ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01299.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01300.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Cutaneous T‐cell lymphomas (CTCL) are a heterogeneous group of lymphoproliferative disorders. We investigated the variable region (Vβ) of the T‐cell receptor (TCR) repertoire in CTCL and compared it to the Vβ repertoire in normal and eczematous skin.We used a panel of 21 anti‐Vβ antibodies and investigated 84 biopsies of 71 CTCL patients (4 parapsoriasis en grandes plaques (PA), 1 lymphomatoid papulosis, 29 mycosis fungoides (MF), 13 Sezary syndrome (SS), 1 CD8+ CTCL, 11 pleomorphic CTCL (PLEO), 12 CTCL not classified). Six biopsies of normal skin and 6 of eczematous skin lesions served as controls. We determined the frequency of the Vβ in normal and inflamed skin and compared it to the percentage of the respective Vβ in the malignant clone of the CTCL patients.The percentage of the Vβ positive CD4+ cells in relation to the total number of T cells in normal skin and inflamed skin differed from the distribution of the Vβ families in the peripheral blood monoiiuclear cells (PBMC). Out of 71 CTCL cases, the clone was identified in 23 (32%). We identified the following clones : 1 V03.1 (1 MF), 7 V05.1 (1 CD8+ CTCL.l CTCL not classified, 1 MF, 1 PA, 3 SS), 1 V|36.7 (1 SS), 7 Vβ8.1/8.2 (2 CTCL not classified,! PLEO, 2 MF, 2 SS), 1 V012.1 (1 PLEO), 3 Vβl7.1 (2 CTCL not classified, 1 MF), 2 Vβ22.1 (1 CTCL not classified, 1 MF), 1 TCR8 (SS). The frequency of the malignant clone Vβ usage corresponded well to the repertoire of Vβ in eczematous skin but not to the repertoire in PBMC. In 6 patients, the malignant clone was mainly localized in the epidermis. In 17 cases, the clone‐specific cells were distributed in epidermis and dermis equally. A retrospective analysis showed that preferential epidermal homing of the clone was associated with a non‐aggressive clinical course.The Vβ usage of CTCL and eczema suggests a special cutaneous microenvironment which might be co‐created by certain (bacterial?) superantigens. A preferential epidermal homing of the clone might have

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01301.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Immunohistochemical studies can augment the clinicopathologic diagnosis of cutaneous T‐cell lymphoma (CTCL). Our goal was to determine whether a panel of 11 T‐cell receptor (TCR) beta chain variable region (Vβ) monoclonal antibodies (moAbs) could consistently identify clonal T‐cell populations within CTCL skin infiltrates, and whether these cells exhibited aberrant T‐cell antigen expression. Biopsies from 24 CTCL and 3 parapsoriasis patients were analyzed. Of the 27 patients, 4 (15%) demonstrated T‐cell clonality by restricted TCR‐Vp moAb staining. The Vp+ restricted cells expressed aberrant antigen profiles. Overall, aberrant antigen profiles were detected in 18/24 (75%) CTCL patients. Vβ18 moAb crossreacted with a 85 kD protein produced by basal and suprabasal keratinocytes. We conclude: 1) Restricted TCR‐Vp expression correlated with aberrant T‐cell antigen profiles; 2) In the absence of a complete panel of TCR‐Vp moAbs, localization of aberrant T‐cell antigen expression can be useful in identifying malignant T‐cells within CTCL skin infiltrates; 3) The detection sensitivity and specificity of the currently available TCR‐VβmoAbs may limit their utility to consistently detect clonal T‐cell populations in CTCL skin biopsies; 4) A 85 kD protein present on basal and suprabasal keratinocytes is recognized by Vβ18 moAb and may be related to immune

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01302.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Adhesion molecules such as integrins and cadherins are thought to play a critical role in T‐cell migration and localization within the epidermis (epidermotropism). The purpose of this study was to correlate T‐cell expression of the integrin GD103 and E‐cadherin in cutaneous T‐cell lymphoma (CTCL). Serial sections of skin biopsies from 22 patients with CTCL and 13 with benign reactive dermatitis were stained with antibodies to CD4, CD103, and E‐cadherin by the avidin‐biotin peroxidase technique. CD 103 was expressed on single epidermotropic CD4+ T‐cells in 9/9 early stage (patch/plaque) CTCL and 6/10 reactive dermatitis biopsies. Less than 30% of dermal T‐cells expressed CD103. All 4/4 late stage (tumor) CTCL were GD103–. Epidermal aggregates of CD4+T‐cells (Pautrier's microabscesses) were CD103–. E‐cadherin was expressed on epidermal keratinocytes and follicular and sweat gland epithelia but not on T‐cells.We conclude that CD 103 expression on cutaneous T‐cells parallels the degree of epidermotropism exhibited in both neoplastic and inflammatory disorders of the skin. E‐cadherin is not expressed on T‐cells infiltrating the skin. Further investigation is necessary to further elucidate the interaction between CD103 and E‐cadherin in facilitating traffick

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01303.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

MT2/CD45RA and anti‐bcl‐2 protein (Bcl‐2) monoclonal antibodies are useful markers in distinguishing follicular lymphomas from reactive follicular hyperplasia of the lymph nodes. We examined biopsy specimens from 11 patients with primary cutaneous B‐cell follicle center lymphomas, 10 patients with cutaneous pseudolymphomas with germinal centers, and 6 patients with inflammatory infiltrates with germinal centers in non‐lymphoid cutaneous tumors (3 basal cell carcinomas, 2 malignant melanomas, and 1 solar keratosis), in order to evaluate the utility of MT2 and anti‐Bcl‐2 antibodies in differentiating benign from malignant germinal center cell proliferations in the skin. Immunohistochemical evaluation of MT2 and Bcl‐2 was focused exclusively on the reactivity of germinal center cells. Specific membranous MT2 positivity was found in 2/11 cutaneous follicle center lymphomas; a diffuse, non‐specific staining pattern was identified in 3/11 follicle center lymphomas and in 1/6 inflammatory infiltrates in non‐lymphoid tumors. A negative MT2 reaction was observed in 6/11 follicle center cell lymphomas, in all cases of pseudolymphomas and in 5/6 inflammatory infiltrates in non‐lymphoid tumors. Bcl‐2 positivity was detected only in 1/11 follicle center lymphomas. Germinal center cells in all other cases were Bcl‐2 negative. Our results suggest that MT2 and anti‐Bcl‐2 antibodies are only of limited value in differentiating primary cutaneous follicle center lymphomas from cutaneous pseudoly

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01304.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Cutaneous lesions of anaplastic large cell (CD30+) lymphoma (ALCL) typically present as solitary or multiple ulcerated nodules. This tumor is histologically characterized by a diffuse dermal and sometimes subcutaneous infiltrate composed of bizarre, pleomorphic, neoplastic cells that may be occasionally multinucleated. We report a case of extranodal spread of ALCL to the skin in a 21/2‐year‐old boy presenting as a widespread papular eruption that on biopsy showed lymphoma restricted to the perivascular and periadnexal der

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01305.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

After intradermal testing with goldsodiumthiomalate (GSTM), 5 out of 8 patients developed skin papules at the test sites, which persisted up to 20 months. The lesions were surgically excised. Histology revealed pseudolymphoma of B and T cell type containing follicular structures and occasional small granulomas. The amount of histiocytic cells among B and T lymphocytes was pronounced, including acid cysteine proteinase inhibitor (ACPI) positive follicular dendritic cells, CD68 positive macrophages, factor XUIa positive dermal dendrocytes and S‐100 positive cells. By electron microscopy, the macrophages contained endosomes loaded with crystalloid material which contained gold in X‐ray microanalysis. Atomic absorption spectrophotometry also confirmed the presence of gold in one specimen. Thus GSTM seemed to accumulate in tissue macrophages leading to constant immunologic activation with lymphoid proliferation and histiocytic respo

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01306.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Nuclear morphometry, immunohistochemical staining with Ki‐67 antibody and mitotic index were studied in primary cutaneous malignant melanomas. The number of Ki‐67 positive cells/200 tumor cells did not correlate with any nuclear morphometrical parameters, and it only approached but did not reach significant correlation with melanoma thickness according to Breslow. The nuclear area, short axis and long axis correlated with melanoma thickness, but the nuclear axis ratio (which reflects the sphericity of nuclei) and melanoma thickness did not show significant correlation. Mitotic index was higher in thick melanomas and in melanomas with high Ki‐67 positivity, large nuclear area, long nuclear short axis, and small nuclear axis ratio. In Cox's stepwise proportional hazard model, melanoma thickness and the nuclear axis ratio were significant independent prognostic factors for patient survival, while the nuclear area, short axis and long axis, gender, age, Clark level, mitotic index and Ki‐67 positivity lacked significant independent prognostic value.The results suggest that the proliferative activity of tumor cells does not alone explain the great importance of tumor thickness as prognosticator in melanoma. The thickness of melanoma measured according to Breslow and the nuclear axis ratio are more efficient prognosticators in melanoma than parameters associated with prolif

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01307.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Whilst tumour thickness is of great value in predicting prognosis for groups of patients in different categories, it is of less value for individual patients. It has been proposed that expression of the nm23 gene, a putative tumour suppressor gene, is associated with improved outcome in a number of human neoplasms including malignant melanoma. We assessed nm23 expression in 22 patients who had primary melanomas>3mm thick. Ten have survived to date and 12 died of melanoma. Using immunohistochemical methods, we found no significant differences in gene expression between the two groups.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1996.tb01308.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY