摘要:

Leiomyosarcoma (LMS) of dermal and subcutaneous tissues is an uncommon neoplasm. In order to analyze the specialized pathologic features of this tumor, we undertook a histological, ultrastructural, and immunohistochemical study of 9 superficial LMS, including 7 dermal lesions and 2 subcutaneous neoplasms. These were compared with 12 examples of “deep” extracutane‐ous LMS. Metastases to the skin from two of the latter neoplasms were also examined. Immunohistochemistry was found to be a useful diagnostic adjunct to light microscopic and ultrastructural studies in that all LMS coexpressed vimentin and des‐min, regardless of site, and 90% also expressed muscle‐specific actin. Variable expression of cathepsin B and niyelin basic protein was noted in 8 and 10 tumors, respectively, whereas none contained cytokeratin. Weak cytoplasmic positivity for epithelial membrane antigen was seen in 1 dermal and 3 extracutaneous LMS. Of 7 dermal LMS, 4 contained S‐100 protein, whereas this determinant was found in only 1 of 12 extracutaneous tumors. Conversely, Leu 7 reactivity was present in 7 of 12 extracutaneous LMS, but only 2 of 9 superficial lesions. Review of clinical features confirmed that subcutaneous LMS is capable of aggressive behaviour, whereas dermal LMS was more likely to behave in an indolent fashion. However, one example of dermal LMS exhibited aggressive local recurrences and distant metastasis, ultimately leading to the death of the patient. Therefore, careful clinical followup is indicated i

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00533.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Scrotal calcinosis is a rare benign disorder considered idiopathic by most recent authors, although an origin from dystrophic calcification of epidermoid cysts has been proposed. In 3 of 4 cases which were otherwise typical of scrotal calcinosis, there was calcification of the contents of small cysts lined by stratified squamous epithelium. These structures were identified as eccrine duct milia because some of them communicated with eccrine ducts and there were ultrastructural features of eccrine duct differentiation in one case. The eccrine nature of the milia was confirmed using the immunoperoxidase technique for the demonstration of carcitioembryonic antigen (CEA) which serves as a marker of eccrine sweat glands. Since a transition could be seen between degenerating calcified milia and typical nodules, it appeared that the calcific deposits of scrotal calcinosis result from the breakdown of such lesions. Staining of deposits unassociated with cyst walls with the technique for the demonstration of CEA supported this conclusion. An advanced stage of evolution of the disease could account for the absence of visible cysts in many cases. We propose the term “hidrocalcinosis of the scrotum” for this distinctive form of cutaneous calcificat

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00534.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Circulating immune complexes (GIG) containingTreponema pallidumproteins have been preliminarily implicated as inducers of a neutrophilic vascular reaction in early human cutaneous lesions of secondary syphilis. To clarify the role of specific CIC in producing cutaneous and renal lesions, 12 rabbits were studied at the following intervals after induction of disseminated syphilis: 20 days (4 rabbits: biopsies of normal and lesional skin for direct immunofluorescence (IMF) for (IgG, IgM, IgA, Clq, C3, C4), fibrin, andT. pallidumproteins; routine histology; and im‐munoblots of serum for CIC containingT. pallidumproteins); 21 days (4 rabbits: as at 20 days without IMF forT. pallidumprotein); 23 days (4 rabbits: as at 20 days without IMF); 30 days (same 12 rabbits restudied with routine histology of normal and lesional skin; kidneys from 4 rabbits removed for routine, IMF, and electron microscopy (EM). Treponemal polypeptide antigen (MW‐87kd) was demonstrated in CIC from rabbits. Routine cutaneous histology showed evolution of lesions from an early neutrophilic vascular reaction to the typical lymphoplasmacytic reaction. IMF showed vessel‐based immunorcactants in 3 of the 4 rabbits tested at 20 days and 1 of 4 at 21 days, andT. pallidumproteins in 3 of 4 rabbits at 20 days. Routine histology, IMF, and EM studies of glomeruli showed glomerular abnormalities, but no evidence of immune deposits containing specificT. pallidumprotein. Skin and kidney studies of 4 controls were all negative. These data indicate a role for specific immune complexes in the pathogenesis of cutaneous lesions in this rabbit

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00535.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Thirty‐nine melanocytic nevi of varying histologic structure and stages of development were examined for the presence of S‐100 protein and melanoma‐associated antigens (MAA) by the indirect immunoperoxidase technique, using a polyclonal anti‐serum to S‐100 protein and monoclonal antibodies to MAA. S‐100 protein and MAA were found in the cells of all types of nevi, including dysplastic and congenital nevi. While S‐100 protein was present in nevus cells at the epidermodermal junction and at all levels of the dermis, the melanoma‐directed monoclonal antibodies BM 24‐2, NKI/C‐3, Mel‐1, and Mel‐2 reacted most prominently with the superficial (A‐type) nevus cells; the B‐ and C‐type cells being negative in these cells. An exception to this rule was melanoma‐monoclonal antibody 34.1 which reacted with nevus cells at all levels; deep C‐cells often being more intensely stained than the subepidermal A‐cells. The cells of the different nevus cell layers, known to differ morphologically and enzymatically, are, thus, also different in their expression of MAA. Our data suggest that for most antibodies these variations may be due to differences in the metabolic activities of nevus cells at v

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00536.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Agminated, or grouped Spitz nevi are a curious expression of nevomelanocytic growth; and represent an uncommon manifestation of these nevi. We encountered an example of agminated Spitz nevi present at birth, which showed progression and rapid growth over months. All showed histologic Spitz nevus features. Avidin‐biotin immunohistochemical techniques using anti S‐100 protein, neuron‐specific enolase (NSE), and melanoma‐specific monoclonal antibody, HMB‐45 labelled the nevi as follows: diffuse S‐100 and NSE staining in most and HMB‐45 positivity in sporadic cells deep in the dermis. HMB‐45 reactivity occurred in both spindle cells and ep

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00537.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The Lafora type of progressive myoclonus epilepsy is a rare and fatal familial disease characterized by seizures, myoclonus, and dementia. This diagnosis was confirmed in 2 patients by demonstrating the presence of intracytoplasmic polygkicosan bodies, or Lafora bodies, in the peripheral portion of the eccrinc sweat gland duct. Exclusive use of the periodic acid‐Schifl stain is recommended for demonstrating these diagnostic inclusions. Electron microscopy reveals fine pale‐staining filaments, line clark‐slaining granules, and dark‐rimmed vacuoles within these non‐inembranc‐bound inclusions. Skin biopsy is the preferred method of confirming the diagnosis of Laf

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00538.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A case of superficially invasive well‐differentiated cutaneous squamous cell carcinoma containing numerous mucin‐proclucing cells both within the epidermal and dermal components is reported. Hislological, histochemical and immunohistochemical overlap exists between these cells and those of extramammary Panel's disease. The presence of mucinous metaplasia within this tumor supports an intraepidermal origin for extramammary Paget's dise

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00539.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Basal cell carcinoma displays a myriad of histopathologic variants, some of which are related to the different lines of differentiation, vis‐a‐vis, squamous, pilar, eccrine, or sebaceous. We herein report an example of a rare signet‐ring, clear‐cell variant. Our diagnosis is primarily based on the histopathologic features of the tumor, namely, the dermal nests of tumor cells with peripheral palisading and locally retracted fibroblastic stroma. Several nests are folliculocentric. The tumor cells are glycogen‐rich, mucin‐negative, pankeratin‐positive, cytokeratin‐negative, S100 protein‐negative, and carcinoembryonic antigen‐negative. Based on the histopathology and the results of the special stains we propose that the signet‐ring clear‐cell variant of basal cell carcinoma is differentiating in the direction of the outer root sheath cells

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00540.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

We report a 24‐year‐old male patient with sarcoidosis who developed subcutaneous nodules on his thighs and buttocks at the sites of repeated intramuscular injections. Histologically, there were extensive areas of caseation necrosis surrounded by typical sarcoidal granuloma. The nodules regressed spontaneously over a period of 4 months, concurrently, both his bilateral hilar adenopathy and the results of immunological tests, returned to normal. We think that these lesions developed at the sites of subcutaneous scar tissue induced by frequent injections in childhood. We believe the pathomechanism to be similar to that for scar sarcoidosis, and that the extensive caseation necrosis reflected a regressing stage of the dise

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00541.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1988.tb00542.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY