摘要:

The skin basement membrane zone is comprised of two major ultrastructural regions, four associated structures, and at least 17 different antigens. In this brief review, the ultrastructure, antigenicity, and ontogeny of normal human skin will be discussed in detail, as will alterations in expression or immune response to selected basement membrane components in specific mechanobullous and autoimmune diseases.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01375.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

The lesions of nine patients with early striae distensae (SD) during puberty were examined by light and electron microscopy. Specific changes were seen in very early stage SD, and in clinically uninvolved skin 0.5 to 3 cm remote from the edge of the long axis of the SD lesions. Sequential changes of elastolysis accompanied by mast cell degranulation appeared first, followed by an influx of activated macrophages that enveloped fragmented elastic fibers. The relationships among elastic fibers, mast cells, and macrophages seen in the present work suggest their critical roles in the process of SD formation, especially in the early stage. Our results also indicate that the elastic fiber is the primary target of the pathological process, and the abnormalities extend as far as 3 cm beyond the lesion into normal skin.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01376.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Electron microscopy has revealed that the deposition of immunoglobulin in the skin of lupus erythematosus (LE) patients occurs on and below the basal lamina of the basement membrane (BM). The composition of the BM is now to some extent known, and antibodies have been developed against several of its individual components. In this study, we attempt to elucidate the status of some matrix molecules in the dermoepidermal junction in LE. Lesional and nonlesional skin from LE patients was examined using immunofluoresence microscopy with monoclonal and polyclonal antibodies against 6 matrix molecules. Immunoelectron microscopy using monoclonal antibodies was used to discern changes in type IV and type VII collagen. By immunofluorescence microscopy, type IV collagen, type VII collagen, and fibronectin were altered in lesional skin. There was a statistically significant correlation between the presence of immunoglobulin and alteration of type IV collagen and type VII collagen in lesional skin. The alterations in type IV and type VII collagens were confirmed on immune‐electron microscopy which showed fragmentation of staining of both antigenic components, particularly type IV collage

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01377.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Traditionally, the pigmented purpuric eruptions (PPE) have been subdivided into four histologic categories, based upon variations in histologic pattern and clinical morphologies. In recent years, it has become apparent that the lesions behave similarly, and this family of eruptions has become grouped under the term PPE. The pathogenesis of these eruptions is largely unknown. In this study, we examined 13 examples of the histologic and clinical subtypes of the PPE with a panel of hematolymphoid immunophenotypic markers, in hopes of better understanding the pathogenesis of these curious eruptions. All lesions examined showed a predominantly T cell infiltrate composed of a mixture of OPD4 (CD4) positive and OPD4 negative cells. B cells were rare. Macrophages did not comprise a significant component of the infiltrate. The staining pattern was relatively constant, independent of the histologic pattern (i.e., Schamberg's vs. Gougerot‐Blum, etc) of the PPE. Our results suggest that PPE are a group of related eruptions which may have a common pathogenesis. It seems likely that the vascular damage and erythrocyte extravasation are secondary to a localized cell‐mediated immunologic ev

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01378.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

The scalp is frequently involved in the autoimmune skin disease pemphigus. This study demonstrates the distribution of pemphigus antigen in the scalp terminal hair follicle; as well as being found in the epidermis, it is distributed throughout the whole hair follicle outer root sheath and in the dermal bulb matrix cells. The increase in volume of target antigen offered by the follicular epithelium could be a factor determining scalp involvement in pemphigus.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01379.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Three malignant melanomas, two melanoma metastases, two junctional dysplastic nevi, and normal skin were embedded in Lowicryl. Ultrathin sections were incubated with HMB‐45 and a gold‐labeled anti‐mouse antibody. Gold particles indicating the presence of HMB‐45 were found in melanosomes Stage 1 and 2 and in the non‐melanized portion of melanosomes Stage 3. Melanosomes Stage 4 and melanosome complexes in keratino‐cytes, as well as in melanophages, were consistently negative. No specific labelling with HMB‐45 was seen in eccrine glands o

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01380.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Heavily‐pigmented melanocytic neoplasms are difficult to evaluate on routine hematoxylin and eosin stained slides because pigmented melanocytes arc difficult to distinguish from the numerous melanophages that are usually seen in the background of these lesions. Immunoperoxidase staining for SI00 protein or HMB‐45 antibody using diaminobenzidine (DAB) as chromogen, which forms a brown product, does not adequately distinguish melanocytes from melanophages. We modified this technique by replacing hematoxylin as the counterstain with azure B, which stains melanin green‐blue. Thus, positive melanocytes appear brown while melanin granules in their cytoplasm are green‐blue. However, negative melanophages only stain green‐blue. This technique is useful in evaluating heavily pigmented melanocytic lesions such as malignant melanomas, melanosis of regressing malignant melanoma, residual malignant melanoma in areas of granulation tissue with melanophages, blue nevi, pigmented spindle cell variant of Spitz's nevi an

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01381.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Image analysis of DNA content was performed from single nuclei of melanoma monolayer imprints made from fresh frozen (issue of 14 patients with primary malignant melanoma and 16 patients with local recurrences at the incision site and local or distant metastases. This procedure requires fewer cells and is an advantage when the quantity of tumor available is limited, especially in thin low Breslow depth cutaneous melanomas. Image analysis allowed reproducible measurement of DNA ploidy from 100 cells. The frequency of aneuploidy was similar in primary and metastatic melanomas. Three of 3 patients with euploid primary melanomas showed no evidence of recurrences or meta‐stases, though one died of unrelated disease with short follow‐up. The 4 patients with primary melanoma who developed metastases had aneuploid primaries; two of these patients died of metastatic disease. Three of 4 patients with euploid metastatic tumors were free of disease at last follow‐up, and 1 patient died with stable disease. Nine of 12 patients with aneuploid tumors died of metastatic disease. The frequency of DNA ploidy in the present image analysis study correlated with previous (low cytometry studies. In 9 patients with primary tumors with a Bin‐slow depth greater than 0.75 mm, the. DNA content was also determined in nuclei obtained from formalin‐fixed paraffin‐embedded tissue. The frequency of aneuploidy was higher in fresh tissue (7 of 9) as compared with paraffin‐embedded tissue of the same c

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01382.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Sections from paraffin‐embedded tissues of lesions thought to represent verrucous lesions (benign verrucous acanthomas: BVA) as well as materials from controls including seborrheic keratoses (SK), irritated SK, and verruca vulgaris from areas other than the genitalia were analyzed for the presence of multiple human papillomaviruses (HPV) including types 6, 11, 16, 18, and 33 using DNA amplification methodology. HPV DNA sequences were not found in amplified samples of BVA, SK, and irritated SK samples, but were identified in those obtained from verruca vulgaris. Our results suggest that BVA, SK, and irritated SK are generally not associated with HPV DNA even though certain of these lesions may histologically mimic the architecture of verruc

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01383.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Acral erythema (AE) and eccrine squamous syringometaplasia (ESS) are distinctive clinico‐pathological entities described in patients receiving chemotherapy for malignancies, mainly myelogenous leukemia. In no case of chemotherapy‐induced AE have histopathologic changes of ESS been described. We report a patient with acute myelogenous leukemia who had typical AE due to cytarabine with histopathologic ESS. The latter findings in a case of AE may be a useful diagnostic clue for distinguishing chemotherapy‐induced AE from palmo‐plantar erythema of graft‐vs‐ho

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1991.tb01384.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY