摘要:

Gingival biopsies were taken from three main categories of patients. One group (IS) comprised 19 patients of whom 16 had received renal allografts. All were treated with immunosuppressive agents. A second group (UH) consisted of 19 patients who suffered from chronic renal failure. Control specimens were obtained from 30 systemically healthy subjects with plaque‐free teeth and healthy gingiva. Samples were also taken from 30 other systemically healthy persons with less efficient oral hygiene. Their gingival index was 1. All specimens were examined by light microscopy. In addition, eleven selected biopsies were processed for electron microscopy. Beneath the dento‐gingival epithelium the control group with plaque had a significantly higher number of cells than the other groups (p<0.05). Residual cell infiltrates were always found in the samples from the plaque‐free healthy subjects and the uremic patients, whereas scaling and an adequate plaque control virtually eliminated inflammatory cells from the IS specimens. Lymphocytes predominated in the lesions of the UH and IS patients with clinical loss of attachment and persistent inadequate oral hygiene. It is concluded that immunosuppression does not abolish the host reaction to dental plaque, but the inflammatory and/or immune responses are different from those in otherwise healthy pe

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01162.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The purpose of the present study was to evaluate the lymphoproliferative response of submandibular nodes cells to stimulation by phytohemagglutinin (PHA), concanavalin A (Con A), and lipopolysaccharide (LPS) following experimental gingivitis in dogs.In 4 dogs of the experimental group, gingival inflammation was induced by cervical ligatures on the left jaw, and the right jaw was kept clean by toothbrushing. In 2 dogs of the control group, toothbrushing was performed throughout. After the weight and lymphocyte number in the submandibular nodes were calculated, the lymphocyte response was determined by the uptake of3H‐thymidine.The weight and lymphocyte number of the ipsilateral nodes increased 2.2 and 3.7 times, respectively, as compared with the contralateral nodes. There was a higher background proliferation of unstimulated lymphocytes from ipsilateral nodes. The lymphocyte response to lower PHA concentration from ligated side lymphnodes significantly increased compared to that from contralateral nodes. It is suggested that the plaque accumulation and the intensive gingival inflammation considerably influence lymphocytes in the submandibular node

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01163.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Culture filtrates of strains ofBacteroides gingivalisandBacteroides asaccharolyticuscontain a heat stable factor which inhibits the production of extracellular matrix by chick embryo chondrocytes and which causes vacuolization of these chondrocytes. This morphological effect appeared to be reversible. Strains of the saccharolytic speciesBacteroides melaninogenicusdid not have these effects. The significance of this cytotoxic effect for the etiology of periodontal diseases is discussed.

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01164.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Longitudinal studies in this laboratory have been examining the efficacy of tetracycline HCl in the treatment of alveolar bone resorption due to chronic destructive periodontal disease in beagle dogs. Following one year of tetracycline treatment we reported a reduction in the rate of alveolar bone resorption in beagles receiving either 250 or 500 mg daily tetracycline when compared to a group of untreated control beagles. We have continued daily tetracycline treatment in either 250 or 500 mg doses in these beagles for a total of two years in order to more fully evaluate the overall efficacy of tetracycline in having an effect on the periodontal disease process. This report presents data from the conclusion of the 30 month study which was divided into a 6 month pretreatment period and a 24 month treatment period. The results indicate that tetracycline as the sole treatment modality significantly suppressed the rate of alveolar bone resorption over 18 months of the treatment period. During the subsequent six months, the rate of bone resorption in both groups of treated animals returned to baseline rate. The overall effect of daily tetracycline treatment in the 24 month treatment period, compared to a group of untreated control beagles, was the preservation of 20.6% alveolar bone in the 250 mg treated dogs and 44.2% alveolar bone in the 500 mg treated dogs.

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01165.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The purpose of the present study was to examine if streptococci, isolated from human dental plaque in experimental subjects having practiced 2 years daily oral chlorhexidine hygiene, showed any difference in susceptibility to chlorhexidine compared to plaque streptococci isolated from a control group. The minimal inhibitory concentrations (MIC) of chlorhexidine were determined using the broth dilution test tube method. From an 18 h culture of each strain in brain heart infusion, 0.2 ml were inoculated into 5 ml brain heart infusion with added chlorhexidine to final concentrations of 0, 0.5, 1, 2, 4, 8, 16, 32, and 64 μg per ml. Three months later identical tests were performed. In both tests strains ofStreptococcus mutans, Streptococcus milleriandStreptococcus salivariuswere found to be the most susceptible to chlorhexidine, showing MIC around 4 μg chlorhexidine per ml. Strains resemblingS. milleriappeared slightly less susceptible than strains identified asS. milleri. The majority of theS. sanguisstrains had MIC ranging from 8 to 16 μg per ml. This species was slightly more susceptible than a group of streptococci intermediary toS. sanguisandS. mitior, while the susceptibility of theS. mitiorstrains ranged from 4 to more than 64 μg per ml. A difference in susceptibility to chlorhexidine between strains isolated from the chlorhexidine group and the control group was only observed forS. sanguis. Strains of this species isolated from the chlorhexidine group showed higher MIC than did strains isolated from the control gr

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01166.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The purpose of this investigation was to determine the effects of stannous fluoride mouthrinses containing 250 or 1000 ppmF on plaque accumulation, gingivitis, microbial composition of plaque, and the ability of saliva to produce acid, in 48 subjects who abstained from oral hygiene for 5 days. Plaque formation was significantly lower in both SnF2groups. Clinical gingivitis and gingival fluid measurements did not differ. Significant reductions in viable plaque organisms were provided by the fluoride rinses. No change was noted in the proportions of aerobic, anaerobic, gram‐positive, or streptococcal bacterial populations. Both fluoride rinses inhibited the ability of saliva to lower the pH of a sugar solutio

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01167.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A five‐day study in 48 subjects, conducted in the absence of customary oral hygiene, measured the effects of a single topical application of stannous fluoride or acidulated phosphate fluoride alone or in a 1:1 combination. All fluoride concentrations were 1.25%. At the end of the study no effects on dental plaque and gingivitis were detected. During the study the flouride treatments, particularly those containing SnF2, temporarily restricted the ability of the saliva to lower the pH of a sucrose solutio

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01168.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Radiolabeled microspheres were used to measure gingival and alveolar bone blood flow in beagles with periodontal disease. The severity of periodontal disease was determined using radiographs, and the percent alveolar bone loss around specific tooth areas was calculated. Gingiva and alveolar bone blood flows were lowest in dogs with minimal periodontal disease (20% bone loss) had periodontal blood flows 250–400% greater (p<.05). In all animals maxillary alveolar bone had higher blood flow than corresponding mandibular bone (p<.05) whereas gingival blood flow between jaws was simila

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01169.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The effects of colchicine administration on the ultrastructure aad function of periodontal ligament fibroblasts was studied in young mice as a further attempt to document the microtubule‐dependent nature of collagen secretory granule translocation.Disruption of the normal appearance of the Golgi complex was an early response to colchicine administration. This change was visible at the light microscopic level. At the electron microscopic level it was observed that Golgi saccules were dispersed and that Golgi cisternae were shorter and reduced in number. Microtubules were absent. Dense granules accumulated in a juxtanuclear region of cytoplasm previously occupied by the normal Golgi complex. Lipid droplets and autophagosomes were increased in number. Intermediate filaments (10 nm diameter) formed perinuclear fascicles several hours after colchicine treatment.These results as well as the recently published report (Cho&Garant 1981d) support the concept that microtubules play an important role in the normal organization of the Golgi complex and in the translocation of secretory granules to the fibroblast cell surfac

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01170.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

During eruption of the mouse incisor part of the periodontal ligament moves along with the tooth in the occlusal direction and is degraded in the supra‐alveolar region.In the present study stereologic analysis at the ultrastructural level has shown that significant reduction of the amount of extracellular collagen is confined to the most occlusal region of the ligament, just apically to the functional epithelium. In connection herewith a relatively high amount of homogeneous material of moderate electron density was found in the intercellular space, suggesting a process of extracellular collagen breakdown near the epithelium‐connective tissue interface.With respect to the distribution of collagen phagocytosis by fibroblasts, it appeared that the volume density of cytoplasmic vacuoles containing collagen fibrils was considerably higher in the supra‐alveolar region of the ligament than in its subcrestal part, particularly in the zone along the tooth surface. This phenomenon is interpreted as being indicative of increased phagocytic activity of fibroblasts leading to the detachment of the periodontal ligament from the cemeatum before it enters the region of final degradation near the apical termination of the junctional epithelium.In an attempt to identify the cells involved in the degradation of (fragments of) periodontal fibroblasts in the supra‐alveolar region, periodontal ligament cells were labelled with tritiated thymidine at their site of origin. By means of both autoradiography and electron microscopy, it was shown that, following the arrival of Jabelled fibroblasts in the supra‐alveolar region, at least part of the degradation of cellular constituents is accomplished via a process of heterophagocytosis among fi

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01171.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY