ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01143.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The application of immunostaining techniques to electron microscopy specimens has led to a renewal of interest in electron microscopy in biological research in general, as well as in dermatopathology. Refinements in the preparative procedures have made easier the immunolocalization of antigens both in chemically‐fixed and frozen unfixed tissues, embedded in plastic and sectioned. Application of these methods has led to the demonstration of the bullous pemphigoid antigen inside basal keratinocytes. HMB‐45 antigen has been found to be present in premelanosomes. Recently, Factor XIIIa has been localized not only in dermal dendrocytes but also in endothelial cells and mast ce

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01144.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Proteins involved in the formation of desmosomes and simpler adherens junctions were studied in three types of non‐immune acantholytic diseases; specifically, four cases of Grover's disease (GD), one case of Hailey‐Hailey's disease (HMD) and one case of Darier's disease (DD), and these were compared to two cases of immune‐mediated acantholytic disease pemphigus vulgaris (PV). The proteins studied included: 1. The intracellular desmosomal proteins, desmoplakin I and II and plakoglobin; 2. The intercellular desmosomal proteins, desmoglein and CD44; and 3. vinculin, which is a major intracellular protein of the simpler aherens junctions. In GD, HHD and DD, immunostaining showed a loss of desmoplakin I and II and plakoglobin from the desmosomes, and a diffuse staining in the cytoplasm. In contrast, in pemphigus vulgaris, these proteins seemed intact and were localized to dot‐like spots on the cell surface. Also, desmoglein, and CD44 were slightly affected in GD, and moderately affected in HHD and DD. Absence of desmosomal attachment plaques, the lack of labeling with desmoglein in the affected desmosomes and a diffusion of the labels into cytoplasm were demonstrated with electron microscopy using an immunogold technique. In PV, desmoglein III is one of the target antigens for the autoantibodies in this disease and was only partially preserved in a small number of lesional cells, while CD44 was mostly preserved. Vinculin was intact in GD, HHD and DD, but was lost in PV. This study, our previous work, and that of others, suggest that: 1. In GD, HHD and DD, the proteins of the desmosomal attachment plaque are primarily affected; 2. In PV, the intercellular glycoproteins are primarily involved; and 3. Simple adherens junctions are intact in GD, HHD and DD, but are damage

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01145.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The term “nevoid malignant melanoma” (nevoid MM) is used here to describe rare nodular malignant melanomas that may escape detection in routine histological sections due to the lack of a prominent intraepidermal component, sharp lateral circumscription and evidence of partial maturation with descent in the dermis. Nevoid MM mimic ordinary compound or intradermal melanocytic nevi when the melanoma cells are small, or Spitz's nevi when the cells are large.The patterns of HMB‐45 staining in 12 nevoid MM were compared with those in 107 melanocytic nevi. HMB‐45 staining was strong in the dermal component of the nevoid MM, even in the absence of a junctional component. In common acquired and congenital nevi, the upper dermal component stained less than the junctional component of the lesion. The deepest components of these nevi were negative. Spitz nevi and cellular blue nevi had positive dermal cells, even without a junctional component. Additional staining for a proliferation marker, such as cyclin (PCNA) or Ki‐67 (with the antibody MIB‐1), can help further in distinguishing a nevoid MM from a Spitz's nevus. Melanoma has strong nuclear staining throughout the lesion. In contrast, Spitz's nevi have more staining at the top of the lesion than at the bottom. The patterns of HMB‐45 and MIB‐1 staining can be used along with standard histologic criteria for the diagnosis of nevoid MM. Clinicopathologic correlation is needed to distinguish some metastatic melanomas from pri

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01146.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Applying immunohistochemical procedures for the detection of eight different cytokeratin (CK) polypeptides and other differentiation markers, we compared the staining patterns of normal cutaneous structures with those of benign adnexal tumors (n=65). Syringomas exhibited a marker pattern highly reminiscent of that seen in normal dermal eccrine ducts (EMA in peripheral cells, CK 10 in intermediate cells, and CK 6, CK 19, and CEA in luminal cells). Nodular hidradenomas exhibited complex patterns suggesting relationship between tumor cells, including clear cells, and normal secretory coil cells (CK 7, CK 8, CK 19, and EMA); however, dermal‐duct and epidermoid differentiation were also detectable. In both cylindromas and spiradenomas, zonal staining patterns were apparent: modified myoepithelial cells were positive for smooth‐muscle‐type actin, while the luminal cells mainly expressed cluctal markers (CK 6 and CK 19) and, less prominently, secretory‐coil markers including CK 7. Eccrine poromas exhibited a widespread reaction for CK5/6 and EMA, analogous to peripheral dermal duct cells, but focal maturation toward inner‐ductal and secretory‐coil cells was also demonstrable. The staining pattern observed in trichoepitheliomas resembled that of the outer but not the inner root sheath. In conclusion, the detailed marker profiles obtained in the present study have broadened our understanding of the differentiation and nature of these highly singular

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01147.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Expression of cell adhesion molecules regulates epithelial cell differentiation and organization of complex tissues such as skin. The CD44 family of adhesion molecules is generated by alternative splicing of up to 10 variant exons encoding inserts into the extracellular domain. Expression of CD44 variant exons has been correlated with metastatic potential of some epithelial malignancies. We studied the distribution of total and variant CD44 isoforms containing exons v4, v6, and v9 in normal skin, basal cell carcinoma, and in control tissues using immunohistologic assays. While normal epidermis and other stratified squamous epithelia reacted strongly with antibodies specific for standard CD44 (CD44S) and CD44 isoforms containing exons v4, v6, and v9, the epithelium of eccrine glands was reactive, often in a polarized distribution, only with antibodies specific for CD44S and isoforms containing exon v9. These studies suggest that differential expression of CD44 variant exons may be important in development and organization of epithelial structures within skin. Malignant cells in basal carcinoma tissues were found to have low reactivity with antibodies specific for CD44S or variant CD44 molecules. The low expression of CD44 molecules in basal cell carcinomas may play a role in the relatively low probability of metastasis of these neoplasms.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01148.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Keratoacanthoma (KA) is generally considered to be a clinically and histologically distinct entity, but it often remains difficult to separate from well‐differentiated squamous cell carcinoma (WDSCC). Recently, trisomy 7 has been identified in squamous cell carcinoma of the skin. In this study, we examined classical KA (n=6), WDSCC (n=7) and squamous cell carcinoma with KA‐like features (SCC‐KA) (n=8) for trisomy 7 by fluorescence in‐situ hybridization (FISH) to determine if this chromosomal abnormality is unique to squamous lesions diagnosed as WDSCC, or shared by both KA and SCC. In addition, the pertinent clinical‐histopathologic findings were summarized. Trisomy 7 was identified in one KA, one SCC‐KA and two WDSCC. This study demonstrates that there is a chromosomal abnormality shared by KA and SCC, providing further evidence that KA is most likely a form of SCC. Further studies are required to determine if trisomy 7 in these lesions is of prognostic s

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01149.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Three cases of nodular‐cystic fat necrosis presenting with focal lipomembranous changes are reported. The lesions consisted of solitary (cases 1 and 3) or multiple (case 2) subcutaneous nodules on the upper (case 1) or lower (cases 2 and 3) extremities which had evolved over weeks to years. At surgical excision, solitary or multiple, freely mobile nodules within a cystic cavity were observed. Histologically, encapsulated fat nodules showing variable amounts of necrosis without marked inflammatory changes were present. Focal lipomembranous changes were observed in some nodules. Our observations seem to support the concept that lipomembranous changes are nonspecific and uncommon patterns of fat necrosis caused by a wide variety of local or systemic events that may cause a compromise in the blood supply of the subcutaneous tissu

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01150.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

An 11‐year‐old Hispanic female was referred for evaluation of multiple asymptomatic papules which had slowly developed since age two. The patient had no significant medical history and no family members, including an older sibling, were similarly affected. Physical examination revealed multiple 2–6 mm flesh‐colored to erythematous papules over the posterior scalp, trunk, and extremities, predominantly in a midline distribution. No lentigines, blue nevi, or evidence of endocrine ab‐normalities were noted. Echocardiogram was normal with no evidence of myxoma. Six cutaneous biopsies showed myxomas, as evidenced by well‐circumscribed dermal tumors composed of stellate, dendritic cells embedded in a mucinous stroma. Special stains and electron microscopy confirmed the H&E findings. Immunoperoxidase staining for Factor XIIIa was positive in the dendritic tumor cells, suggesting a relationship with dermal dendrocytes. This patient represents a case of multiple cutaneous myxomas without other components of Carney's complex of myxomas, spotty pigmentation, endocrine overactivity, and a variety of uncommon tumors. A long‐term follow‐up is indicated since some of the above cited elements of the syndrome could a

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01151.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Brooke‐Spiegler syndrome is characterized by the development of multiple trichoepitheliomas and cylindromas. In addition, multiple spiradenomas have been observed in this autosomal‐dominant inherited disease. We report a 53‐year‐old woman with multiple cylindromas on the head and neck and multiple trichoepitheliomas on the face. Additionally, she had had since birth a plaque on the right side of her neck containing two nodules with features of both cylindroma and trichoepithelioma. Immunohistochemical investigations revealed in the basaloid cells of trichoepithelioma an expression of CK5/6, CK 14, CK 17, CK 19 and vim en tin. The cells of the cylindroma lacked vimentin but expressed additionally CK 7, CK 8 and CK 18. The occurrence of cylindroma and trichoepithelioma in a single nevoid plaque from a patient with Brooke‐Spiegler syndrome implies an alteration in the stem cells of the folliculosebaceous‐apocrine unit and could be characteristic of t

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01152.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY