ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00615.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

ABSTRACTMetabolism in fruit, as in all forms of life, encompasses the maintenance of cellular integrity and responsiveness to stress. It is proposed that this homeostatic tendency may, when combined with improved control (genetic, molecular) of ontogenetic development, provide a basis for prolonged storage life. Homeostasis may also be the underlying cause of increased metabolic activity in ripening tissues as manifested by the respiratory climacteric.

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00616.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00617.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00618.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00619.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

ABSTRACTYolks from fresh eggs produced by Broad Breasted White turkeys were blended and analyzed. Proximate composition was determined and components were separated in 7% polyacrylamide gel. Turkey egg yolk was compared with chicken egg yolk.Turkey egg yolk contained a higher percentage of solids, fat, and protein than chicken egg yolk. When separated electrophoretically, components of chicken egg yolk migrated faster than those in turkey egg yolk. Turkey yolks could be resolved into 10 or 11 distinct components; chicken yolk, into 11 or 12 components, depending on electrophoretic technique.

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00620.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

ABSTRACTThe inhibition of purified bovine erythrocyte acetylcholinesterase (AchE) and horse serum cholinesterase (ChE) by α‐chaconine was found to be a mixed‐type with inhibition constants (Ki) for both enzymes of 8.3 × 10‐6M and 4.0 × 10‐4M, respectively. Kinetic constants for AchE obtained for the hydrolysis of acetylthiocholine iodide averaged 7.2 × 10‐5moles/liter/min (Vmax) and 6.2 × 10‐5M (Km) whereas for ChE these were 3.8 × 10‐4moles/liter/min (Vmax) and 1.3 × 10‐4M (Km) for the hydrolysis of butyrylthiocholine iodide.Subcellular acetylcholinesterase activity in nuclear‐free rat brain homogenate showed the highest activity to be equally distributed between the mitochondrial and microsomal fractions, with only little activity in the nuclear fraction. Inhibition of the rat brain subcellular enzyme activity in vitro by 0.016 M α‐chaconine was 43% for whole homogenate, 55% for nuclear fraction, 35% for mitochondria, and 33% for microsomes.In vivo administration of α‐chaconine, (10, 30 and 60 mg/kg) to adult male rats caused a reduction of brain AchE activity to 79, 55 and 18% of the control activity for the respective doses. Heart and plasma AchE activity of treated animals did not show a dose‐related inhibition similar to that of brain. Inhibition of heart AchE was 61%, while that of plasma was 51% for rats administered to 10 mg/kg and no further inhibition for rats given 30 mg/kg α‐chaconine was noted.Isoenzymes of rat brain cholinesterases, separated by acrylamide gel electrophoresis, were inhibited by 30 and 60 mg/kg α‐chaconine administered intraperitoneally. Electrophoretic separation of plasma from the treated animals resulted in five anodally migrating zones that hydrolyzed acetylthiocholine and α‐naphthylacetate. Inhibition of the activity of isoenzyme bands 1 and 2 was 40 and 77% respectively for animals given 10 mg/kg α‐chaconine, while rats given 30 mg/kg showed inhibition of 100 and 75%, respectively. Isoenzyme bands 3 and 4 were completely inhibited in the plasma of alkaloid treated animals.In vitro inhibition of rat plasma, erythrocyte and brain esterase isoenzymes was estimated by incubating gels with 10‐4M α‐chaconine after electrophoretic separations. The slower‐moving isoenzyme bands were inhibited completely, while the faster migrating isoenzyme bands in plasma and erythrocytes were leas

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00621.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

ABSTRACTRelatively large amounts of rishitin and lubimin accumulate in potato tuber slices following treatment with actinomycin D (AD). Optimum elicitation of these terpenes occurred at an (AD) concentration of μM. Terpene accumulation was evident within 24 h after treatment and progressed for up to 96 h at which time rishitin levels exceeded 100 μg/g fresh weight, discs. Slices prepared from tubers previously held at 25°C were less responsive to (AD) induction of terpenes than those from tubers previously stored at 4°C. Treatment of discs with 50 μM cycloheximide following (AD) treatment blocked further accumulation of terpenes indicating a requirement for continual protein synthesis. (AD) (20 μM) treatment caused a slight reduction in protein synthesis for the first 48 h following treatment after which it caused an increase in protein synthesis compared to controls. (AD) also affected respiration of discs in a manner which paralleled its effect on protein synthesis. Cyanide resistant respiration was more pronounced in (AD) treated discs than control discs. The data are discussed with reference to the mechanism by which (AD) elicits terpene biosynthesis in potato tubers

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00622.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Book Reviews in this Article:Chemical and Biochemical Applications of Lasers, Volume 111.Edited by C. Bradley Moore.Methods in Enzymology, Vol. XLVII. ENZYME STRUCTURE (Part E).Edited by C. H. W. Hirs. Division of Biological Sciences, Indiana Univ., Bloomington and Serge N. Timasheff, Graduate Department of Biochemistry.Methods in Enzymology, Vol. XLVIII, ENZYME STRUCTURE (Part F).Edited by C. H. W. Hirs. Division of Biological Sciences, Indiana Univ., Bloomington, and Serge N. Timasheff, Graduate Department of Biochemistry, Brandeis Univ., Waltham, Mass.Membrane Proteins and their Interactions with Lipids.Edited by Roderick A. Capaldi.Chromatography of Synthetic and Biological Polymers, Vol. 2, Hydrophobic, Ion‐Exchange and Affinity Methods.Edited by Roger Epto

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00623.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Sept. 28, 1979: PRACTICAL APPLICATIONS OF MICROWAVE ENERGY SYMPOSIUM. K‐State Union, Kansas State University, Manhattan, sas. Contact Dr. D. Y. C. Fung, Chairman, or Dr. F. E. Cunningham, Co‐chairman, Call‐Hall, Kansas State University, Manhattan, Kansas 66 506. Telephone: 91 3‐53

ISSN:0145-8884    

DOI:10.1111/j.1745-4514.1978.tb00624.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY