ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00260.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

A bright, continuous, granular deposition of immunoreactants at the dermo‐epidermal junction (DEJ) of lesional skin is highly suggestive of cutaneous lupus erythematosus (LE). A recent study of the direct immunofluorescence (IF) of sun‐exposed skin in normal adults has demonstrated findings similar to the bright, continuous granular pattern found in cutaneous LE. This data suggests that positive IF from sun‐exposed cutaneous lupus lesions is nonspecific. Forty‐one healthy adults, without a history of dermatoses or photosensitivity, presenting to the dermatology clinic for the excision of skin cancers were studied. Excess non‐lesional tissue, removed from Moh's excision sites (sun‐exposed face and neck) in order to obtain appropriate cosmetic closure, was examined for the deposition of immunoreactants. The specimens were incubated with iluoresceinated monovalent anti‐human immunoglobulin specific for IgG, IgA, IgM, C3, Clq, and fibrinogen and examined independently by 2 immunodennatologists without prior knowledge of patient or site. None of the samples demonstrated immunoreactant deposition consistent with cutaneous LE. IF of several specimens (21/41) had a weak(1+ or 2+), interrupted pattern of fibrinogen at the DEJ, – a common, non‐specific finding. Weak, interrupted, linear and granular patterns were seen with IgM (10/41), Clq (9/41), IgG (2/41), IgA (2/41), and C3 (1/41). Fibrinogen was the only immunoreactant demonstrating a bright (3+), continuous, granular pattern (4/41). This data suggests that sun‐exposure alone does not induce the deposition of immunoreactants at the DEJ in a pattern similar to that fo

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00261.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

In evaluating histologically malignant infiltrates in the skin, it is often challenging to distinguish granulocytic sarcoma (GS) from selected cases of peripheral T‐cell lymphoma (PTCL). These lesions have clinical features in common, in addition to shared histologic attributes. These include similarity in dermal distribution and growth pattern, nuclear characteristics, propensity to recruit other inflammatory cell types, and production of matrical sclerosis. In order to determine if immunohistology could contribute to differential diagnosis in this setting, we analyzed 15 cases of mucocutaneous GS, and compared them with 11 cases of well‐documented PTCL. Antibodies in the CD15, CD20, CD34, CD43, GD45, CD45RO, and CD68 groups were used, as well as antimyeloperoxidase (anti‐MPX), anti‐lysozyme (anti‐LYSO), Mac387, and MB2. Anti‐LYSO and anti‐MPX were sensitive and specific markers of GS, labeling 93% and 80% of GS cases, respectively, and no cases of PTCL. Anti‐CD 15 and MB2 were also specific for GS, but each labeled only (50% of GS cases. CD34, CD68, and Mac 387 were specific but insensitive markers of GS. CD43 and CD45 were not particularly useful discriminants, with each being seen in 93% of GS cases, but also 64% and 100% of cases of PTCL, respectively. CD45RO was specific for PTCL; it was present in 82% of PTCL cases and no GS cases. Thus, conjoint reactivity for CD43, CD45, MPX, and LYSO characterizes GS, and differs from the pattern of PTCL, which is characterized by reactivity for CD45 and CD45RO, occasional reactivity for CD43, and lack of other sp

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00262.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Reported are oral mucosal warts (HPV common antigen‐positive) from 7 adult HIV+patients in which there was cytologic atypia and disordered growth. Lesions were papillary, white to red in color, and were located on the lip, gingiya, palate, tongue, and buccal mucosa. Histologically, the keratinocytes in the lesions exhibited atypical features in the form of hyper‐chromatism and karyomegaly. Koilocytes were frequently seen in the upper level keratinocytes where HPV common antigen was identified. The dysplastic areas, which ranged from mild to severe, typically showed abrupt limiting margins. All lesions exhibited intense PCNA reactivity from basement membrane to surface. Nuclei of mid‐level and basal keratinocytes of 3 specimens stained positively for p53 protein. We believe that the atypia found in these lesions represents cytologic change that has malignant potential. The subtype of the HPV in these lesions has not yet been deter

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00263.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

The human hematopoietic progenitor cell antigen (CD34) is a cell surface protein expressed by human hematopoietic progenitor cells, vascular endothelium, and many mesenchymal tumors. Sections from six samples of normal skin and from 41 epithelial tumors of the skin were studied. Immunostaining of epithelial cells from the external root sheath below the attachment of the arrector pili muscle and above the matrix cells was noted in normal samples. Tumors derived from or differentiated toward cells of the outer sheath, especially trichilemmomas, were immunostained with QBEND/10 (anti‐CD34 antibody), whereas other epithelial tumors studied were negative. CD34 could serve as a marker of outer sheath cell derivation and may well be of value in the distinction between trichilemmomas and other lesions with similar histopathological feature

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00264.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

CD44 is a widely distributed cell surface protein thought to be involved in multiple steps of normal immune cell function, including T‐cell activation, and in cellular adhesion where it mediates cell attachment to hyaluronate. In normal skin, CD44 is found by immunohistochemical means to be primarily in eccrine coil cells. In this study, we have looked at the expression of CD44 in normal scalp and in two different hair disorders, androgenetic alopecia and alopecia areata. In normal scalp and androgenetic alopecia, CD44 was found in its normal distribution in eccrine coil cells. In scalp of 30 patients with alopecia areata, there was no expression of this glycoprotein. Patients were also assessed pre and post treatment for their alopecia areata, and even though they had no significant hair regrowth, 2 patients regained expression of CD44, indicating a variable expression of this protein in the alopecia areata disease process. The absence of CD44 expression in alopecia areata‐affected scalp may give further information regarding the pathogenesis of this dise

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00265.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Mid‐dermal elastolysis is a well‐defined clinical and histopathologic entity manifested by fine wrinkling of the skin and a mid‐dermal loss of elastic fibers. Ulfrastructural and hislologic studies were performed in an attempt to better define the cause of the elastolytic process. Biopsy specimens from the lesions of 3 patients with mid‐dermal elasiolysis were studied at light and electron microscopic levels. Ultrastructural evidence of normal elastic fiber engulfment by activated macrophages was observed; however, some fields also demonstrated envelopment of abnormally degenerated elastic tissue. Although there are many potential causes of this degeneration, photodistribution of the lesions suggests that ultraviolet damage is a primary inciting

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00266.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

We attempted to evaluate whether Widéhn's quick re‐embedding method from paraffin blocks is useful for dermatopathological diagnosis. Regarding fine preservation of nuclei, tonofibrils and desmosomes of keratinocytes in normal skin, we could recognize no difference between the quick re‐embedding method and traditional re‐embedding methods. However, preservation of mitochondria and Birbeck granules in Langerhans cells was poor regardless of the method used. Furthermore, we studied the ultrastructural features of some viral skin diseases including mollscum contagiosnm, herpes simplex, varicella‐zoster and verruca vulgaris, and some skin tumors, including angioleiomyoma, angiosarcoma, Merkel cell carcinoma and amelanotic melanoma using the quick re‐embedding method. We determined that all viral structures were sufficiently preserved by the quick method to observe the virions and developement of the virus. Myolilaments and dense bodies of angioleiomyoma, Wcibel‐Palacle bodies of angiosarcoma, neurosecretory granules of Merkel cell carcinoma and melanosomes of amelanotic melanoma were recognized by the quick method. From these results, we concluded that the quick re‐embedding method is useful for the diagnosis of skin diseases, especially viral skin diseases and so

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00267.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

To characterize the potential role of mast cells (MC) in angiogenesis, this study tests the hypothesis that MC may be more abundant in angiolipomas than in classic lipomas. MC counts were compared in 13 subcutaneous angiolipomas and 15 subcutaneous classic lipomas stained with Giemsa. Angiolipomas had ten times as many MC as did classic lipomas (25.34 ± 2.83 versus 2.41 ± 0.37 per mm2, mean ±SE). To clarify whether this difference was primary (angiogenic activity) or secondary to the increased vascularity, MC were counted in 8 longstanding cutaneous capillary hemangiomas versus 13 cutaneous capillary hemangiomas of recent onset (pyogenic granulomas). If MC were mediating primary angiogenesis, one would expect them to be present in greater numbers in early than in late hemangiomas. To the contrary, however, long‐standing hemangiomas were found to have significantly more MC than had those of recent onset (52.48 ± 14.99 versus 6.59 ± 3.37 per mn2, mean ±SE). These results suggest that MC may not play an essential, early role in the proliferation of blood vessels in angiolipomas and hemangiomas, but rather may be related to maturation of blood vessels in these

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00268.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

We report 2 women, aged 83 and 63 years, who presented with multiple asymptomatic, slowly progressive, coalescing, skin‐colored papules affecting the sides of the neck and lower abdomen. Incisional biopsies obtained from both patients revealed elastolysis in the papillary dermis. These 2 cases represent pseudoxanthoma elasticum‐like papillary dermal elastolysis, a recently described entity, the etiology of which remains uncl

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00269.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY