摘要:

A series of 25 cutaneous B‐cell lymphoid proliferations was analyzed for the presence of the (14;18) translocation using the polymerase chain reaction, functional sequences of rearranged chromosomes 14 and 18 were amplifiedin vitro, and t (14;18) specific sequences were detected in 1 of 14 primary cutaneous B‐cell lymphomas, in 1 of 14 primary nodal B‐cell lymphomas and in none of 3 B‐cell pseudolymphomas. These results indicate that the t (14;18) may occur in a small subset of primary cutaneous lymphoma. However, the difference in incidence of the t (14;18) between primary nodal and primary cutaneous lymphomas suggests that different molecular mechanisms are involved in the pathogenesis of these ly

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00605.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

We report 14 cases of trichogerminoma, a rare form of cutaneous adnexal neoplasm, derived from hair germ epithelium. The neoplasm occurred in 9 men and 5 women. Their ages ranged from 16 to 73 years (median 53 years). The tumors were slow growing, asymptomatic dermal or subcutaneous nodules, located on the head and neck (6), trunk (4), extremities (2) and hip (1), with no distinguishing clinical features. Histologically, trichogerminomas were characterized by sharply circumscribed, pseudo‐encapsulated dermal and subcutaneous nodules, ranging in size from 0.4 to 4.0 cm in diameter (mean 1.9 cm). The nodules were subdivided into lobules separated by variable amounts of stroma that demonstrated varying cellularity and mucin content. The lobules were composed of basaloid cells that formed densely packed, round nests or “cell balls” resembling hair bulbs. The basaloid cells demonstrated peripheral palisading, keratinization and differentiation towards various pilosebaceous structures. Retraction spaces, well developed hair follicles and hair shafts were not observed. These distinctive histologic features separated these neoplasms from other tumors of pilar origin and from basal cell carcinoma. The trichogerminomas behaved in a benign fashion with one exception. Complete excision of the lesions is the treatment of c

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00606.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Mast cell numbers were quantitated in adult cases of mastocytosis demonstrating non‐diffuse perivascular and upper dermal concentrations of mast cells. Using the Leder stain and computerised video image analysis, a mean of 382 (± 28 SE) mast cell per mm2were counted in the superficial dermis in skin biopsies from 30 adult cases of mastocytosis, in contrast to a mean of 43 (± 5 SE) mast cells per mm2in skin biopsies from 50 inflammatory dermatoses represented by subacute dermatitis, pigmented purpuric dermatosis, erythema multiforme, lichen planus and granuloma annulare. Ten skin biopsies showing no significant inflammation had a mean of 54 (± 7 SE) mast cells per mm2in the upper dermis. The mean area of individual mast cells as assessed by image analysis in the mastocytosis group was 47.40 um2(± 2.26 um2, SE) which was significantly different (P<0.01) than the mast cell area (32.34 μm2± 2.22 μm2, SE) in all other groups combined. Computerised video image analysis represents an alternative technique which is useful in assessing mast cell numbers and particularly mast cell size in adult cases of macular mastocytosis and in other de

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00607.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The percentage of keratinocytes in the proliferative phase of the cell cycle (S + G2+ M) was measured by DNA How cytometry in sun‐exposed, non‐exposed, and tretinoin‐treated skin. Before tretinoin treatment, the percentage of keratinocytes actively cycling was higher in sun‐exposed than in non‐sun‐exposed skin (p = .002) and was correlated with clinically assessed photo‐damage (p = .007). Subsequently, tretinoin‐treated sun‐exposed skin was compared to the pre‐treatment sun‐exposed skin. Overall, there was no statistically significant change. However, there was a trend toward a decrease1 in the percentage of keratinocytes in the S + G2+ M phases immediately after four months of tretinoin us that was limited to the most severely damaged patients. This effect was no longer evident two months after discontinuing treatment. This is the first study, to our knowledge, utilizing flow cytometry to investigate the effects of tretinoin in patients with varying d

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00608.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Bullous pemphigoid (BP) antigen and α6 integrin are hemidesmosome‐associated glycoproteins of basal keratinocytes. In this work, the immunoreactivity of antibodies to BP and to α6 in salt‐ or dispase‐split human skin, and in 46 biopsy specimens of various genetic and autoimmune bullous dermatoses taken from various body sites, was studied by double‐labeling immunofluorescence. In all specimens, both antigens localized at the same side of the blisters observed, i.e. the roof of the bulla in cases with a junctional or hemolytic cleavage, or the floor of the blister in those with intraepidermal cleavage. Immunostaining for α6 was strong and present in all specimens studied, whereas the one obtained with the BP serum was absent from some specimens. These results show that the BP antigen and the α6 integrin colocalize at the level of cleavage in bullous diseases; however, the more consistent and reproducible reactivity obtained with the anti‐α6 antibody suggests that this should be preferentially used in the immunohistochemical investigation of bull

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00609.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Light and electron microscopy and indirect immunofluorescence techniques were used to study the nature of the mitten deformity in live adult patients with severe generalized recessive dystrophic epidermolysis bullosa undergoing release of hand and finger contractures. Although the mitten appeared largely to be clinically separated from the underlying fixed digits, histology showed mostly normal keratinocytes beneath a thickened stratum corneum. The lower margin of the milieu was formed just below the lamina densa of the basement membrane, at a level similar lo dial of the usual blister formation in this condition. No anchoring fibrils and only a few distinct dermal structures were noted. A substantial portion of the mitten, however, consisted of necrotic keratinocytes without an intact basement membrane. This finding suggests that the mitten is not suitable for use as an epidermal autograft and confirms the rationale for taking split thickness skin grafts to close skin defects in patients with recessive dystrophic epidermolysis bullosa undergoing plastic surgery.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00610.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Image analysis of histologic sections of II patients with clinical Stage 1 melanoma, 1.00 mm – 2.50 mm, who developed metastasis, was done to determine the significance of lymphocytic infiltrates relative to metastasis and survival. An ago, sex, site, and thickness matched control group of non‐metastasizing clinical Stage 1 melanoma revealed no significant difference in the lymphocytic infiltrate parameters from the metastasizing group with the exception of the ratio of lymphocyte infiltrate width lo the tumor width (p = 0.003). Increased lymphocytic infiltrates within the tumor and subjacent lo its base significantly correlated with delayed lime to metastasis (p = 0.014 and p<0.001, respectively) and longer survival period (p = 0.045 and p<0.001, respectively). Lymphocytic infiltrate area at the tumor base in relation to tumor area was of prognostic value: the larger the ratio, the greater the time interval from metastasis to death (p = 0.0

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00611.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Factor XIIIa+and CD34+dendritic cells, believed to be subsets of monocyte/macrophages, have been identified in dermis and in dermal tumors. The purpose of this study was to determine the presence and distribution of analogous cell types in oral sub‐mucosa and oral fibro‐vascular lesions. Antibodies to XIIIa, CD34, S‐100 protein, and macrophage antigen (MAC 387) were tested on formalin‐fixed, paraffin‐embedded tissue sections from normal mucosa, peripheral fibroma (PF), peripheral ossifying fibroma (POP), peripheral giant cell granuloma (PGCG), pyogenic granuloma (PG), lymphangioma (La), benign fibrous histiocytoma (BFH), idiopathic histiocytosis (IH), angiofibroma (Af) using an ABG immunoperoxidase technique. Numbers of positively stained cells were compared to unstained cells in the tumors. XII la positive submucosal dendrocytes (GD34‐, S‐100‐, MAC 387‐) were found in abundance in normal tissue in characteristic distributions: collagen‐associated, vessel‐associated, and lymphoid‐associated. The percentage of XIIIa+cells in the oral tumors was as follows: PF: 10–30%, POF: 5–10%, PGCG: 0–5%, PG: 5–20%, La: 0%, BPH: 5–25%, IH: 0%, and Af: 10–20%. CD34+dendrocytes (XIIIa‐, S‐100‐, MAC 387‐) were few in number and were found in deeper submucosa, especially around skeletal muscle. Other than blood vascular endothehum, CD34+cells were not generally seen in the oral tumors studied. It is concluded that two previously unrecognized dendrocyte populations reside in normal submucosa. XIIIa+cells participate in the formation o

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00612.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Characteristics of keratins of live carcinomas of sweat gland origin were immunohistochemically investigated with several antikeratin monoclonal antibodies with differing specificities. Specimens were obtained from two cases of mucinous carcinoma of the skin, two cases of classic type of eccrine adenocarcinoma, and a case of eccrine porocarcinoma. The tumor cells of mucinous carcinoma expressed only simple epithelial keratins. In a case of eccrine adenocarcinoma, simple epithelial keratin 19 was diffusely expressed. The expression of the other simple epithelial keratins was confined to the luminal cells, whereas the remaining tumor cells further expressed stratified epithelial keratins. Eccrine porocarcinoma and a second case of eccrine adenocarcinoma did not express simple epithelial keratins, although stratified epithelial keratins were diffusely expressed. These data suggest that carcinomas of sweat glands express various combinations of simple and stratified epithelial keratins. Development of additional data along these lines may help to further define their classification.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00613.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The cytokeratin expression of seborrheic keratosis was studied by means of immunohistochemistry and compared with that of normal human skin. The following findings were obtained in seborrheic keratosis: (1) a partial lack of high molecular weight cytokeratin (#1/68 kD, # 10/56.6 kD) in all ten cases examined; (2) the detection of cytokeratin typical for simple epithelia (#8/52.5 kD, # 18/45 kD, # 19/40 kD) in eight often cases; and (3) the detection of cytokeratin #5/58 kD in supra‐basal cells in 5 of 10 cases. An immunoelectron‐microscopic investigation, using an anti‐keratin antibody against cytokeratin #19/40 kD, revealed a whirl‐like arrangement of keratin filaments within immunoreactive cells, in contrast to a linear, parallel arrangement in non‐immunoreactive cells. Cells known to express cytokeratin typical for simple epithelia, such as sweat gland cells or Merkel cells, were not observed. The altered cytokeratin gene‐expression in seborrheic keratosis may be attributable to de‐differentiation of tumor cells or potential re‐differentiation towards embryoni

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1992.tb00614.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY