摘要:

AbstractA recent study (see preceding article) was conducted to evaluate bacterial mutagenicity of extracted particulates collected from air in Houston, Texas. Initially the solvent system used to extract these particulates was the 1:1:1 v/v mixture of benzene, methanol, and dichloromethane used by Pitts et al [1] in a similar study in California. Because of recent concern about the genetic toxicity of benzene, efforts are being made, where possible, to substitute other solvents, and a suggestion was made to try cyclohexane.The recent National Institute for Occupational Safety and Health (NIOSH) documents on exposure to coal tar [2] and to asphalt fumes [3]have recommended use of cyclohexane to extract air samples from occupationally exposed air filters. In addition, recent publications [4–6] have used cyclohexane to extract polyaromatic hydrocarbons (PAH) and aliphatic hydrocarbons from particulate air samples. A study done by Stanley et al [7]found cyclohexane almost 100% efficient at extracting benzo(a)pyrene. The present study was designed to examine the difference in efficiency of extraction between cyclohexane and a 1:1:1 mixture of benzene, methanol, and dichloromethane, using the Ames assay as a test for mutagenicit

ISSN:0192-2521    

DOI:10.1002/em.2860020112

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1980

数据来源: WILEY

摘要:

AbstractAlthough bleomycin (BLM) induces structural chromosome damage, eg, in human lymphocytes, no, or at best a weak, mutagenicity of this substance has been observed after the application of two well‐established mutagenicity screening procedures, the Ames test and the sister‐chromatid exchange (SCE) test. After feeding BLM to Drosophila melanogastermaleswe, too, observed only a weak mutagenicity as measured by the frequency of recessive sex‐linked lethal mutations. These results are based on the analysis of postmeiotic germ cell stages (spermatozoa, spermatids). No autosomal translocations were found in the same experiments. BLM was also fed to Drosophilafemalesunder conditions similar or identical to those of the experiments with males. We observed a considerable sensitivity of Drosophila oocytes to the induction by BLM of recessive sex‐linked lethal mutations and X‐chromosomal aneuploidy (nondisjunction and chromosome loss). Our oocyte results demonstrate that the extensively used antitumor agent bleomycin has to be considered as

ISSN:0192-2521    

DOI:10.1002/em.2860020113

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1980

数据来源: WILEY

ISSN:0192-2521    

DOI:10.1002/em.2860020114

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1980

数据来源: WILEY

ISSN:0192-2521    

DOI:10.1002/em.2860020101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1980

数据来源: WILEY