摘要:

Any pathogenetic mechanism proposed for erythema multiforme (EM) must account for the prominent mononuclear cell infiltrate in the skin lesions. The purpose of this study was to characterize immunopathologically, with monoclonal antibodies to human leukocyte antigens, the inflammatory cells in early target lesions of recurrent herpes‐associated EM. Cryostat sections of snap‐frozen skin biopsies were studied by the avidin‐biotin immunoperoxidase technique with use of the following monoclonal antibodies: anti‐HLA‐DR, anti‐Leu M5, anti‐Leu 4+5b, anti‐Leu 3a+3b, anti‐Leu 2a, anti‐Leu 14, and anti‐Leu 6. The dermal mononuclear inflammatory infiltrate in the EM biopsies consisted of monocyte‐macrophages and T‐lymphocytes, with both helper and suppressor T cells present. Both the dermal inflammatory infiltrate and the overlying keratinocytes were strongly HLA‐DR positive. No definite alteration of Langerhans cell number or distribution was noted. These findings are consistent with the characteristics seen in cell‐mediated immune reactions in the skin and point to this as a likely immune mechanis

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00497.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The relationship between the disorder known in Japanese and Chinese literature as Kimura's disease and that known in Western literature as angiolymphoid hyperplasia with eosinophilia (ALHE) has been the subject of debate. Many reports have used the terms synonymously. We have reviewed the histological and clinical features of 4 cases, all occurring in Caucasians, 2 of which are typical of Kimura's disease and 2 of ALHE. Analysis of the cases indicates that the histological features of the 2 disorders are sufficiently different to warrant their recognition as 2 distinct entities. The histological and clinical features of Kimura's disease are most consistent with an allergic or autoimmune process in which blood vessels, lymphocytes and eosinophils participate. Those of ALHE suggest a primary, probably neoplastic disorder of vascular endothelium with a variable and secondary inflammatory response. Although there is some clinical overlap between patients with the 2 disorders, the histological features are distinctive, and the 2 terms should not be used synonymously.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00498.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

We report three new cases of neutrophilic eccrine hidradenitis associated with induction chemotherapy which resolves two major points. First, because two of our patients had testicular carcinoma; this firmly establishes that NEH is not exclusively seen in patients with acute myelogenous leukemia or Hodgkin's lymphoma as previously reported. Second, because two of our patients did not receive cytarabine which has previously been suspected of being the causative agent, it is apparent that this disorder may be produced by more than one chemotherapeutic agent or combination of agents. The histologic features with a discussion of the spectrum of changes which may be seen are presented.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00499.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

We have examined 61 histopathologic specimens from 54 patients with cutaneous leukocytoclastic vasculitis (LV). They were examined without knowledge of clinical disease for the following characteristics: vessel wall inflammation, erythrocyte extravasation, depth of infiltrate, leukocytoclasis, fibrinoid necrosis, epidermal necrosis and fibrin thrombi. Independent of the histopathological study, the following clinical observations were recorded: type of lesion, severity and the presence and type of systemic: disease. The severity score was based on the extent of cutaneous disease, the presence of systemic disease and the difficulty of disease control. The clinical and histological features were then compared using an analysis of variance. Statistically significant results included (1) the relation of clinical severity to vessel wall inflammation, leukocytoclasis, overall histologic severity; and (2) the mildness of histological changes in patients with urticarial lesions. Thus, we have demonstrated that the severity of histopathologic changes seen on skin biopsy is (1) predictive of clinical severity, (2) may correlate with the type of skin lesion observed, but (3) does not correlate with the presence or absence of internal vasculitic involvement.

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00500.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Immunohistochemical distribution of carbonic anhydrase II (CA) in mixed tumours and adenomas of sweat gland origin and in sebaceous adenomas was demonstrated by the PAP method. Normal sweat glands, both eccrine and apocrine, clear cells of the secretory coils, and ductal epithelial cells all showed conspicuous staining for CA, and sebaceous glands were also positive. Mixed tumours of the skin indicated strongly positive staining for CA in the luminal cells of tubular and duct‐like or cystic structures, while most of the other tumour cells were negative. In solid or massive foci, CA positive cells were found scattered among the cellular mass. Sebaceous adenomas were usually moderately positive for CA throughout the tumou

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00501.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Cowden's disease is characterized by multiple hamartomas of the skin, breast, thyroid, and gastrointestinal tract. In the past, a viral hypothesis for the keratotic lesions of the skin has led to much controversy. The present study describes the results of a detailed fine structural analysis of 10 hyperkeratotic extremity lesions and 2 keratotic lesions from the face of a patient with Cowden's disease. Increases in the keratinocyte population were primarily confined to the basal and suprabasal regions. Differentiation products characteristic of keratinization were normal in both quantity and appearance. Nuclear remnants and number‐ous lipid droplets, markers of abnormal keratinization, were noted within horny cells. However, viral particles and/or virus‐like particles were not observed in keratinocytes. Melanocytes and Langerhans cells were numerous. The latter contained membrane‐bound pigment vacuoles in addition to the characteristic Birbeck granules. These unusual Langerhans cells were observed in the dermis as well as the epidermis. A large number of fully granulated ‘resting” mast cells was uniformly distributed throughout the dermis, associated with a prominent cellular infiltrate. Our observations do not support the concept of a viral etiology for the

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00502.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Papillomavirus infects human epidermal cells and causes verruca vulgaris, which is characterized by altered epidermal growth rates and differentiation patterns. There is also a prominent induced dermal proliferative response. Ten formalin‐fixed skin specimens from 10 patients, each with histologically characteristic verruca vulgaris, were excised from paraffin blocks, deparaffinized in xylene, and rehydrated in graded ethanol solutions. Hydrated specimens were incubated in 90% formic acid at 45°C for 18 h. Using a dissecting microscope the epidermis was gently separated from the dermis, which then was fixed in 3% glutaraldehyde and processed for scanning electron microscopy. Scanning electron microscopy showed a well‐preserved epidermal‐dermal junction (EDJ) covered by basal lamina. In comparison with normal EDJ, the EDJ of verruca vulgaris showed markedly elongated dermal papillae. Some papillae were broad and thin, and others were broad and thick. Irregularly shaped and branching secondary papillae were common. Also, there were changes in the basal lamina: the normal corrugation pattern was replaced by relatively smooth undulations or coarse, vertically oriented ridges on papillae. Papillary tips were often smooth. This study shows that there were prominent alterations in the topography of the EDJ and basal lamina in verruca vulgaris. This technique should be useful in evaluating epithelial‐connective tissue morphologic interactions in formalin fixed archival tissue in other diseases characterized by alterations in epidermal growth rates and differentiation

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00503.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A case of annular elastolytic giant cell granuloma was studied with the electron microscope using acid orcein stain. Elastic fibers were digested by multinucleate giant cells (MGCs) in 2 steps: the first step was the extracellular digestion and the second was the intracellular digestion. In the first step, the orcein‐reactive amorphous materials of the elastic fibers surrounded by MGCs at first disappeared, and then the fine filaments appeared in elastic fibers. This process advanced in extracellular spaces of MGCs. In the next step, the fine filaments were phagocytosed by MGCs and were digested in phagocytic vacuole

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00504.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The skin and cultured fibroblasts from a patient with I‐cell disease were examined by electron microscopy. Multiple vacuolations were seen in fibroblast and/or histiocyte‐like cells, secretory cells of eccrine glands, and Schwann cells of the skin. Vacuolar inclusions were single membrane‐limited, and contained a few reticulo‐floccular and vesicular materials, endothelial cells of the dermal capillaries contained other types of inclusions, which were also membrane‐limited, more electron dense, and multivesicular. The epidermis and pilosebaceous appendages seemed to be normal. Cultured skin fibroblasts contained prominent inclusions which varied in size and morphology. Acid phosphatase activity was seen in some of those inclusions, indicating their derivation from lysosomes. These findings suggest that the I‐cell disease is a type of lysosomal storage disease and that electron microscopic examination of normal‐appearing skin in this disease may contribute to

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00505.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0303-6987    

DOI:10.1111/j.1600-0560.1987.tb00506.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY