similar 200 kDa Clara cell protein was negligible. The quantitative results for 10 kDa Clara cell protein parallel the results of immunocytochemistry and quantitation of the volume density of Clara cell granules indicating that quantitation for the 10 kDa protein could be used to monitor the development of Clara cells and that of the pulmonary airways.
ISSN:0031-3998
出版商:OVID
年代:1986
数据来源: OVID
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22. |
Carnitine during Prolonged Breast Feeding |
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Pediatric Research,
Volume 20,
Issue 8,
1986,
Page 806-809
LIISA ROVAMO,
LEENA SALMENPERÄ,
PIRKKO ARJOMAA,
KARI RAIVIO,
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PDF (438KB)
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摘要:
To assess carnitine levels during prolonged sole breast feeding we measured serum and breast milk carnitine concentrations in 37 lactating mothers and their healthy term infants from birth to the age of 1 yr. The number of solely breast-fed infants decreased to 31 at 2 months of age, to 28 at 6 months, and to seven at 9 months, because formula and/or solid food was added when there was not enough breast milk. In mothers the mean serum carnitine increased from 35 to 50 μmol/liter during the first 2 months after delivery and remained unchanged thereafter. Irrespective of the type of feeding, the mean serum carnitine in infants increased from 29 to 59 μmol/liter during the first 2 months, remained unchanged during 2-9 months, and decreased to the mean level of mothers thereafter. The mean carnitine concentration of breast milk was high (106 μmol/liter) immediately after delivery. During the first 2 months the mean carnitine concentration of milk decreased to the mean serum level of mothers and remained unchanged thereafter. The carnitine concentrations of serum and breast milk did not correlate, however. The mean daily carnitine intake of the breast-fed infants was 5.7 μmol/kg at 4 months of age, 4.7 μmol/kg at 6 months, and 6.0 μmol/kg at 9 months whereas the mean daily carnitine intake of the infants receiving formula was 28.9 μmol/kg at 1 month of age and 30.7 μmol/kg at 2 months. The serum concentration of carnitine in our infants did not correlate with carnitine intake. Our results indicate that serum carnitine concentrations are maintained during prolonged sole breast feeding.
ISSN:0031-3998
出版商:OVID
年代:1986
数据来源: OVID
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