ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01391.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Cutaneous cysts from a patient with Gardner's syndrome were histopathologically studied in detail. The cysts were, by and large, indistinguishable from ordinary epidermal cysts. However, several distinctive features were found: 1) epidermal or trichilemmal keratinization, 2) mature sebaceous glands connected with the cyst wall, 3) hair matrix‐like structures associated with dermal papilla cells, 4) pilomatricoma‐like changes, 5) intraluminal masses or pericystic deposits of shadow cells variably accompanied with foreign body reaction, 6) foreign body reaction or masses of shadow cells lining completely eroded cysts, 7) the presumptive bulge area, and 8) epithelial islands adjacent to the cyst. Each cutaneous cyst showed a variable combination of the findings described above. Foci of the basal layer of some cyst walls or epithelial islands were immunohistochemically stained with CK19, where CK20‐reactive Merkel cells were also present. These Findings were consistent with those of the bulge area. Unexpectedly, desmin‐reactive muscle bundles, presumably indicating arrector pili muscle, were observed along the cyst wall. Our observations suggest that Gardner's cysts may be derived from putative follicular stem cells which reside in the bul

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01392.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Thin melanomas, measuring 0.76 mm or less, are generally associated with an excellent prognosis. However, a certain subset, of these seemingly innocuous lesions have been reported to develop recurrences. Therefore, the predictive values of currently accepted prognostic indicators have been questioned in thin melanomas. Several studies concerning tumor vascularity in melanoma and certain non‐melanocytic malignancies suggest that the degree of vascularization correlates with growth rate and biologic aggressiveness. In the present study, we determined the vascularity of a small group of Clark level II melanomas that resulted in recurrence, and compared these results to au equal number of nonrecurrent lesions with similar prognostic indicators. Blood vessels were labeled by immunoperoxidase staining techniques for Type IV collagen, and quantified by image analysis. No statistical difference was found between the two groups when mean blood vessel counts and percent vascular area were measured. The recurrent tumors had a mean PVA of 4.68 compared to 4.34 for the nonrecurrent group (p = 0.677). The mean blood vessel count beneath the recurrent group was 29.6 per 400 × field, and the corresponding value for the nonrecurrent group was 31.8 (p = 0.681). Our data is preliminary within this limited group of tumors, yet it suggests that tumor vascularity is not a distinctive prognostic indicator by which eventual outcome can be predicted in thin Clark level II malignant melanom

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01393.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Numerous attempts have been made to apply image analysis in dermatopathology. The technics used comprise measurement of nuclear size, shape, chromatin content, and texture, evaluation of immunohistological slides, assessment of proliferation, pattern analysis, and tumor volume estimation. For commonly accepted routine use, however, image analysis research has to be extended to large numbers of cases, using straightforward and reproducible measuring procedures, and to the development of ready‐to‐use equipment for specific tasks. In this way, image analysis in dermatopathology might supply useful diagnostic tools in addition to conventional microscopy, and may increase our understanding of morphology as a wh

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01394.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Small cell (nevoid) melanomas may provide difficulties in diagnosis as their constituent cell type resembles a benign nevoid melanocyte. In the present study, 10 small cell melanomas were analyzed for the silver staining of their nucleolar organizing regions (AgNORs), and their nuclear area and perimeter were measured by computerized digital image analysis and compared with 10 superficial spreading melanomas lacking small cell differentiation and 10 dermal nevi. The average number of AgNORs per nucleus was 5.83 (SD ± 1.69) for small cell melanomas and was significantly different when compared with 8.49 (SD ± 1.58) for superficial spreading melanomas (p<0.05) and 2.71 (SD ± 0.50) for dermal nevi (p0.05), but both group were significantly different from superficial spreading melanomas lacking a small cell morphology (p<0.05). Counting AgNOR numbers may be useful in evaluating small cell (nevoid) melanomas and provides a technique for differentiating their constituent cell from ordinary nevus cells. Nuclear morphometry determined by digital image analysis may help better define the nuclear size in small cell melanom

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01395.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The histogenesis of cutaneous nerve sheath myxoma (NSM)/ cellular neurothekeoma (CNT) is still controversial. In this study, we examined the ultrastructural features of 16 NSM (3 classical, 11 CNT, and 2 mixed NSM/CNT). We classified the cells into 4 groups ultrastructurally. Type 1 cells were un‐differentiated polygonal cells with ovoid nuclei, cytoplasmic microfilaments, and occasionally with microfilament‐associated dense bodies. In most cells, the cytoplasmic membrane showed focal membranous densities and occasional basal‐lamina‐like material. This cell type comprised approximately 90% of CNT. Type II cells were more differentiated, had ovoid or spindled shapes, were rich in intracytoplasmic filaments, and were surrounded by continous basal lamina. These cells were consistent with Schwann cells and were present in the classical and mixed forms of NSM, and in a single case of CNT. Type III cells had features of perineurial cells and were relatively rare in classical NSM. Type IV cells resembled fibroblasts and were encountered in all variants of NSM. These results support the‐view that 1) the classical NSM has neural (mainly Schwann cell) differentiation, 2) CNT is predominantly composed of undifferentiated cells with partial features of Schwann cells, smooth muscle cells, myofibroblasts and fibroblasts, suggesting a divergent differentiation, and 3) CNT and NSM represent a histologic spectrum, but in CNT, the neural features are not fully

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01396.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The α subunit of guanine nucleotide‐binding protein Go(Goα), which was initially isolated from bovine brain, interacts with muscarinic cholinergic receptors and regulates neuronal calcium channels. Goα is known to be localized in neural tissues, some endocrine cells, and neuroendocrine tumors. We have immunohistochemically investigated the expression of Goα in 4 cases of Merkel cell carcinoma using the method of microwave treatment. In all cases of Merkel cell carcinoma, Goα was consistently detected on the plasma membrane and cytoplasm of the tumor cells. Nerve fibers in the skin were also positive for Goα, but other epidermal or dermal components such as keratinocytes, melanocytes, fibroblasts, or lymphoid cells were negative. Tumor cells of squamous cell carcinoma, cutaneous lymphoma, sweat gland carcinoma, and malignant melanoma were negative for Go4aL. The present study indicates that Goα may be a useful immunohistochemical marker of Merkel cell c

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01397.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Androgen receptor (AR) expression was examined in normal skin and in 52 cases of various skin appendage tumors using a monoclonal antibody (F39.4.1) raised against the N‐terminal domain of human AR. Microwave oven heating in citrate buffer solution followed by immunostaining with the labeled streptavidin biotin (LSAB) method was applied to formalin‐fixed paraffin‐embedded sections. Immunoreactive AR was restricted to the nuclei. In normal skin, AR was consistently localized in seboblasts and in some differentiated sebocytes, and variable expression was seen in luminal epithelial cells of eccrine and apocrine glands in the secretory portion. Hair follicles and epidermis showed no reactivity. In sweat gland tumors, AR was identified locally in inner layer cells of the tubuloglandular component of ten of thirteen chondroid syringomas but the remaining tumors were nonreactive. In sebaceous gland tumors, benign tumors with mature sebaceous elements (sebaceous nevi and sebaceous adenomas) showed AR expression, but the sebaceous epitheliomas and sebaceous carcinomas lost their expression. No AR expression was observed in hair follicle tumors, except in AR‐positive mature sebaceous glands incorporated into the cyst wall of steatoc

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01398.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Histologic studies have become increasingly important in recognizing morphologic differences in photoaged versus intrinsically aged skin. Earlier histologic studies have attempted to evaluate these changes by examining anatomical sites which are not comparable, such as face and buttocks. As part of a multicenter study, we have quantitatively examined a panel of 16 histologic features in baseline facial skin biopsies from 158 women with moderate to severe photodamage. When compared to the postauricular area (photo protected), biopsies of the crow's feet area (photo exposed) had a twofold increase in melanocytes and a statistically significant increase in melanocytic atypia (p<.0001) and epidermal melanin (p<.0001). Other epidermal changes included reduced epidermal thickness (p<.01), more compact stratum corneum (p<.0001) and increased granular layer thickness (p<.0001) in the crow's feet skin. There was increased solar elastosis (p<.0001), dermal elastic tissue (p<.0001), melanophages (p<.0001), perivascular inflammation (p<.05) and perifollicular fibrosis (p<.01) but no change in the number of mast cells or dermal mucin in the photo exposed skin. Our data document quantitative differences in photoaged versus intrinsically aged facial skin and provides the groundwork for future studies to evaluate the efficacy of new treatments for photoaged skin.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01399.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Facial inflammatory dermatoses can be difficult to diagnose in their early stages; skin biopsy may help but often shows indeterminate features. The aims of our study were to identify patients presenting with a facial dermatosis requiring skin biopsy, in whom the initial histological features were classified as “non‐specific”, to determine the eventual diagnostic outcome, and to attempt to identify subtle early histological features. We located 79 such cases with indeterminate histology from 45,000 dermatopathology reports 1972‐1987. The original biopsies were assessed and scored using a check‐list of 20 features and the patients' case‐records up to 1992 were examined. Follow‐up information was available in 64 cases and an eventual clinical diagnosis was made in 54 (84%) of these; the commonest diagnoses were rosacea (26%), chronic discoid lupus erythematosus (24%) and seborrheic or unclassified dermatitis (17%). Histological differences were seen in the original biopsies of these three diagnostic categories, although none was predictive. We conclude that clinical follow‐up leads to the diagnosis of most facial rashes initially showing “nonsp

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1995.tb01400.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY