摘要:

Ovariectomies were performed on 8 squirrel monkeys. Four of the animals received 0.1 mg estradiol benzoate intramuscularly 3 times per week for 4 months. One hour prior to sacrifice all animals received H3‐thymidine. Sections of vagina, buccal mucosa and gingiva were processed for hematoxylin and eosin staining and autoradiographs. Measurements were made of the basemined membrane/mm of surface length, the number of basal cells/mm of basement membrane determined and a labeling index for basal cells established.The animals which had received estradiol benzoate exhibited a thick vaginal epithelium in contrast to the other animals which showed a thin and often ulcerated epithelial covering. The vaginal epithelium of the estrogen treated ovariectomized animals had longer rete pegs, fewer basal cells/mm of basement membrane and a higher labeling index than those animals not receiving estrogen. The epithelium of the buccal mucosa was significantly different in the same direction as the vagina by all three criteria. The gingival epithelium showed no significant differences by two of the three criteri

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1970.tb00727.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

This study was designed to determine whether H3‐estradiol would localize in the oral tissues of rats. Twenty ovariectomized rats were injected subcutaneously with H3‐estradiol. After injection, oral tissue including gingivae and mucosa, submandibular gland, liver, kidney, uterus and skin were dissected, weighed and homogenized. The tissues were assayed for H3‐estradiol and 5 ml aliquots added to liquid scintillation fluid. The samples were read on a Beckman Liquid Scintillator.A series of experiments using the above technique demonstrated the following: (1) H3‐estradiol concentrated in the oral extract when a dose of 0.136 μg/100 gm body weight or greater was given. (2) The assay of oral extract contained H3‐estradiol as evidenced by chromatography. (3) One hour after injection the oral extract had the highest concentration of H3‐estradiol (212 CPM/mg of tissue) while the uterus remained low (47.7 CPM/mg) in uptake. Four hours after injection there was a statistically significant increase of H3‐estradiol in the uterus (118.5 CPM/mg but not a significant decrease of H3‐estradiol in the oral extract (131.8 CPM/mg). (4) Estrogen (Progynon)*priming for 7 days reduced the uptake of H3‐estradiol in the oral extract.Since H3‐estradiol concentrates in the oral extract, it is suggested that estrogen can influence the gingival tissues on a local cellular level. The fact that estrogen priming reduced the uptake of H3‐estradiol in the oral extract indicates that there may be some type of binding site in t

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1970.tb00728.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The present study was performed in order to study the progressive growth of vessels in a regenerating tissue and to analylze the tissue events occurring outside and inside the mature micro‐vessels immediately before sprout formation. Twenty, 10–20 months old female rabbits, weighing 2–3 kilos each were used. A modified Sandison's ear chamber was installed in each animal. Healing of a tissue defect was studied in a modified Leitz intravital microscope.Immediately after installation of the ear chamber, the center of the wound was practically devoid of cells. The venules of the bordering tissue showed signs of an acute inflammation. During the first hours there was an obvious leakage from the venules of the bordering tissue of an intravenously injected protein bound dye. Some hours later granular cells started to emigrate from the adjacent pre‐existing venules into the defect space. Six to eight hours after surgery corpuscular and plasma flow was normal in all vessels in the region of the ear chamber. During the next 35–40 hours the protein network became denser. By the end of the second day most of the granular cells of the defect had lost their amoeboid properties. The nucleus and the granules of these cells became denser and more prominent. By 48–72 hours several of the rigid granular cells disintegrated and dispersed their cytoplasmic granules into the defect. A few hours after degranulation the corpuscular flow rate of the bordering vessels increased. Erythrocytes and later granulocytes penetrated the venular walls and entered the extra‐vascular space. In the immediate vicinity of the vertices of the preformed venules, strings of red and white cells moved back and forth apparently creating well defined tubes bordered by a fibrillar structure. The cell movements seemed to open up and elongate the tubes. Between the moving cells and aggregated cells of the defect a layer of thin transparent cells could eventually be detected. In this situation there were also optically definable openings in the walls of the borderline venules and true communicationsestablished between the preexisting vessels and the

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1970.tb00729.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

This study present an ultrastructural characterization of the prospective gingival epithelium in the human embryo during the third and fourth months of development.The surface cells were squamoid and interdigitated. The central cell layer was composed of more rounded cells while those of the basal layer were cuboidal to low columnar. The intercellular spaces were fewer and smaller toward the free surface. Differences in the number, variety and organizational aspects of the junctional complexes were noted relative to level of location. Hemidesmosomes were observed at the basal cell‐connective tissue interface. Pronounced differences in the kinds and number of organelles were noted at the various epithelial levels. In the surface cells, glycogen was accumulated throughout the cell and organelles were reduced in number. As the glycogen diminished in the cells toward the basal region, the organelle complement increased. The arrangement of tonofilaments tended to change toward the superficial layers. The larger masses were located in the deeper and central cells while surfaceward, tonofilaments were diffusely distribute

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1970.tb00730.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The freeze‐sectioning technique of Ullberg (1954, 1958) was tested for enzyme histochemical studies of molar eruption in the mouse when cutting the tissues in a cryostat. The method was found applicable for the purposes intended, not only in lactate and malate dehydrogenase recording but also, and especially, in those for glucose‐6‐phosphate dehydrogenase and adenosine‐triphosphatase activities in calcified tissues. The advantages and disadvantages of the technique, were discussed as compared with the difficulties of EDTA‐demineralization. Marked oxidative and hydrolytic enzyme reactions were observed in the reduced dental epithelium even in progressed stages of molar crown eruption. Hard‐tissue forming cells revealed cytoplasmic ATPase activity as well as endothelial cells and connective tissue regions beneath heavily keratinized and unstained oral epithelium. The cervical migration of the oral epithelium, after the fusion with the reduced dental epithelium, could be discerned by the different distributions of enzyme histochemica

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1970.tb00731.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1970.tb00732.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The nature of Civatte bodies in oral lichen planus lesions of 11 patients was investigated by light and electron microscopy. The results suggested that these bodies were derived from keratinizing epithelial cells which formed discrete masses of tonofilaments. These masses appeared to be engulfed by cells containing structures morphologically similar to premelanosomes, melanosomes and melanin granules of the elongated and rounded types. Degenerating mast cells may also contribute to the formation of eosinophilic masses, but these may be distinguished from the previous type by the presence of metachromatic granules.

ISSN:0022-3484    

DOI:10.1111/j.1600-0765.1970.tb00733.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY