ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01539.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01540.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Recent observations indicate that stem cells of the murine hair follicle exist exclusively as a subpopulation of relatively undiffer‐entiated outer root sheath cells located in the bulge region at the mid‐portion of the follicle. Because it has been hypothesized that stem cells of interfollicular epidermis may represent targets of cytotoxic responses in acute graft‐versus‐host disease (AGVHD), we studied murine AGVHD and observed sequential skin biopsies for the presence and evolutionary pattern of follicular injury. Highly purified subsets of donor T cells were used to produce AGVHD to multiple minor histocompatibility (H) antigens in two strain combinations of mice matched for the major histocompatibility complex (MHC). In the C3H.SW‐>B6 strain combination, only CD8+ effector cells produced histologic evidence in skin of AGVHD, which peaked three weeks post‐transplant. In the B10.D2‐>DBA/2 strain combination, CD4+effector cells, and to a lesser extent, GD8+cells, mediated disease, which peaked during the fourth week posttransplant. Analysis of skin from both strain/effector cell combinations revealed follicular infiltrates preferentially involving follicular stem cell (FSC) regions (bulge) of anagen follicles between the second and third weeks post‐transplant. These infiltrates often preceded infiltration of adjacent interfollicular epidermis and were associated with follicular involution to telogen (resting) phase. By the fourth week post‐transplant,>50% of follicles were in telogen phase and residual inflammation was minimal. This provided a unique opportunity to observe follicular recovery from telogen. This involved formation of buds of premordial hair matrix derived from FSC populations of the bulge regions, and subsequent descent of these buds, initially tethered by elongate columns of putative stem cell progeny, along the course of fibrous tracts corresponding to follicular sheaths. These findings suggest that stem cells of hair follicles, as well as epidermis, represent potential targets of cytotoxicity in AGVHD. Moreover, they provide in vivo demonstration that the newly‐described FSC population of the follicular bulge give rise to germinative matrix epithelium upon patho

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01541.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

HMB‐45 is a monoclonal antibody directed against human melanoma cells and which stains epidermal and dermal melanoma cells, the junctional components of common and dysplastic mel‐anocytic nevi, and melanocytes in fetal skin. In addition, melan‐ocytes in a variety of reactive conditions have been shown to label with HMB‐45, as have dermal melanocytes within Spitz and dysplastic nevi. No melanocytes in normal adult epidermis or in the dermis of common nevi have stained with HMB‐45. In order to better understand the properties of this antibody, and of the melanocytes that react with it, we stained cultured human melanocytes grown in a variety of conditions. Melanocytes from human foreskins were grown for 2–3 weeks in MCDB 153 medium supplemented with insulin, epidermal growth factor, and bovine pituitary extract as a mixed population of keratinocytes and melanocytes. Some cells were transferred to basal medium MCDB 153 (unsupplemented) for periods ranging from 3–5 days, and a subset of these were returned to growth‐factor supplemented medium. In all cases, SI00 staining was used to confirm the presence of melanocytes. Melanocytes grown in complete medium showed strong granular cytoplasmic staining with HMB‐45. Cells transferred to basal medium showed a markedly diminished staining intensity which was reversible within 3 days upon return of the cells to complete medium. The findings suggest that expression of the protein recognized by HMB‐45 may be related to a growth factor present in complete medium, but missing from basal MCDB 153. Additional studies, supplementing medium with phorbol esters, markedly enhanced HMB‐45 staining and essentially removed keratinocytes from cell cultures. As determined by measurement of melanocyte proliferation, staining of HMB‐45 did not correlate with mitogenic activation but may correlate with biochemical activation by

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01542.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Two cases of sebaceous carcinoma (SC), as well as epithelial tumours, melanoma, and lymphoma, were examined using immunoperoxidase and a panel of monoclonal antibodies on cryostat sections. The results showed that, whereas all SC cells in both cases reacted strongly with monoclonal antibody OK.M5, other tumour cells (except juvenile xanthogranuloma cells) did not. The pagetoid cells within the epidermis of SC also reacted with OKM5 antibody. Although the nature of the phenomenon merits further study, this reactivity, or cross‐reactivity, might possibly aid diagnosis of S

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01543.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

A case of transient bullous dermolysis of the newborn is reported. A healthy Hispanic newborn developed bullae during the first two days of life. From age two to four weeks, the lesions healed with milia formation. There was no residual scarring or hypopigmentation. An induced blister showed dermal‐epidermal separation with the PAS‐positive basement membrane in the epidermal roof. PAS‐positive inclusions were present in the cytoplasm of a few basal cells. Examination by electron microscopy showed degeneration of the collagen and anchoring fibrils. There were numerous stellate inclusions in the endoplasmic reticulum of the cytoplasm of the lesional basal keratino

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01544.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Three cases of neonatal lupus erythematosus (NLE) syndrome are described. The key diagnostic sign is the erythematous and annular clinical appearance of lesions. The histopathologic findings may be subtle in NLE syndrome compared with discoid or subacute cutaneous lupus erythematosus. Direct immunofluo‐rescence testing may be useful in supplementing the histopathologic evaluation. Detection of immunoglobulins, complement, or both at the basement membrane zone in lesional skin by immunofluorescence occurs in approximately half of cases; a negative study does not preclude the diagnosis of NLE syndrome. Clinical and serologic evaluation of both the infant and the mother are important in establishing a diagnosis of NLE syndrome, particularly when histopathologic findings are subtl

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01545.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

An adnexal tumor with features of both clear cell hidradenoma and mucinous syringometaplasia is reported. The lesion occurred as a solitary, asymptomatic, slow growing nodule of 6‐months duration on the anterior neck of a 55‐year‐old woman. Serous fluid could be expressed from a central skin opening. Histologically, there was a squamous‐lined imagination with foci of mucin‐ladcn cells. A ductal component with a variable admixture of mucinous cells merged with a nearby clear cell hidradenoma. Because clinicopathologic similarities exist between mucinous syringometaplasia and clear cell hidradenoma with mucinous change, thorough sectioning of mucinous syringo‐metaplasia‐like lesions is necessary to exclude an associated sweat g

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01546.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

We report a case of localized epidermal necrolysis that developed 24 hours after an intravenous injection of vinblastine. Clinically, the lesions consisted of erythematous macules, vesicles, and bullae with a linear arrangement over the injected vein. Histologically, the lesions showed features closely resemr bling erythema multiforme (epidermal necrolysis). We discuss, the pathogenesis of this curious cutaneous drug reaction and review the literature concerning local cutaneous complications associated with intravenously administered chemotherapeutic agents.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01547.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

An asymptomatic 24‐year‐old woman presented with multiple discrete papules on the extensor surfaces of the hands and wrists. Light microscopy revealed focal increase in the amount of dermal fibroblasts as well as deposition of hyaluronidase‐labile mucoid substance. The collagen and elastin were decreased. The changes were consistent with acral persistent papular mucinosis (APPM). In electron microscopy, the intercellular glycosaminoglycans showed small ruthenium red‐positive granules and thin filaments indicating normal morphology. The fibroblastic cells, however, were conspicuously altered. Endoplasmic reticulum was dilated, cytoplasm contained large amounts of osmiophilic, concentric lysosomal structures, and there was distinct fibrous lamina in the nuclear membrane. It was concluded that the primary event in APPM probably affects the intracellular metabolism of the dermal fibroblast. The accumulation of lysosomal structures may be a distinct feature of APPM differentiating it from the other reactive cutaneous muci‐noses, or it may only reflect nonspecific degeneration in a longstandi

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01548.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY