摘要:

The principal aim of this work was to analyze the host systemic immune response to an oral infection ofA. viscosusT14V. Previous work bad established a Balb/c mouse model forA. viscosusT14V oral infection. Animals were infected and the splenocyte response toA. viscosusT14V antigens, LPS, and Con A were assessed in the lymphoblast assay. Splenocytes from mice infected withA. viscosusT14V exhibited an altered response pattern to the antigens and mitogens. The responses were enhanced or suppressed when compared to the uninfected control splenocyte response. The effluent celis from a nylon wool column were unresponsive toA. viscosusantigens but LPS did respond to Con A. This response pattern was true for splenocytes from infected and uninfected animals.These results suggest thatA. viscosusT14V is a potent immunomodulater when analyzed within the Balb/c mouse model system.

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01150.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Monoclonal antibodies made against lymphocyte differentiation antigens were used to characterize phenotypically the lymphoid cell subpopulations in sections of human lymphoid tissue and inflamed gingival tissue associated with the deciduous dentition in children. Four monoclonal antibodies designated FMC 1, FMC 4, FMC 7, and UCHT1 were used. These antibodies are specific for B‐cells, p28,33 (la‐like) antigen, a B‐cell subset, and peripheral T‐cells respectively. FMC 1 and FMC 4 positive cells (B‐cells) were found mainly in the secondary follicles while UCHT1 positive cells (T‐cells) were found in the parafollicular areas of human tonsils. Cells in some, but not all, secondary follicles in the tonsils were FMC 7 positive.In the gingival tissue only 12.6 % of the infiltrating cells were FMC 1 positive, 12.2 % were FMC 4 positive, and 4.8 % FMC 7 positive. On the other hand over 75 % of cells appeared to be UCHT1 positive.These results indicate that the majority of inflammatory cells in gingivitis associated with the deciduous dentition in children have the phenotype B‐cell antigen‐/p28,33 (lalike) antigen‐/B‐cell subset antigen‐ and possibly T‐cell antigen+. Such a phenotype is, at least by exclusion, suggestive of T‐cells and as such confirms earlier studies us

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01151.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The biosynthesis of the third and fifth components of complement in chronically inflamed gingiva is demonstrated. The technique utilized in these studies was an immunochemical detection of14C‐labeled amino acid incorporated protein from gingival explant cultures. C3 and C5 synthesis was detected in 8 of 10 individuals with periodontal disease, while no evidence of synthesis was noted in 5 control subjects. The results indicate an even greater consumption of complement occurs in periodontal disease than has been predicted previously using serum as the sole source of complement. The local production of complement could play a role in modulating the inflammatory response in the gingiv

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01152.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The placement of a ligature around the second maxillary molar of the conventional rat caused osteoclastic bone resorption and simultaneously, alveolar bone formation. The number of peripheral mononuclear cells in the blood and lymphoblastic transformation of spleen cells in response to concanavalin A increased. Cyclophosphamide (CY), an immunosuppressive agent, given shortly after placing the ligature suppressed the lymphoid reactions, spleen size, and bone formation and enhanced bone destruction. CY given in higher doses also suppressed the number of PMN cells. Septicemia developed in several of these animals.Pseudomonas aeruginosa, Klebsiella pneumoniaeandEscherichia coliwere isolated from the blood and/or ligature. Antibiotics prevented bone destruction. Without placing a ligature, the high dose of CY did not result in bone loss.These findings suggest that 1) bone destruction of the ligature‐treated rat is of bacterial origin, 2) CY suppresses proliferation of osteoblasts but does not seem to interfere with the activity of osteoclasts, and 3) suppression of the host defenses greatly facilitates bone destructio

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01153.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Collagenolytic activity was assessed in a variety of microorganisms with particular emphasis on members of the indigenous oral flora. Organisms were grown in complete and peptide depleted basal anaerobic broth. Cell sonicates and media preparations were assayed for collagenolytic activity using14C‐labelled collagen in solution and as fibrils. Assay reaction products were evaluated by acrylamide gel electrophoresis.All tested species of Bacteroides, including B.gingivalis, B,melaninogenicusss.melaninogenicusandintermedius, B. capillus, B. oris, B. thetaiotaomicron, andB. fragilisproduced collagenase which was primarily associated with the cell fraction. Collagenolytic activity was also observed in both media and cell sonicates ofActinobacillus actinomycetemcomitans, Strain 511. TheBacteroidesandActinobacillusenzymes were heat labile, inhibited by EDTA and human serum. Enzyme activity appeared to be enhanced when these organisms were grown in peptide depleted medium. Collagenase production by tested species of Bacteroides and A. actinomycetemcomitans (511) was unique among other members of the oral microflora including species ofFusobacterium, Actinomyces, Capnocylophaga, andSelenomonas, which did not demonstrate collagenolytic activity under the same cultural condition

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01154.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The epithelial surface of deep periodontal pockets has been described using scanning electron microscopy. The presence of microtopographically distinct areas suggests that the pocket wall is constantly changing as a result of the interaction between the host and the epithelial‐ and pocket‐associated bacteria. Seven topographical areas were described: relative quiescence, bacterial accumulation, emergence of leukocytes, leukocyte‐bacterial interaction, epithelial desquamation, hemorrhage, and ulcer

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01155.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Magnification angiography was used to visualize the mandibular vasculature of beagle dogs with either minimal or moderate to severe periodontal disease. The arteriograms were qualitatively graded with respect to numbers of vessels and vessel architecture. Arteriograms of the dogs with moderate to severe periodontal disease revealed a significant increase in vessel density and tortuosity when compared to dogs with minimal disease. The technique presented in this report provides a non‐destructive method for thein vivostudy of the vascular architecture in periodontal diseas

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01156.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Localized thermal injury was produced in the coronal part of the rat incisor periodontal ligament (PDL). The tooth was cut off at the gingival margin and its pulp removed to a depth of 9 mm from the cut edge. An electrocautery needle was then inserted into the pulp cavity, and a current of two seconds duration was applied. The thermal injury affects primarily PDL vitality, and this is manifested by an eruption slow‐down accompanied by typical histological changes. Specimens for histological examination were obtained one, three, and seven days following treatment and compared with the sections prepared from sham operated and control incisors. The mean eruption rate of the cauterized teeth was slower by about 70 % than that in the other two groups. Histological sections of the former incisors revealed well demarcated coagulation necrosis, which was especially pronounced on the first and third post treatment days. Signs of repair in the damaged areas were observed on the seventh day following treatment. The lingual periodontal lesions were infiltrated with fibroblasts and vascular tissue. In some incisors, hard tissue deposits appeared in the tooth‐related part of the PDL. The PDL of sham operated or control animals did not exhibit any changes. These experiments demonstrate that isolated damage to the PDL retards tooth eruption, supporting the hypothesis which views the PDL as the prime motor pulling the erupting incisor outw

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01157.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Orthodontic appliances, designed to tip the maxillary second and third incisors in facial direction, were inserted in 3 dogs. During a 5 month period, the incisors on the left side of the jaw were tipped to a facially displaced position. During a further 5 month period these teeth were moved back to their original position while the two incisors on the right side of the jaw were moved to a position corresponding to that previously reached by the incisors of the left side. The orthodontic appliances were then used to retain the teeth In these positions for 5 months. Teeth in three non‐treated dogs served as controls. During the study, the animals were subjected to meticulous plaque control. The animals were sacrificed 15 months after the start of the study. The jaws were removed and buccolingually oriented histological sections of the experimental and control teeth were produced. The study has shown (1) that dehiscences can be produced in the alveolar bone by tipping teeth in facial direction and that bone will reform in such defects when the teeth are moved back to their original position and (2) that such tooth movements are not necessarily accompanied by loss of connective tissue attachmen

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01158.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The aim of this experiment was to achieve support for the hypothesis that bone resorption, induced by jiggling forces, leaves a component within the supracrestal soft tissue with a capacity of reforming bone. The maxillary lateral incisors and first premolars and the mandibular second premolars in two monkeys were used in the study. Using metal pins inserted into the neighboring teeth as retainers, orthodontic elastics were stretched and placed alternately around the buccal and lingual surfaces of each experimental tooth in order to produce jiggling forces. After 5 months of continuous jiggling, when bone dehiscences were produced on the buccal aspect of the teeth, the elastics were removed. After repositioning of the teeth a split thickness flap was raised. On one side of the jaw the soft tissue within the bone dehiscences was removed. At the contralateral teeth a sham operation was performed maintaining the soft tissue within the bone dehiscences. The monkeys were sacrificed 6 months after surgery. Tissue blocks containing test and control specimens were dissected free and prepared for microscopic analysis. The length of the supracrestal connective tissue attachment and the amount of coronal bone regeneration were assessed in the histological sections. It was found that buccal alveolar bone, reduced in height by jiggling forces, regenerated after discontinuation of the forces. When the soft tissue within the buccal bone dehiscences produced by the jiggling forces was surgically removed, the coronal regeneration of the alveolar bone was markedly reduced. These observations suggest that bone resorption, induced by jiggling forces, leaves a soft tissue component with a capacity of forming bone.

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1982.tb01159.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY