ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01691.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

The use of a lymphocyte transformation test (LIT] to confirm allergic contact dermatitis from thiurms has been investigated. The responses of peripheral blood mononuclear cell? (PBMC) from thiuram‐sensitive; and non‐sensitive individuals following culture with dimethylerbamoyl‐protein (human serum albumin; USA) and dimethylthiocarbamoly‐HSA conjugates has been compared. Only PBMC From those patients who were patch‐test‐positive with thiuram‐mix and sensitized to tetramethylthiuram monosulphide (TMTM) or TMTM and tetramethylthiuram disulphide (TMTD) exhibited significant proliferative responses to these conjugates. Thiuraiu‐patch‐test.‐negative patients and control donors with no history fo allergic contact dermatitis failed to mount a significant response to any concentration of either conjugate. Two of the thiuram sensitive patients were also nickel‐patch‐test‐positive. and PBMC isolated from these donors, but not from nickel‐patch‐test‐negative patients, proved positive in a nickel LTT. The data reveal that relevant hapten‐protein conjugates are capable of provoking spicifie human lymphocyte proliferative responses in vitro, and that, using this technique, the LTT can, in principle, be used for the investigation and/or diagnosis of skin sensitizatio

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01692.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

The laser‐Doppler blood flow and erythema index were assessed in 16 patch test reactuibs caysed by irritants (1% aq. Sodium lauryl sulphate and 1%aq. Benzalkonium chloride) and in 13 varied allergic reactions, at 2 or 4 days. Both irritant and allergic responses produced satistically significant increases in laser‐Doppler flow index and erythema index compared to control sites (p<0.05 or less, using the Wilcoxon rank‐sum test). A disproportionately greater increase in erythema index than in laser‐Doppler flow was seen in mild irritant reactions, compared to allergic, though the two could no be reliably distinguished by these tests. 2 non‐allergic nickel sulphate sites produced an increase in both blood flow and erythema without any clinical change. Petrolatum alone produced no significant change in either measurement. The laser‐Doppler flow showed an overall correlation with the erythema index (product moment method:r=0.55.p<0.001), but there was little correlation between these indices and patch test reactivity as judged clinically by conventional scoring. Generally, allergic and irritant patch test reactions could not be differentiated on the basis of laser‐Doppler flow index for mild irritant responses warrants further study in other ir

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01693.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

We patch tested 141 patients with 0.05% aq thimerosal and 222 patients with 0.05% aq, mercuric chloride, including 63 children. The frequency of positive patch test reactions to thimerosal was 16.3%. There was a marked preponderance in the young age groups after vaccination, while none of 3h infants (aged 3–48 months) reacted lo thimerosal positive reactions to mercuric chloride were found in 23 (10.4%) of 223 patients. We also sensitized guinea pigs with diphtheria‐pertussis‐tetanus (DPT) vaccine containing 0.01% thimerosal and succeeded in inducing hypersensitivity to thimerosal. From patch testing in humans and animal experiments, it is suggested that 0.01% thimerosal in vaccines can sensitize children, and that hypersensitivity to thimerosal is due to the throsaticylic part of the molecule and correlates with photosensitivity to piro

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01694.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Little is yet known about the duration of contact sensitivity, but frequent exposure of a target lo allergen seems to reduce skin reactivity The aim of this study was to study the persistence of a specific contact sensitivity in 66 patients with alopecia areata, previously sensitized in DNCB (31 patients) and SADBF, (35 patients) between 1978–1985. Patch tests were performed with 0.020 ml of different concentrations of DNCB or SADBE in acetone (0.05%, 0.10%. 0.20% 1%). The results were read in a standardized manner. Of 66 patients, 47 (71%) had positive reactions and 19 (29%) negative. 8 of the 19 negative patients had been treated with DNCB, 11 with SADBE, Approximately 1/3 of the patients previously sensitized had lost their original sensitivity, und this did not seem to be time‐dependent. This phenomenon seemed to be clinically correlated because the majority of the patients were from the “low responders” group. We think that acquired unresponsiveness to topical antigen in man to a possible phenomenon, but that it occurs more rarely than in mice and guin

