摘要:

Several skin diseases may present as vesicles or bullae. Immunofluorescent studies arc very helpful in differentiating the various disease entities. Familiarity with the procedure and the various kinds of patterns that are specific and non‐specific is essential for the practicing dermatologist. Imminofluorescent patterns are particularly helpful in differentiating pemphigus, bullous pemphigoid, cicatrical pemphigoid,herpes gestationis, dermatitis herpetifomis, linear IgA dcrmatosis and porphyria. Immunofluoiescent studies of the skin and sera of these patients were vital to an understanding their pathogenesis. Imminoelectron microscopy has further helped in delineating the exact sites of immunoglobulin deposition, and, thus, identifying the location of the antigens. In pemphigus, studies at the molecular level reveal that the basic pathological process is mediated by an anti‐cellular cement substance antibody. The binding of this antibody at the cell surface level results in a process that is seen at the light microscopy level in the form of acantholysis. In bullous pemphigoid cells may play an important role. It appears that the mast cell, eosinophil. and the lymphocyte in harmony with the poly‐morphonuclear leucocyte work together to bring about an enzymatic degradation of the basement membrane. The specific role of the anti‐basement membrane zone antibody is also under current study. With advances in molecular immunology, especially monoclonal antibodies and gene technology, it is hoped that these cellular and molecular interactions will be better understood and further

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00374.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

A study of dermatopathological specimens exhibiting parakeratosis has been made and the parakeratosis classified on morphological and topographical criteria. Twelve patterns of parakeratosis have been identified and their association with particular pathological conditions described.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00375.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Sixteen hairless mice were studied by transmission electron microscopy to determine the ultrastructural changes in epidermal burner restoration following topical applications of dimethyl sulfoxide (DMSO) to hack skin. Samples of experimental skin were examined at 30 min, 1 h, 2 h and 3 h after the initial DMSO treatment and compared with that of control animals. At earlier time periods (30 min‐1 h) the DMSO treated epidermis showed greatly expanded intercellular spaces, disrupted desmosomal attachments, and desmosomal remnants lying free within the intercellular space. Mitochondria contained droplets of lipoid material which reached maximum size in the spinous and granular layers. Cytoplasmic vacuoles were particularly prominent in the 30‐min samples. Large numbers of membrane coaling granules (MCG) emerged in the 1 h specimens. The MCG were laden with numerous electron‐dense inner membranes which crisscrossed at various angles. Many of the MCG were observed in the process of fusion with plasma membranes of granular cells adjacent to the stratum corneum. At 2 h a process of recovery from the effects of DMSO was evident and at 3 h there was little or no difference between experimental and control epid

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00376.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Ulceration is an indicator of unfavourable prognosis in malignant melanoma (MM). But why do melanomas ulcerate?. Possible causes of ulecration were investigated in a group of 69 ulcerated and 69 non‐ulcerated malignant melanomas. A significant correlation (P<0.001) between ulceration and mitotic index was found. In a group of 69 ulcerated malignant melanomas, 49 had over 7. 15 had 4–6, and only 5 had 0–3 mitotic figures per 10 high‐power fields. Among 69 non‐ulcerated melanomas, 28 had more than 7, 24 had 4–6, and 17 had 0–3 mitotic figures per 10 high‐power fields. This study supports the hypothesis that ulceration of melanomas is usually the result of the destruction of the epidermis by the proliferating neoplastic cells or modification of blood supply due to the expansile activity of the tumor. The relationship between ulceration and mitotic activity helps clarify the prognostic significanc

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00377.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

A 73‐year‐old woman had a walnut‐sized tumor in I he occipital region for 10 year. The growth consisted of multiple small and large cystic epithelial masses. The cyst wall exhibited trichilemmal keratinization. Numerous squamous eddies as well as many glycogen‐rich cells were present in the cyst wall. Some small round keratohyalin granules were observed in the cells below the horny layer. The desmosomes and masses of lipid droplets were seen in the horny layer. The findings described above are similar to those observed in the follicular isthmus and the follicular infundibulum. Other interesting findings include the presence of intranuclear inclusions of tonofilament‐like material and/or keratohyalin‐like granules within the cells near the mid‐layer of

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00378.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The components of the thready material in skin lesions ofpseudoxanthoma elasticum, recessive Type I, were analysed. Immunoelectron microscopic observation revealed the presence of fibrinogen in the thready material. Part of the fine fibrils in the thready material was sensitive to collagenase. The thready material was also stained by ruthenium red. These results suggest that the thready material is composed at least of fibrinogen, collagenous protein and glycoprotein.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00379.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Improved ruthenium red staining has been applied to normal human skin andlichen myxoedematosusskin lesions for the detection of the fine structure proteoglycan aggregates. In 4 samples of normal skin and in a patient withlichen myxoedematosus, the typical fine ultrastructures of proteoglycan aggregates, i.e., many short irregularly‐curled filaments of varying lengths attached to long and rather straight filaments of hyaluronic acid were shown. Althoughlichen myxoedematosusis thought o be due to the faulty formation of proteoglycan aggregates, we suggest a different view, that this improved method of ruthenium red staining is useful for the detection of proteoglycan aggregates, and provides a better preparation for electron microscop

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00380.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

A patient with characteristic clinical, histopathological, ullrastruclural and family history of cpidermolysis bullosa simplex (EBS) developed large acquired nevocytic at the sites of some healing blisters. An isomorphic reaction may have initiated the development of these ncvi. Such large acquired nevi should be considered in the differential diagnosis of large and giant congenital nevi which have the potential to evolve into malignant melanoma.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00381.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The surface structure of altered elastic libers and elastotic material in solar elastosis has been described using a scanning electron microscope. There were 3 different areas in the dermis, depending on the degree of changes in elastic fibers: areas of (1) normal‐looking fibers, (2) thick, cylindrical fibers, and (3) a large mass of markedly changed fibers. They were mainly located in the deep, middle, and upper dermis, respectively. The normal‐looking fibers were the same as the normal elastic fiber. The thick, cylindrical fibers showed various findings ranging from relatively normal to heavily damaged surface. The large mass was composed of a cord‐like element and a fine net‐like one. There was a transition between the thick, cylindrical fibers and the large mass. It is suggested that the thick, cylindrical fiber is an early event and the mass is more damaged, as one of the elaslolic changes in solar elastosis. It is also suggested thai the thick, cylindrical fiber corresponds to the fibrous form ami the mass to the amorphous form of the elastotic m

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00382.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Five patients with mclanoacanthonia are reported. The skin lesions consisted of a single, round plaque measuring several centimeters in diameter. Histologic findings were characterized by formation within an acanthotic epidermis, of many islands of small basaloid cells and highly dendritic melanocytes. Electron microscopic examination of one lesion revealed a disturbance of melanin transfer from the highly dendritic melanocytes to the neighboring keratinoeytes.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1984.tb00383.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY