摘要:

AbstractFoam separation of microorganisms has been investigated with varying success by many workers, usually at high rates of gas flow. Microflotation was developed to overcome some of the disadvantages inherent in these high gas‐flow‐rate processes and is introduced in this paper as a new technique for the foam separation of microorganisms at low gas‐flow rates. With microflotation, a stable surface phase is produced by adding an insoluble collector such as a long‐chain fatty acid or amine. The formation of an insoluble surface phase eliminates the need for high foaming. Low rates of gas flow are used resulting in a more efficient separation and a less voluminous and drier surface phase upon which to collect the microorganisms. The efficiency of this technique is also improved by using flotation aids such as frothers and flocculents. Frothers are used to improve the collector properties of the surfactant and to refine further the small bubbles produced by a very fine sparger. Small concentrations of flocculents, such as alum, are used to partially agglomerate the organisms and provide sites for adsorption of collector. The work described in this paper is preliminary in nature, designed to illustrate that a low flow‐rate process may be used to separate microorganisms and to stimulate further research. The applications discussed are removal of the bacterium,Escherichia coli, and alum, and two species of algae,Chlamydomonas reinhardtiiandChlorella ellipsoidea, using stearylamine without alum. The frother used wa

ISSN:0006-3592    

DOI:10.1002/bit.260080112

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1966

数据来源: WILEY

摘要:

AbstractThe design and operation of a permanently‐piped plant for sterilizing 10–200 l. of tissue‐culture media is described. The plant is sterilizedin situby steam injection, the filtration rate is 2–3 l./min., and the turn‐around time for volumes of 100 l. is a

ISSN:0006-3592    

DOI:10.1002/bit.260080113

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1966

数据来源: WILEY

摘要:

AbstractProbabilistic models for the killing of microorganisms are formulated and described. The utility of the models is illustrated by applying them to the optimization of an idealized fermentation process.

ISSN:0006-3592    

DOI:10.1002/bit.260080114

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1966

数据来源: WILEY

摘要:

AbstractA versatile and simple continuous‐culture apparatus is described. With this apparatus, independent control of limiting growth factors and other nutrilites is possible and the conditions of each experiment are reproducible. In view of the synchronized speed of the feeder syringes, flow variation troubles are not encountered. The device allows the performance of growth experiments at different dilution rates simultaneously in a single run which makes the comparison of the results more reliable. The operation of the device has been tested successfully with a study of adenine deaminase induction in yeas

ISSN:0006-3592    

DOI:10.1002/bit.260080115

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1966

数据来源: WILEY

ISSN:0006-3592    

DOI:10.1002/bit.260080116

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1966

数据来源: WILEY

ISSN:0006-3592    

DOI:10.1002/bit.260080101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1966

数据来源: WILEY