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11. |
Starvation‐induced programmed death of hybridoma cells: Prevention by amino acid mixtures |
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Biotechnology and Bioengineering,
Volume 45,
Issue 1,
1995,
Page 86-90
F. Franěk,
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摘要:
AbstractAssociation of the availability of nutrients with the phenomenon of programmed cell death—apoptosis—was investigated using hybridoma cells cultured in protein‐free medium under conditions of starvation, i.e., in RPMl‐1640 medium diluted to 50% with saline. Amino acid mixtures, such as MEM essential amino acids or MEM nonessential amino acids were found to prevent starvation death significantly when added to the diluted medium in 1 to 2 mMconcentrations, the MEM vitamin mixture was ineffective, and glutamine displayed a moderate growth‐supporting effect. The specific monoclonal antibody production rate in cultures supplemented with amino acid mixtures was strikingly low, whereas supplementation with glutamine alone or simultaneously with other amino acids resulted in a specific antibody production rate comparable with the rate observed in undiluted medium. © 1995 John Wiley
ISSN:0006-3592
DOI:10.1002/bit.260450112
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1995
数据来源: WILEY
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12. |
The Monod equation and mass transfer |
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Biotechnology and Bioengineering,
Volume 45,
Issue 1,
1995,
Page 91-94
J. C. Merchuk,
J. A. Asenjo,
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PDF (284KB)
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摘要:
AbstractAn alternative interpretation of the growth rate–substrate concentration dependence is presented. This is based on the assumption that the main factors affecting growth rate are transfer of substrate from the medium and the maximum growth velocity, which is that observed when no substrate limitations occur. This approach allows the approximate prediction of one of the two kinetic constants required, and may be of great use, especially for continuous cultures. It is the first attempt to provide a phenomenological explanation for the large variations observed in the values of the Monod constant,Ks, reported in the literature. © 1995 John Wiley&Sons, I
ISSN:0006-3592
DOI:10.1002/bit.260450113
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1995
数据来源: WILEY
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13. |
Separation of amino‐acid enantiomers using Micellar‐enhanced ultrafiltration |
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Biotechnology and Bioengineering,
Volume 45,
Issue 1,
1995,
Page 95-95
A. L. Creagh,
B. B. E. Hasenack,
A. Van der Padt,
E. J. R. Sudhölter,
K. Van't Riet,
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PDF (36KB)
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ISSN:0006-3592
DOI:10.1002/bit.260450114
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1995
数据来源: WILEY
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14. |
Masthead |
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Biotechnology and Bioengineering,
Volume 45,
Issue 1,
1995,
Page -
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PDF (123KB)
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ISSN:0006-3592
DOI:10.1002/bit.260450101
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1995
数据来源: WILEY
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