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01695.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

17 patients allergic to thimerosal. with no previous history of photosensitivity or piroxicam ingestion. 2 patients with piroxicam‐induced photosensitivity, and 10 controls were patch or photopatch tested, with 1 or more of the following thimerosal, thiosalicylic acid, irradiated and non‐irradiated solutions of piroxicam alone, L‐cysteine alone and piroxicam plus l‐cysteine, and piroxicam in petrolatum, The results of the tests supported the hypothesis that there are cross reactions between thiosalicylic acid and piroxicam, a photosensitizer. The mechanism of the cross‐reactions may involve photoproduts of piroxicm and L‐cysteine, as patients allergic to thiosalicylic aid, who have positive photopatch tests to piroxicam, also had positive patch tests to the irradiated solution of piroxicam plus L

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01696.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contact dermatitis due to garlic is usually due to handling of garlic for cooking. Among the Chinese, garlic is also used its a form of topical medicament S patients developed contact dermatitis lifter rubbing the cut end of a fresh garlic bulb onto the skin to treat fungal and other inflictions at the groin, neck, lower limb, hand or face. The distribution and morphology of the lesions were different from the classical form as described in the literature. Repeated open application tests with fresh garlic were all positive and patch tests with garlic extract were all negative. 5 controls tested by repeated open application with fresh garlic juice were also positive and patch tests in 10 controls with garlic extract were also negative. The results confirmed that the contract dermatitis was due to irritation. The patients were treated successfully with topical fluorinated steroid. For prevention, the practice of direct application of fresh garlic onto the skin for treating infections should be discouraged.

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01697.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

An outbreak of acute pruritic rash occurred in Mutch 1990 among 141 residents of a high‐rise public housing estate in Bukit Panjang, Singapore. The typical rash consisted of urticarial lesions distributed over the limbs and trunk. The outbreak was associated with a transient increase in tussock moths in the residential estate following an unusual, short dry spell. The aetiology was established when patch tests with crude moth material produced similar eruptions in 5 out of 7 adult volunteers between 40 min and 12 h. Pharmacological experiments with an aqueous extract of moth hairs in isolated guinea pig ileum elicited a response similar to that induced by histamin

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01698.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

6 cases of contact allergy id the antitumor antibiotic mitomycin C from intravesical instillation arc described‐ Reports suggest that up to 9% of patients treated with mitomycin C for chemoresection/prevention of superficial bladder cancer will develop cutaneous side‐effects. Patients may present cither with vesicular dermatitis of the hands and feet and/or dermatitis of the genitals, or with more widespread eruptions, Probably most, if not all, skin reactions are caused by contact allergy. The distribution is suggestive, of systemic contact dermatitis from mitocycin C absorbed from the vesical muc

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01699.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Contact sensitivity (allergic contact dermatitis) to bis(tri‐n‐butyltin) oxide (TBTO) was demonstrated in the mouse. TBTO (in an acetone: olive oil vehicle) or acetone: olive oil alone (as a control) were applied to the shaved flank under an occlusive patch and animals were challenged on the dorsum of the car 6 days later. Ear swelling was then measured using an engineer's micrometer, 2, 24, 48 or 72 h thereafter. Significant differences in ear swelling between control and TBTO‐sensitized animals urn‐found 24 h after challenge; thereafter the elicitation reaction declined rapidly whilst irritant swelling in control animals increased. Maximal elicitation of TBTO sensitivity could only lie elicited by concern rat ions of TBTO that caused irritation. In a separate experiment, u single application of TBTO to the ears of naive animals provoked increases in auricular lymph node weight, cell yield and proliferation of lymph node cells during overnight culture. These data support the conclusion that TBTO is a contact sensitizer and illustrate the potential usefulness of the quantitative methods of contact sensitivity assessment winch have been developed in th

ISSN:0105-1873    

DOI:10.1111/j.1600-0536.1991.tb01700.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY