ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00243.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Recently, T‐cell lymphoma localized to the subcutaneous tissue has been reported. We report the clinical, histolpgic, immunohistochemical, and molecular genetic findings in 6 patients who we believe had this peculiar T‐cell lymphoma with its unique morphologic and clinical features. All patients presented with deep‐seated nodules, most frequently on the extremities, and with systemic complaints of low‐grade fever, fatigue, myalgias, and weight loss. In all cases, the neoplastic lymphocytic infiltrate was confined to the subcutaneous tissue, predominantly in a lobular pattern. Hemorrhage, necrosis, and rare erythrophagocytosis were also seen. Immunohistochemical staining was predominantly T‐cell reactive (CD43, CD3, and CD45RO). Clonal rearrangements of the β and γ chains of the T‐cell antigen receptor genes were found in 1 case. Three of the 6 patients died within 22 months of the diagnosis of lymphoma. We believe that subcutaneous T‐cell lymphomas are a distinctive group of peripheral T‐cell lymphomas with unusual clinical and morphologic features and that they should be distinguished from other

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00244.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Epidermolysis bullosa simplex (EBS) is an uncommon genetic skin disease characterized by fragility of the basal keratinocytes and propensity to develop blisters. While a panel of antibodies against type IV collagen, laminin, and bullous pemphigoid antigen has been used to identify cases of EBS in frozen sections, we have found that a monoclonal antibody detecting cytokeratin in basal keratinocytes is useful in paraffin sections. Formalin‐fixed, paraffin‐embedded tissues from 12 patients with EBS were studied with the following monoclonal antibodies: MNF11G (DAKO, Carpintería, CA), CAM 5.2 (Becton Dickinson, San Jose, CA), and AE1‐AE3 mixture (Boehringer Mannheim Corp, Indianapolis, IN). Histologically, all the cases had focal vacuolization of the basal cell layer, with areas of dermal‐epidermal separation. MNF116 was strongly positive in the basal keratinocytes, including the vacuolated ones, and demonstrated the presence of fragments of keratinocytes attached to the floor of the blister. CAM 5.2 stained sweat ducts only. AE1‐AE3 was weakly positive in the basal cells, and almost completely negative on the fragmented basal cell keratinocytes. We consider that the immunostain with MNE116 in tissues fixed routinely in formalin and embedded in paraffin is helpful for the direct demonstration of the level of splitt

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00245.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Lichen planus is an inflammatory dermatosis which is characterized histologically by an intense lymphocytic infiltrate at the dermal epidermal junction. This frequently results in disruption of the basement membrane zone, occasionally causing clinical blisters. In order to better understand the specific portion of the basement membrane zone which is disrupted by the lymphocytic infiltrate, we examined 7 cases of lichen planus with antibodies directed against anchoring filaments (GB3), the bullous pemphigoid antigen, anchoring fibrils (type VII collagen) and type IV collagen. In lesions without separation at the BMZ, all antibodies were strongly expressed, as in normal skin. In lesions with early separation, there was a focal decrease in GB3 staining, but types VII and IV collagen labelled normally. In lesions resulting in blisters, GB3 staining was essentially absent, and anti‐types IV and VII collagen remained, but stained in a disrupted, less discrete pattern. The bullous pemphigoid antigen showed only slight deviation from the normal staining pattern. These findings suggest that the basement membrane zone in lichen planus is disrupted in the lamina lucida region. The lamina densa and sub‐lamina clensa zones remain intact even in bullous lesions of lichen pla

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00246.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Thin sections of dermatofibromas, of a basal cell epithelioma, a patch stage lesion of Kaposi's sarcoma, a case of angiolymphoid hyperplasia with eosinophilia, and one case of malignant mastocytosis were incubated with antibody to factor XIIIa (FXIIIa). Regardless whether the tissue had been embedded in Lowicryl K4M, Epon, or Araldite, labeling was found in dermal dendrocytes, mast cells, and endothelial cells. In dermal dendrocytes, the reaction product was seen in dilated cisternae of the rough endoplasmic reticulum as well as free within the cytoplasm. In endothelial cells, FXIIIa was localized within Weibel Palade bodies, free within the cytoplasm, and in villous projections into the vasucular lumen. In mast cells, the reaction was found in mast cell granules exclusively. Mast cells, dermal dendrocytes, and endothelial cells have in common that all three express FXIIIa, belong to the microvascular unit, and are increased in number during angiogenesis and in fibrovascular processes. It thus seems that these cells are functionally related.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00247.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

The monocyte/histiocytic response in granuloma annulare has not been extensively studied. We studied the immunohistochemical staining pattern in granuloma annulare by using a panel of markers for “histiocytic” cells including Ham 56, KP1, factor XIIIa, Mac 387, vimentin, and lysozyme. The infiltrate failed to stain with the histiocytic markers, except with antibodies against vimentin and lysozyme. Commonly used histiocytic markers for infectious, immunogenic, and foreign body granulomas fail to stain the infiltrate in granuloma annulare. We hypothesize that a locally derived, immunologically distinct population of histiocytes produces the reaction pattern of granuloma annul

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00248.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

The coexpression of cytokeratin and vimentin intermediate filaments has been immunohistochemically evaluated in 124 benign and malignant sweat gland tumors of various types in comparison to normal sweat glands. In addition, all neoplasms have been stained by an antibody to alpha‐smooth muscle actin. Epithelial cells reacted with the pan‐cytokeratin antibody lu‐5. In normal sweat glands, vimentin immunoreactivity was restricted to myoepithelial cells and to some cells of the coiled duct. In benign sweat gland tumors (n=88), coexpression of vimentin and alpha‐smooth muscle actin was frequently found in basal cells of neoplasms considered to differentiate towards the secretory coil of the eccrine or apocrine gland. These included eccrine spiradenoma, apocrine cystadenoma, hidradenoma papilliferum, syringocystadenoma papilliferum, and cylindroma. Thus, in these tumors, vimentin‐reactive cells corresponded to myoepithelial cells. Vimentin‐positive cells were also found in 14 of 36 sweat gland carcinomas, including 1 case of sclerosing sweat duct carcinoma, 1 case of porocarcinoma, 4 cases of eccrine adenocarcinoma, 1 case of mucinous eccrine carcinoma, and 5 cases of apocrine adenocarcinoma. Co‐expression of vimentin and alpha‐smooth muscle actin was observed in some cells of eccrine and apocrine adenocarcino‐mas. Therefore, in these neoplasms, some vimentin‐positive cells appear to represent myoepithelial cells. In contrast, vimentin‐positive cells in all other malignant tumors did not express alpha‐smooth muscle actin. Our results indicate that coexpression of cytokeratin and vimentin may be frequently found in a variety of benign and malignant sweat gland tumors. In the majority of these neoplasms, vimentin‐positive cells correspond to myoepithelial cells. Because vimentin is not specific for myoepithelial cells, additional stains for alpha‐smooth muscle actin should be performed to prove the myoepithelial nature

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00249.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

In prior studies, we showed that most CD8+cells infiltrating skin lesions of CD3+CD4+mycosis fungoides were CD3+T‐line‐age tumor‐infiltrating lymphocytes (TIL) whose overall phenotype was suggestive of MHC‐restricted cytotoxic T lymphocytes (CTL). However, their lack of cytotoxic‐associated granzyme A mRNA suggested that they might be inactivated CTL precursors. In this study, we used single‐ and double‐label immunohistologic techniques to assess the expression of TlA‐1‐reactive protein and HLA‐DR by these CD8+TIL. Monoclonal antibody TIA‐1 recognises a novel family of proteins expressed preferentially by cytotoxic cells, including some that lack grauzyme A. HLA‐DR is a marker of T‐cell activation. Single‐label studies of 32 cases showed that CD8+TIL and TIA‐1+cells constituted a variable minority of the total cellular infiltrate and had a similar distribution. Double‐label studies of 14 cases showed that in most instances the aggregate phenotype of the majority of CD8+TIL was CD3+TIA‐1+HLA‐DR+CD56−CD57−. These findings suggest that many of the CD8+TIL within skin lesions of CD3+CD4+mycosis fung

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00250.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

An unusual case of primary adenocarcinoma of the vulva is described. It combined features of the three different types of adenocarcinoma of the skin of the vulva which are currently recognized, i.e. sweat gland carcinoma, adenocarcinoma derived from supernumerary mammary glands, and extramammary Pagel's disease (EMPD). Central in this tumor was a recently recognized type of cutaneous gland which appeared special for the anogenital region and was distinguished because it combined morphological features of eccrine, apocrine and mammary glands. As it most resembles mammary glands, it is named “mammary‐like gland”. On the basis of the case presented and of a critical review of the literature, it was concluded that, with the exception of a few sweat gland carcinomas similar to those elsewhere in the skin, adenocarcinomas of the skin of the vulva form a single category of neoplasms with a variable expression of features reminiscent of eccrine, apocrine and mammary gland carcinomas. The data strongly suggested a common derivation from the mammary‐like gland or, in cases of EMPD, its related germinative cells in the ep

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00251.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

We report a 6‐year‐old female with recessive dystrophic epidermolysis bullosa (RDEB) who presented with a very large acquired melanocytic lesion. The lesion demonstrated many features both clinically and histologically that made the distinction from malignant melanoma difficult. The pathogenesis of this lesion and other unusual melanocytic lesions seen in the setting of acute and chronic blistering disorders seems related to repeated episodes of disruption of the dermal‐epidermal jun

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1994.tb00252.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY